Effects of LED Irradiation on the Proinflammatory Cytokines Production


  • Nambu University, Department of Nursing, Gwangju, Korea, Republic of


Although the anti-inflammatory effects have been reported in clinical fields for specific wavelength irradiation during wound healing, the physiological mechanism has not been clarified yet. The aim of the present study is to investigate the anti-inflammatory effect of Light-Emitting-Diode (LED) irradiation. The present study was designed to determine the effect of LED irradiation on TNF-α, IL-1β and IL-6 Detection and NO production in Lipopolysaccharide (LPS) - stimulated RAW264.7 macrophages. Cell toxicity was determined by MTS assay. The amount of NO was measured using the NO Detection Kit and the ELISA was performed by coating 96-well plates of monoclonal antibody with specificity for TNF-α and IL-6 respectively. 440nm LED irradiation reduced TNF-α and IL-6 Detection and NO production without cytotoxity. Our results suggest that 440nm LED irradiation may have an anti-inflammatory property through suppressing inflammatory mediator productions and appears to be useful as an anti-inflammatory tool.


Inflammation, LED (Light Emitting Diode), Light Therapy, NO

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