Characterization of Lysinibacillus Sphaericus C3-41 Strain Isolated from Northern Karnataka, India that is Toxic to Mosquito Larvae


Abstract

One of the eco-friendly approaches to control mosquitoes is by the use of <i>Lysinibacillus sphaericus</i>. <i>L. sphaericus</i> (previous name <i>Bacillus sphaericus</i>) C3-41 (CP000817) was isolated from Rabbanahalli village of Yadgir district, Karnataka, India and was identified by biochemical and molecular means. Biochemical tests established its identity as <i>L. sphaericus</i>. It is also clear from the phylogenetic analysis that 16S ribosomal RNA nucleotide sequence of <i>L. sphaericus</i> C3-41 (CP000817) showed 99.73 per cent similarity with reference strain. Bioassay of <i>L. sphaericus</i> against second instar larvae of Culex quinquefasciatus recorded significantly higher mortality of 100 per cent at 0.075 % CSM. When assayed against second instar larvae of Anopheles subpictus mortality was 83.33 per cent at 0.075 % CSM.This new strain of L. sphaericus isolated from Northern dry zone of Karnataka, where the temperature ranges from 38°C to 46°C, could be a better biological pesticide to tackle the Culex mosquito and prevent diseases transmitted by it, particularly in Northern Karnataka.

Keywords

Lysinibacillus (Bacillus) Sphaericus, Biochemical Tests, 16s rRNA Gene Sequence, Culex Quin Quefasciatus, Bioassay

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