Qualitative Analysis and Anthelmintic Activity of Hydro-Alcoholic Extract of Tabernaemontana Divaricata


Affiliations

  • School of Chemical and Biotechnology, SASTRA University, Department of Pharmaceutical Technology, Thanjavur, 613401, India

Abstract

Tabernaemontana divaricata is a common shrub found in the tropical regions and is often used for medicinal purposes, particularly the flowers of the plant. The present study is conducted to compare and identi fy the phytochemical constituents by Thin Layer Chromatography (TLC) and Qualitative Phytochemical analysis and to determine the anthelmentic activity of fresh and dried flower extract of Tabernaemontana divaricata . The extract is obtained using two differ ent methods like cold maceration and hot solvent extraction by using soxhlet apparatus, first with petroleum ether followed by hydroalcohol as solvents. The preliminary phytochemical analysis of the extract indicated the presence of Alkaloids, Flavanoids, Steroids, Proteins, Carbohydrates and Tannins. The Rf value of TLC is calculated and compared with standard values and analysis proved the presence of the phytochemical constituents. The anthelmentic activity studies are performed using Indian earth worms . For this, the concentrated extract is diluted to various concentrations, and the effect of each solution is studied by measuring the time taken for paralysis and death of the earth worms. It is found to show significant anthelmentic activity at various concentrations compared with that of the standard drug Metronidazole.

Keywords

Thin Layer Chromatography, Eudrilus Eugeniae, Eisenia Foetid.

Subject Discipline

Pharmacy and Pharmacology

Full Text:

References

Soriful Islam M, Most. Mauluda Akhtar, Mostafizur Rahman M, Atikur M, et al, Antitumor and Phytotoxic Activities of Leaf Methanol Extract of Oldenlandia diffusa (Willd.) RoxbGlobal Journal of Pharmacology, 2009;3 (2): 99-106.

Das BK, Das B, Arpita FK, Hannan JMA et al, Phytochemical Screening And Antioxidant Activity Of Leucas Aspera, IJPSR, 2011;2(7):.

Joy PP, Thomas Samuel Mathew J, Baby, et al, Medicinal plants, 1998.

Rahman MD. Ashikur MD. Hasanuzzaman Rahman MD et al, Evauation of anibacterial activity of study of leaves of Tabernaemontana divaricata (L), IRJP, 2011; 2(6): 123-127.

Basavaraj P, Shivakumar B, Shivakumar H. Anxiolytic Activity Of Tabernaemontana divaricata (Linn) R.Br. Flowers extract in mice. International Journal of Pharma and Bio Sciences, 2011, 2 (3).

Orient Longman, Indian Medicinal Plants, a compedium of 500 species, 1996, 5, 232-234.

Masudur Rahman Md, Saiful Islam Md, Sekendar Ali Md, et al, Antidiabetic and Cytotoxic Activities of Methanolic Extract of Tabernaemontana divaricata (L.) Flowers, International Journal of Drug Development & Research, 2011; 3:

Basavaraj P, Shivakumar B, Shivakumar H, Manjunath V J et al, Evaluation Of Anticonvulsant Activity Of Tabernaemontana Divaricata (Linn) R. Br. Flower Extract. International Journal of Pharmacy and Pharmaceutical Sciences, 2011, 3(3).

Abah SE, Egwari LO, Methods of extrraction and Antimicrobial succesptibility testing of plant extracts,African journal of basic and applied sciences,2011;3(5):205-209.

Gupta Shailesh and Kohli Seema, Phytochemical screening of Sarcostigmma acidum W. & Ar., International journal of pharmacy & life sciences, 2011; 4.

Dutta Choudhury M, Katyayani Dutta Choudhury, TLC Profiling of Lasianthus lucidus Blume.(Rubiaceae). Assam University Journal of Science & Technology , 2011;7:114-117.

Sahu Vinod K, Irchhaiya Raghuveer, Shashi Alok, Gurjar Himanshu Phytochemical investigation and chromatographic evaluation of the ethanolic extract of whole plant extract of Dendrophthoe falcata (l.f.), IJPSR (2010);1 (1): 40-45.

Das Talukdar A, Dutta Choudhury M, Chakraborty M, et al, Phytochemical screening and TLC profiling of plant extracts of Cyathea gigantea (Wall. Ex. Hook.) Haltt. and Cyathea brunoniana. Wall. ex. Hook. (Cl.

& Bak.), Assam University Journal of Science & Technology, 2010; 5 (10):70-74.


Refbacks

  • There are currently no refbacks.