Potential of Biological Control Agents Against Pink Canker (Corticium Salmonicolor Berk.& Br.) in Apple


Eleven fungi of four genera, 5 bacteria and one actinomycete were isolated from pink (<i>Corticium salmonicolor Berk. and Br.</i>) canker of apple stems and twigs. Among these, <i>Trichoderma viride</i>, <i>T. harzianum</i>, <i>T. hamatum</i>, <i>T. koningi</i>, <i>Verticillium sp.</i>, <i>Penicillium sp.</i>, <i>Aspergillus versicolor</i>, and <i>actinomycete</i> were frequently encountered. In vitro evaluation of various microorganisms for their antagonistic activity revealed <i>T. koningi</i> was most effective in inhibiting the growth of pink canker (82.6 %) followed by <i>T. hamatum</i> (78.0 %) and <i>T. viride</i> (76.0%). <i>Pseudomonas sp.</i> (GenBank accession number: KF564924) provided maximum inhibition zone (15.70mm) followed by <i>Actinomycetes sp.</i> (14.2%). Among the bacterial isolates, BS1 (Kotgarh) isolate of <i>Bacillus subtilis</i> resulted maximum inhibition zone of 12.3 mm as compared to others. The per cent wound recovery was highest (48.5 %) in case of <i>T. koningi</i>, followed by <i>Actinomycetes sp.</i> (41.9 %) and <i>Pseudomonas sp.</i> (40.4 %) during two consecutive crop seasons (2011-2012).


Corticium Salmonicolor, Microflora, Antagonists, Wound Recovery, 18s DNA

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