Frequency of Microsporidial Infection in Immunocompromised Patients with Staining and Molecular Methods Based on Internal Transcribed Spacer Region Gene in Two Cities of Southwest Iran during 2013-2014


Affiliations

  • Ahvaz Jundishapur University of Medical Sciences, Department of Medical Parasitology and Mycology, Abadan Arvand International Division, Ahwaz, Iran, Islamic Republic of
  • Ahvaz Jundishapur University of Medical Sciences, Health Research Institute, Infectious and Tropical Diseases Research Center, Ahwaz, Iran, Islamic Republic of
  • Ahvaz Jundishapur University of Medical Sciences, Department of Medical Parasitology, Faculty of Medicine, Ahwaz, Iran, Islamic Republic of
  • Ahvaz Jundishapur University of Medical Sciences, Abadan Arvand International Division, Ahwaz, Iran, Islamic Republic of

Abstract

Microsporidiosisis anopportunistic infection frequently diagnosed in immunocompromised patients. Microsporidiosis causes symptomatic or asymptomatic infections immunocompromised/immunosuppressed patients may experience severe and disseminated microsporidiosis. Fecal samples were taken from 186 patients with human immunodeficiency virus (86) and cancer (100). Microsporidia spp. were detected 27.9% (24/86) and 11.6% (10/86) in HIV + patients, 18% (18/100) and 7% (7/100) of cancer patients using polymerase chain reaction (PCR) and trichrome staining respectively. Enterocytozoon bieneusi was detected in 28(66.7%) patients, while Encephalitozoon intestinalis was detected in 10 (23.8%) patients. Encephalitozoon cuniculi was detected in only one patient, while Encephalitozoon hellem was detected in three patients. Microsporidia was significantly higher in patients with diarrhea than those without diarrhea (P = 0.001). CD4 T cells blood count was significantly lower (<200 cells/μl blood) in HIV+ patients with Microsporidia (P = 0.001). Also, there was significant difference between contact with animals with positive results of HIV+ and cancer patients (P< 0.005). This study confirmed that PCR technique was more sensitive than trichrome staining for diagnosis. Moreover, intestinal microsporidiosis caused diarrhea in HIV+ and cancer patients. This influenced treatment. Therefore, early diagnosis of microsporidiosis is effective on treatment strategies.

Keywords

HIV, Immunocompromised Patients, Iran, Microsporidia, PCR

Subject Discipline

Pharmacy and Pharmacology

Full Text:

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