Triacontanol Improves Production of Anticancer Alkaloids in Catharanthus roseus L.


Affiliations

  • Aligarh Muslim University, Department of Botany, Plant Physiology Section, Aligarh, Uttar Pradesh, India
  • University of Tabuk, Faculty of Science, Department of Biology, Tabuk, 71491, Saudi Arabia
  • Malda College, Department of Botany, Malda, West Bengal, India
  • Aligarh Muslim University, Botany Section, Women's College, Aligarh, Uttar Pradesh, India

Abstract

Catharanthus roseus (L.) G. Don is a medicinal plant that bears indole alkaloids used in cancer chemotherapy. The anticancer alkaloids, viz. vinblastine and vincristine, are mainly present in leaves of C. roseus. As there is high demand and low yield of these alkaloids in C. roseus, alternative ways to improve alkaloid production are needed. Hence, it was hypothesized that Triacontanol (TRIA), a potent plant growth promoting substance for various medicinal and agricultural crops, would improve alkaloid production in C. roseus. A pot culture experiment was carried out and the effects of TRIA on production of total alkaloids including anticancer alkaloids were evaluated at 120 and 150 days after planting. Four concentrations of TRIA [10-0 (Control) 10-7, 10-6 and 10-5 M] were tested through leaf spraying. TRIA at 10-6 M significantly increased yield attributes. As compared to the control (10-0 M), leaf-applied TRIA at 10-6 M improved the production (yield) of anticancerous alkaloids vinblastine (+71.6%) and vincristine (+73.1%) and caused the highest content and yield of vindoline.


Keywords

Catharanthus roseus, Triacontanol (TRIA), Vinblastine, Vincristine, Vindoline Alkaloids

Subject Discipline

Botany

Full Text:

References

Gueritte F, Fahy J. The vinca alkaloids. In: G.M., Cragg, D.G.I., Kingston, D.J., Newman, (Eds.), Anticancer Agents from Natural Products. Runner-Routledge Psychology Press, Taylor and Francis Group, Boca Raton, FL; 2005. 123−36. https://doi.org/10.1201/9781420039658.ch7.

Oksman-Caldentey KM, Inze D. Plant cell factories in the post-genomic era: New ways to produce designer secondary metabolites, Trends in Plant Science. 2004; 9:433−440.

https://doi.org/10.1016/j.tplants.2004.07.006. PMid: 15337493.

Zhao J, Verpoorte R. Manipulating indole alkaloid production by Catharanthus roseus cell cultures in bioreactors: From biochemical processing to metabolic engineering, Phytochemistry Review. 2007; 6:435−57. https://doi.org/10.1007/s11101-006-9050-0.

Facchini PJ, Huber-Allanach KL, Tari LW. Plant aromatic L-amino acid decarboxylases: Evolution, biochemistry, regulation, and metabolic engineering applications, Phytochemistry. 2000; 54:121−38. https://doi.org/10.1016/ S0031-9422(00)00050-9.

Ries SK, Houtz RL. Triacontanol levels in ascending sugar maple sap, Hort. Science. 1983; 18:101−02.

Ries S. Triacontanol and its second messenger 9-β-L(+)adenosine as plant growth substances, Plant Physiology. 1991; 95:986−89. https://doi.org/10.1104/pp.95.4.986. PMid: 16668132.

Naeem M, Khan MMA, Moinuddin. Triacontanol: A potent plant growth regulator in agriculture, Journal of Plant Interactions. 2012; 7:129−42. https://doi.org/10.1080 /17429145.2011.619281.

Khan R, Khan MMA, Singh M, Nasir S, Naeem M, Siddiqui MH, Mohammad F. Gibberellic acid and triacontanol can ameliorate the optimum yield and morphine production in opium poppy (Papaver somniferum L.), Acta Agriculturae Scandinavica, Section B-Soil and Plant Science. 2007; 57:307−12. https://doi.org/10.1080/09064710600982811.

Aftab T, Khan MMA, Idrees M, Naeem M, Singh M, Ram M. Stimulation of crop productivity, photosynthesis and artemisinin production in Artemisia annua L. by triacontanol and gibberellic acid application, Journal of Plant Interactions. 2010; 5:273−81. https://doi.org/10.1080/17429141003647137.

Misra A, Srivastava NK. Effects of the triacontanol formulations “Miraculan” on photosynthesis, growth, nutrient uptake, and essential oil yield of lemongrass (Cymbopogon flexuosus) Steud, Watts, Plant Growth Regulation. 1991; 10:57−63. https://doi.org/10.1007/BF00035131.

Srivastava NK, Sharma S. Effect of triacontanol on photosynthetic characters and essential oil accumulation in Japanese mint (Mentha arvensis L.), Photosynthetica. 1991; 25:55−60.

Naeem M, Aftab T, Ansari AA, Idrees M, Ali A, Khan MMA, Uddin M, Varshney L. Radiolytically degraded sodium alginate enhances plant growth, physiological activities and alkaloids production in Catharanthus roseus L, Journal of Radiation Research and Applied Sciences. 2015a; 8:606−16.

https://doi.org/10.1016/j.jrras.2015.07.005.

Naeem M, Idrees M, Aftab T, M. Masidur Alam MM, Khan MMA, Uddin M, Varshney L. Radiation processed carrageenan improves plant growth, physiological activities and alkaloids production in Catharanthus roseus L, Advances in Botany Article ID 150474. 2015; 2015b:1−11. https://doi.org/10.1155/2015/150474.

Uniyal GC, Bala S, Mathur AK, Kulkarni RN. Symmetry C18 column: a better choice for the analysis of indole alkaloids of Catharanthus roseus, Phytochemical Analysis. 2001; 12:206−10. https://doi.org/10.1002/pca.575. PMid: 11705028.

Idrees M, Khan MMA, Aftab T, Naeem M. Synergistic effects of gibberellic acid and triacontanol on growth, physiology, enzyme activities and essential oil content of Coriandrum sativum L, The Asian and Australasian Journal of Plant Science and Biotechnology. 2010; 4:24−29.

Karam EA, Keramat B, Asrar Z, Mozafari H. Triacontanolinduced changes in growth, oxidative defense system in coriander (Coriandrum sativum) under arsenic toxicity, Physiol. Mol. Biol. Plants. 2016; 22:341−49. https://doi.org/10.1007/s40502-016-0213-8.

Naeem M, Khan MMA, Moinuddin, Idrees M, Aftab T. Triacontanol-mediated regulation of growth and other physiological attributes, active constituents and yield of Mentha arvensis L, Plant Growth Regulation. 2011; 65:195−206. https://doi.org/10.1007/s10725-011-9588-8.

Srivastava NK, Sharma S. Effect of triacontanol on photosynthesis, alkaloid content and growth in opium poppy (Papaver somniferum L), Plant Growth Regulation. 1990; 9:65−71. https://doi.org/10.1007/BF00025280.

Nasir S. Influence of Triacontanol and Macronutrient Elements on the Growth, Yield and Alkaloid Content of Withania Somnifera Dunal. L. and Datura Innoxia Mill. PhD Thesis, AMU, Aligarh, India; 2009.

Singh P, Srivastava NK, Mishra A, Sharma S. Influence of ethrel and gibberellic acid on carbon metabolism, growth, essential oil accumulation in spearmint (Mentha spicata), Phtosynthetica. 1999; 36:509−17. https://doi.org/10.1023/A:1007083802765.

Naeem M, Khan MMA, Moinuddin, Siddiqui MH. Triacontanol stimulates nitrogen-fixation, enzyme activities, photosynthesis, crop productivity and quality of hyacinth bean (Lablab purpureus L.), Scientia Horticulturae. 2009; 121:389−96. https://doi.org/10.1016/j.scienta.2009.02.030.

Naeem M, Idrees M, Aftab T, Khan MMA, Moinuddin. Changes in photosynthesis, enzyme activities and production of anthraquinone and sennoside content of coffee senna (Senna occidentalis L.) by triacontanol, International Journal of Plant Developmental Biology. 2010; 4:53−59.

Moreno PRH, Van der Heijden R, Veerporte R. Cell and tissue cultures of Catharanthus roseus, Plant Cell and Tissue Organ Culture. 1995; 42:1−25. https://doi.org/10.1007/ BF00037677.

Idrees M, Naeem M, Aftab T, Khan MMA, Moinuddin. Salicylic acid mitigates salinity stress by improving antioxidant defence system and enhances vincristine and vinblastine alkaloids production in periwinkle [Catharanthus roseus (L.) G. Don], Acta Physiologiae Plantarum. 2011; 33:987−99.

https://doi.org/10.1007/s11738-010-0631-6.

Srivastava NK, Srivastava AK. Influence of gibberellic acid on 14CO2 metabolism, growth, and production of alkaloids in Catharanthus roseus, Photosynthetica. 2007; 45:156−60.

https://doi.org/10.1007/s11099-007-0026-0.

Misra P, Kumar S. Emergence of periwinkle (Catharanthus roseus) as a model system for molecular Biology of alkaloid: Phytochemistry, pharmacology, plant biology and in vivo and in vitro cultivation, J. Med. Arom. Plant Sci. 2000; 22:306−37.

El-Sayed M, Verpoorte R. Growth, metabolic profiling and enzymes activities of Catharanthus roseus seedlings treated with plant growth regulators, Plant Growth Regulation.

; 44:53−58. https://doi.org/10.1007/s10725-004-2604-5.

Ataei-Azimi A, Hashemloian BD, Ebrahimzadeh H, Majd A. High in vitro production of ant-canceric indolealkaloids from periwinkle (Catharanthus roseus) tissue culture, African Journal of Biotechnology. 2008; 7:2834−39.

Alam M, Naeem M, Idrees M, Khan MMA, Moinuddin. Augmentation of photosynthesis, crop productivity, enzyme activities and alkaloids production in sadabahar (Catharanthus roseus L.) through application of diverse plant growth regulators, Journal of Crop. Science and Biotechnology. 2012; 15:117−29. https://doi.org/10.1007/s12892-011-0005-7.


Refbacks

  • There are currently no refbacks.