Effectiveness of Various Disinfectant Protocols on Orthodontic Pliers – An In-Vitro Study


  • Kerala University of Health Sciences, Department of Orthodontics, Azeezia College of Dental Sciences and Research, Kollam, Kerala, 691537, India
  • Kerala University of Health Sciences, Department of Microbiology, Azeezia Institute of Medical Sciences and Research, Kollam, Kerala, 691537, India


Disinfection of the instruments used in orthodontic clinic is essential in order to prevent the transmission of disease to the practitioner, auxiliary staff and patients. Present study aims to evaluate the effectiveness of 2% Glutaraldehyde solution, quaternary ammonium compound based wipes and foam sprays on the disinfection of orthodontic pliers. Methodology: The efficacy of disinfection methods of orthodontic pliers used in everyday practice by orthodontists was evaluated using 30 sterile pliers. They were contaminated in vitro with bacteria commonly found in the oral cavity, Streptococcus salivarius [ATCC 13419], Staphylococcus aureus [ATCC 25923] and Pseudomonas aeruginosa [ATCC27853]. Following contamination of the pliers, each of ten pliers underwent one of the three disinfectant protocols in a solution of 2% Glutaraldehyde, alcohol-free wipes containing quaternary ammonium compounds and spraying a foam containing quaternary ammonium compound. The plier heads were spread on the surface of sterile blood agar plates after contamination and after disinfection. Results: 2% Glutaraldehyde solution, quaternary ammonium compound based wipes and foam sprays were found to be effective with regards to disinfection of orthodontic pliers. Conclusion: As per the results of our study, we conclude that 2% Glutaraldehyde solution, quaternary ammonium compound based wipes and foam spray were found to be effective with regards to disinfection of orthodontic pliers contaminated with standard strains of Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus salivarius.


Glutaraldehyde, Pseudomonas aeruginosa, Quaternary Ammonium Compounds, Staphylococcus aureus, Sterilization, Streptococcus salivarius

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Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE. Defining the normal bacterial flora of the oral cavity. J. Clin. Microbiol. 2005 Nov; 43(11):5721-32. https://doi.org/10.1128/JCM.43.11.5721-5732.2005.

PMid: 16272510 PMCid: PMC1287824.

Malamud D, Rodriguez-Chavez IR. Saliva as a Diagnostic Fluid. Dent. Clin. North Am. 2011 January; 55(1):15978. https://doi.org/10.1016/j.cden.2010.08.004. PMid: 21094724, PMCid: PMC3011946.

Kirchhoff ST. Sterilization in orthodontics. J. Clin. Orthod. 1987 May; 21(5):326-28.

Kalra S, Tripathi T, Rai P. Infection control in orthodontics. Journal of Orthodontics and Endodontics. 2015; 1(1):1.

Gendron R, Grenier D, Maheu-Robert L. The oral cavity as a reservoir of bacterial pathogens for focal infections. Microbes Infect. 2000 Jul; 2(8):897-906. https://doi.org/10.1016/S1286-4579(00)00391-9.

Almeida, Camilla Machado Feitosa de, Carvalho, Adriana Silva de, Duarte, Danilo Antônio. Evaluation of disinfection methods of orthodontic pliers. Dental Press Journal of Orthodontics. 2012; 17(4):105-09. https://doi.org/10.1590/S2176-94512012000400020.

Starnbach H, Biddle P. A pragmatic approach to asepsis in the orthodontic office. Angle Orthod. 1980 Jan; 50(1):6366.

Bhatnagar S, Bagga DK, Sharma P, Kumar P, Sharma R, Singh V. Infection control strategy in orthodontic office. Eur. J. Gen. Dent. 2013; 2:1-7. https://doi.org/10.4103/22789626.106793.

Wichelhaus A, Bader F, Sander FG, Krieger D, Mertens T. Effective disinfection of orthodontic pliers. J. Orofac. Orthop. 2006 Sep; 67(5):316-36. https://doi.org/10.1007/s00056-006-0622-9. PMid:16953352.

Miner NA, McDowell JW, Willcockson GW, Bruckner NI, Stark RL, Whitmore EJ. Antimicrobial and other properties of a new stabilized alkaline glutaraldehyde disinfectant/sterilizer. American Journal of Health-System Pharmacy. 1977 April 1; 34(4):376-82. https://doi.org/10.1093/ ajhp/34.4.376.

Vinay.P, Giridhar Reddy Y, Nikhilanand Hegde, Priyadarshini. Sterilization methods in orthodontics - A review. International Journal of Dental Clinics. 2011; 3(1):44-47.

gorman SP, Scott EM, Russell AD. Antimicrobial activity, uses and mechanism of action of Glutaraldehyde. J. Appl. Bacteriol. 1980 Apr; 48(2):161-90.

https://doi.org/10.1111/j.1365-2672.1980.tb01217.x. PMid: 6780502.

Shaffer MP, Belsito DV. Allergic contact dermatitis from glutaraldehyde in health-care workers. Contact Dermatitis. 2000 Sep; 43(3):150-56. https://doi.org/10.1034/j.16000536.2000.043003150.x. PMid:10985631.

Ioannou CJ, Hanlon GW, Denyer SP. Action of disinfectant quaternary ammonium compounds against Staphylococcus aureus. Antimicrob Agents Chemother. 2007 Jan; 51(1):296-306. https://doi.org/10.1128/AAC.00375-06. PMid: 17060529 PMCid: PMC1797692.

Tanner RS. Comparative testing and evaluation of hardsurface disinfectants. Journal of Industrial Microbiology. 1989; 4:145. https://doi.org/10.1007/BF01569799.

American Dental Association. Sterilization and Disinfection of dental instruments. https://www.ada.org/~/media/ADA/ Memb.

Rutala WA, Weber DJ. Disinfection and sterilization: An overview. Am. J. Infect. Control. 2013 May; 41(5 Suppl):S2-5. https://doi.org/10.1016/j.ajic.2012.11.005. PMid: 23622742.

Tina Chowdhary. Sterilization in orthodontics an insight. Heal Talk. 2013 November-December; 06(02):37-38.

Benyahia H, Merzouk N, EbnTouhami M, Zaoui F. Effects of sterilization and disinfection procedures on the corrosion of orthodontic ligature cutters. Int. Orthod. 2012 Mar; 10(1):1-15. https://doi.org/10.1016/j.ortho.2011.12.007. PMid: 22244107.

Akhilesh G Sukhlecha, ShuchitaVaya, Ghanshyam G Parmar, Chavda KD. Knowledge, attitude, and practice regarding sterilization among health-care staff in a tertiary hospital of western India. International Journal of Medical Science and Public Health. 2015; 4(10):1377-82. https://doi.org/10.5455/ijmsph.2015.20052015284.

Cash RG. Trends in sterilization and disinfection procedures in orthodontic offices. Am. J. Orthod. Dentofacial Orthop. 1990 Oct; 98(4):292-99. https://doi.org/10.1016/S08895406(05)81486-6.

Woo J, Anderson R, Maguire B, Gerbert B. Compliance with infection control procedures among California orthodontists. Am. J. Orthod. Dentofacial. Orthop. 1992 Jul; 102(1):68-75. https://doi.org/10.1016/08895406(92)70016-4.

Davis D, BeGole EA. Compliance with infection-control procedures among Illinois orthodontists. Am. J. Orthod. Dentofacial Orthop. 1998 Jun; 113(6):647-54.


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