Evaluation of Cry IIa transgenic chickpea lines for resistance to Helicoverpa armigera (Hubner) using detached leaf assay


  • Professor Jayashankar Telangana State Agricultural University (PJTSAU), Hyderabad, Telangana, 500030, India
  • Dr. Y S Parmar University of Horticulture and Forestry, Himachal Pradesh, 173230, India
  • Acharya N. G. Ranga Agricultural University, Guntur, Andhra Pradesh, 522509, India
  • International Crop Research for Semiarid Tropics (ICRISAT), Hyderabad, Telangana, 502324, India


Studies were conducted to evaluate transgenic chickpea lines encoding Cry IIa for resistance to Helicoverpa armigera. Significantly lower leaf damage was noticed in transgenic chickpea lines when compared to non-transgenic lines. Significant reduction in larval survival and weight gain were observed when H. armigera were fed on transgenic lines under laboratory conditions. Across the seasons (2011-12 and 2012-13), the transgenic chickpea lines BS5A.2(T2) 19-1P2 and BS5A.2(T2) 19-2P1 showed enhanced levels of resistance to H. armigera.


Cry IIa, Detached leaf assay, Helicoverpa armigera, Transgenic chickpea

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