Biodecolorization of Azo Dye Acid Blue 113 by Soil Bacterium Klebsiella variicola RMLP1


  • Dr. Rammanohar Lohia Avadh University, Department of Biochemistry, Ayodhya, Uttar Pradesh, 224001, India
  • Dr. Rammanohar Lohia Avadh University, Department of Biotechnology, Ayodhya, Uttar Pradesh, 224001, India
  • Guru Ghasidas University, Department of Biotechnology, Bilaspur, Chhattisgarh, 495009, India
  • Nilamber-Pitamber University, Palamu, Jharkhand, 822101, India


The present study was aimed to isolate a new bacterial strain for the degradation/decolorization of azo dye Acid Blue 113 (AB 113). The physico-chemical method is inadequate for degradation of azo dyes; therefore, an environmental friendly and competent method such as use of the biological organism was studied for decolorization of AB 113. Bushnell and Hass (BHM) medium containing AB 113 dye were used to perform the decolorization study. 16S rRNA gene sequencing approach was used for identification of bacterial isolate as a Klebsiella variicola. The optimum process parameters for the decolorization of AB 113 were found at pH 8, 35°C temperature and 100 mg/L dye concentration during 72 h incubation. Glucose and ammonium sulphate was the carbon and nitrogen source suited well for the decolorization of dye. The results proved that the Klebsiella variicola, offer huge ability in treating textile wastewater containing the color AB 113.


Acid Blue 113, Azo Dye, Decolorization, Klebsiella Variicola, 16S rDNA

Subject Discipline

Microbiology; Bioremediation

Full Text:


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