Biodecolorization of Azo Dye Acid Blue 113 by Soil Bacterium Klebsiella variicola RMLP1


Affiliations

  • Dr. Rammanohar Lohia Avadh University, Department of Biochemistry, Ayodhya, Uttar Pradesh, 224001, India
  • Dr. Rammanohar Lohia Avadh University, Department of Biotechnology, Ayodhya, Uttar Pradesh, 224001, India
  • Guru Ghasidas University, Department of Biotechnology, Bilaspur, Chhattisgarh, 495009, India
  • Nilamber-Pitamber University, Palamu, Jharkhand, 822101, India

Abstract

The present study was aimed to isolate a new bacterial strain for the degradation/decolorization of azo dye Acid Blue 113 (AB 113). The physico-chemical method is inadequate for degradation of azo dyes; therefore, an environmental friendly and competent method such as use of the biological organism was studied for decolorization of AB 113. Bushnell and Hass (BHM) medium containing AB 113 dye were used to perform the decolorization study. 16S rRNA gene sequencing approach was used for identification of bacterial isolate as a Klebsiella variicola. The optimum process parameters for the decolorization of AB 113 were found at pH 8, 35°C temperature and 100 mg/L dye concentration during 72 h incubation. Glucose and ammonium sulphate was the carbon and nitrogen source suited well for the decolorization of dye. The results proved that the Klebsiella variicola, offer huge ability in treating textile wastewater containing the color AB 113.

Keywords

Acid Blue 113, Azo Dye, Decolorization, Klebsiella Variicola, 16S rDNA

Subject Discipline

Microbiology; Bioremediation

Full Text:

References

Singh PK, Singh RL. Bio-removal of Azo Dyes: A Review. Int J Appl Sci Biotechnol. 2017; 5(2):108–126. https://doi.org/10.3126/ ijasbt.v5i2.16881

Benkhaya S, Mrabet S, El Harfi A. Classifications, properties, recent synthesis and applications of azo dyes. Heliyon. 2020; 6(1): e03271. doi.org/10.1016/j.heliyon.2020.e03271.

Cui D, Cui M, Liang B, Liu W, Tang Z, Wang A. Mutual effect between electrochemically active bacteria (EAB) and azo dye in bio-electrochemical system (BES). Chemosphere. 2020; 239: 124787. doi: 10.1016/j.chemospere.2019.124787.

Sarvajith, Reddy GKK, Nancharaiah YV. Textile dye biodecolorization and ammonium removal over nitrite in aerobic granular sludge sequencing batch reactors. J Hazard Mater. 2017; 342: 536–543. https://doi.org/10.1016/j.jhazmat.2017.08.064

Asad S, Amoozegar MA, Pourbabaee AA, Sarbolouki MN, Dastgheib SMM. Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresour Technol. 2007; 98: 2082–2088. https://doi.org/10.1016/j.biortech.2006.08.020

Singh RL, Singh PK, Singh RP. Recent advances in decolorization and degradation of dyes in textile effluent by biological approaches. Baca Raton New York: CRC Press; 2020. https://doi.org/10.1201/9780429244322

Chung KT, Cerniglia CE. Mutagenicity of azo dyes: structure activity relationships. Mutat. Res. 1992; 277: 201–220. https:// doi.org/10.1016/0165-1110(92)90044-A

Acuner E, Dilek FB. Treatment of Tectilon Yellow 2G by Chlorella vulgaris. Process Biochem. 2004; 39: 623–631. https://doi.org/10.1016/S0032-9592(03)00138-9

Ayed L, Chaieb K, Cheref A, Bakhrouf A. Biodegradation and decolorization of Triphenylmethane dyes by Staphylococcus epidermidis. Desalin. 2010; 260: 137–146. https://doi.org/10.1016/j.desal.2010.04.052

Tochhawng L, Mishra VK, Passari AK, Singh BP. Endophytic Fungi: role in dye decolorization. In: Advances in endophytic fungal research, Singh B. (ed), Fungal biology. Springer, Cham.

; pp1–15. https://doi.org/10.1007/978-3-030-03589-1_1

Singh RL, Singh PK, Singh RP. Enzymatic decolorization and degradation of azo dyes-a review. Int Biodeter Biodegr. 2015; 104: 21–31. https://doi.org/10.1016/j.ibiod.2015.04.027

Guo G, Li X, Tian F, Liu T, Yang F, Ding K, Liu C, Chen J, Wang C. Azo dye decolorization by a halotolerant consortium under microaerophilic conditions. Chemosphere. 2020; 244: 125510. doi:10.1016/j.chemosphere.2019.125510.

Carvalho MC, Pereira C, Gonçalves IC, Pinheiro HM, Santos AR, Lopes A, Ferra MI. Assessment of the biodegradability of a mono-sulfonated azo dye and aromatic amines. Int Biodeter Biodgr. 2008; 64: 676–681.

Barragan BE, Costa C, Marquez MC. Biodegradation of azo dyes by bacteria inoculated on solid media. Dyes Pigments. 2007; 75: 73–81. https://doi.org/10.1016/j.dyepig.2006.05.014

Singh RN, Kaushik R, Arora DK, Saxena AK. Prevalence of opportunist pathogens in thermal springs of devotion. J Appl Sci Environ Sanit. 2013; 8: 195–203.

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics analysis. Version 6.0. Mol Biol Evol. 2013; 30: 2725–2729. https://doi.org/10.1093/molbev/mst197

Zimmermann T, Kulla HG, Leisinger T. Properties of purified Orange II azoreductase, the enzyme initiating azo dye degradation by Pseudomonas KF46. Eur J Biochem. 1982; 129: 197–203.

https://doi.org/10.1111/j.1432-1033.1982.tb07040.x

Kalyani DC, Telke AA, Dhanve RS, Jadhav JP. Eco-friendly Biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1. J Hazard Mater. 2008; 163: 735–742. https://doi.org/10.1016/j.jhazmat.2008.07.020

Aravindhan R, Rao JR, Nair BU. Removal of basic yellow dye from aqueous solution by sorption on green alga Caulerpa scalpelliformis. J Hazard Mater. 2007; 142: 68–76. https://doi.org/10.1016/j.jhazmat.2006.07.058

Kumar K, Devi SS, Krishnamurthi K, Dutta D, Chakrabarti T. Decolorization and detoxification of Direct Blue-15 by a bacterial consortium. Bioresour Technol. 2007; 98: 3168–3171. https://doi.org/10.1016/j.biortech.2006.10.037

Yu ZS, Wen XH. 2005. Screening and identification of yeasts for decolorizing synthetic dyes in industrial wastewater. Int Biodeter Biodegr. 2005; 56: 109–114. https://doi.org/10.1016/j.ibiod.2005.05.006

Asad S, Amoozegar MA, Pourbabaee AA, Sarbolouki MN, Dastgheib SMM. Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresour Technol. 2007; 98: 2082–2088. https://doi.org/10.1016/j.biortech.2006.08.020

Fan L, Zhu SN, Liu DQ, Ni JR. Decolorization of 1-amino-4-bro-moanthraquinone-2-sulfonic acid by a newly isolated strain of Sphingomonas herbicidovorans. Int Biodeter Biodegr. 2009; 63: 88–92. https://doi.org/10.1016/j.ibiod.2008.07.004

Ghodake G, Jadhav U, Tamboli D, Kagalkar A, Govindwar S. Decolorization of textile dyes and degradation of mono azo dye Amaranth by Acinetobacter calcoaceticus NCIM 2890. Indian J Microbiol. 2011; 51: 501–508. https://doi.org/10.1007/s12088011-0131-4

Isik M, Sponza DT. Effect of oxygen on decolorization of azo dyes by Escherichia coli and Pseudomonas sp. and fate of aromatic amines. Process Biochem. 2003; 38: 1183–1192. https://doi.org/10.1016/S0032-9592(02)00282-0

Wang H, Su JQ, Zheng XW, Tian Y, Xiong XJ, Zheng TL. Bacterial decolorization and degradation of the reactive dye Reactive Red 180 by Citrobacter sp. CK3. Int Biodeter Biodegr. 2009; 63: 395– 399. https://doi.org/10.1016/j.ibiod.2008.11.006

Singh RP, Singh PK, Singh RL. Bacterial decolorization of textile azo dye Acid Orange by Staphylococcus hominis RMLRT03. Toxicol. Int. 2014; 21:160–166. https://doi.org/10.4103/09716580.139797

Stolz A. Basic and applied aspects in the microbial degradation of azo dyes. Appl Microbiol Biotechnol. 2001; 56:69–80. https://doi.org/10.1007/s002530100686

Ponraj M, Gokila K, Zambare V. Bacterial decolorization of textile dye Orange 3R. Int J Adv Biotechnol Res. 2011; 2:168–177.

Das A, Mishra S, Verma VK. Enhanced biodecolorization of textile dye Remazol Navy Blue using an isolated bacterial strain Bacillus pumilus HKG212 under improved culture conditions. J Biochem Technol. 2015; 6:962-969.

Singh S, Chatterji S, Nandini PT, Prasad ASA, Rao KVB. Biodegradation of azo dye Direct Orange 16 by Micrococcus luteus strain SSN2. Int J Environ Sci Tech. 2015; 12: 2161–2168.

https://doi.org/10.1007/s13762-014-0588-x

Arulazhagan P. A study on microbial decolorization of Reactive Red M8B by Bacillus subtilis isolated from dye contaminated soil samples. Int J Curr Res Biol Med. 2016; 1:1–13.

Prasad ASA, Rao KVB. Aerobic biodegradation of azo dye Acid Black 24 by Bacillus halodurans. J Environ Biol. 2014; 35: 549– 554.

Tripathi A, Srivastava SK. Eco-friendly treatment of azo dyes: Biodecolorization using bacterial strains. Int J Biosci Biochem Bioinform. 2011; 1:37–40. https://doi.org/10.7763/IJBBB.2011.V1.7

Carolin CF, Kumar PS, Joshiba GJ. Sustainable approach to decolorize Methyl Orange dye from aqueous solution using novel bacterial strain and its metabolites characterization. Clean Technol Envir. 2020; doi.org/10.1007/s10098-020-01934-8.

Banat IM, Nigam P, Singh D, Marchant R. Microbial decolorization of textile dye containing effluents: A review. Bioresour Technol. 1996; 58(3): 217–227. https://doi.org/10.1016/S09608524(96)00113-7

Garg SK, Tripathi M, Singh, SK, Tewari JK. Biodecolorization of textile dye effluent by Pseudomonas putida SKG-1 (MTCC 10510) under the conditions optimized for monoazo dye Orange II color removal in simulated minimal salt medium. Int Biodeter Biodegr. 2012; 74:24–35. https://doi.org/10.1016/j.ibiod.2012.07.007

Saratale RG, Saratale GD, Kalayani DC, Chang JS, Govindwar SP. Enhanced decolorization and biodegradation of textile azo dye Scarlet R by using developed microbial consortiumGR. Bioresour Technol. 2009; 100: 2493–2500. https://doi.org/10.1016/j.biortech.2008.12.013


Refbacks

  • There are currently no refbacks.