Protective effect of Omega-3 fatty acids on blood lipid profile of cigarette smoke exposed rats


Affiliations

  • Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B.R.AmbedkarUniversity, Agra, U.P, 282002, India

Abstract

The present study is undertaken to investigate the role of Omega-3 fatty acids on blood lipid profile viz serum cholesterol, serum triglyceride, high density lipoprotein (HDL), low density lipoprotein (LDL), and very low density lipoprotein (VLDL) in cigarette smoke exposed albino rats. Experimental rats (100-158g) were kept in standard laboratory conditions and grouped into three sets - control set A exposed to ambient air, experimental set (B) was exposed to cigarette smoke for one hour per day for 28 days and experimental set (C) was exposed to cigarette smoke with pre-exposure supplementation of Omega-3 fatty acids. A significant decrease in serum cholesterol (p<0.05), serum triglyceride (p<0.05), LDL (p<0.05) and VLDL (p<0.05) with a significant increase in HDL level (P<0.05) after supplementation of Omega-3 fatty acids in comparison to cigarette smoke exposed albino rats.

Keywords

Cigarette smoke, Albino rat. Blood lipid profile, Omega-3 fatty acids

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References

Ambrose, J.A, and Barua, R.S. (2004) The pathophysiology of cigarette smoking and cardiovascular disease. J. Am.Coll. Cardiol., 43, 1731-1737.

Anbarasi, K., Sabitha, K.E. and Shyamala Devi, C.S. (2005) Lactate dehydrogenase isoenzyme patterns upon chronic exposure to cigarette smoke: Protective effect of bacoside A. Environment Toxicol. Pharmacol. 20, 345-350.

Friedwald, W.T., Levy, R.I. and Fredrickson, D,S. (1972) Estimation of the concentration of lov; density lipoprotein cholesterol in plasma without use of the preparative ultracentrifuge. Clin. Chem., 18, 499,

Garrison, R.J., Kannel, W.B., Feinleib, M., Castelll, W.P, McNamara, P.M. and Padget, S.J, (1978) Cigarette smoking and HDL-cholestrol. The Framingham offspring study. Atherosclerosis, 30, 17-25.

Halliwell, B. and Gutteridge, J.M.C. (1989) Free radicals in biology and medicine (2"° ed.), Oxford, U.K., Clarendon.

Harman, D. (1992) Role of free radicals in ageing and disease, Ann. N.Y. Acad. Sci.. 673, 126-141.

Heitzer, T. and Munzel, H. (1996) Antioxidant vitamin C-improves endothelial dysfunction in chronic smokers. Circulation, 94, 6-9

Kamisaki, Y, Wada, K., Nakamoto. Y., Ashida, K. and Itoh, T (1997) Substances in the aqueous fraction of cigarette smoke inhibit lipid peroxidation in synaptosomes of rat cerebral cortex. Biochem. Mol. Biol. Int., 42, 1-10.

Kharb, S. and Singh, G.P (2000) Effect of smoking on lipid profile, lipid peroxidation and antioxidant status in normal subjects and in patients during and after acute myocardial infarction. Clin. Chem. Acta. 302, 213-219,

Lakier, J.B. (1992) Smoking and cardiovascular disease. Am.J. Med., 93, 8-12.

Nishio, F. and Watanabe, Y (1997) Cigarette smoke extrac. inhibits plasma paraxonase activity by modification of enzymes free thiols Biochem. Biophys. Res. Commun., 236, 289-293.

Orhan, H., Evelo, C.T.A. and Sahin, G. (2005) Erythrocyte antioxidant defense response against cigarette smoking in humans - the glutathione S-transferase vulnerability J.Biochem. Mol. Toxicol., 19, 226-233.

Pant, Wl.C. (2004) Essentials of Biochemistry (9"" ed.). Kedarnath Ramnath and Co. Meerut, pp 670.

Roeschlau, P Bernt, E. and Gruber, W.A. (1974) Clin. Chem. Clin. Biochem., 12, 226-220.

Schettler, G.and Nussel, E. (1975) Determination of triglycerides ARB. Med. SO^ Med. Prav. Med., 10, 25-28.

Stubbe, I., Eskilsson, J. and Nilsson-Ehle, R (1982) High density lipoprotein concentrations increase after stopping smoking.Br Med. J., 284, 1511-1153.

Uauy R. and Valenzuela, A. (2000) Marine oils: The health benefits of Omega-3 fatty acids. Nutrition, 16, 680-684.

Wybenga, DR. and Pileggi (1970) In vitro determination of cholesterol and HDL cholesterol in serum/plasma, Clin. Chem., 16, 980-984.


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