Evaluation of carrier materials for formulating entomopathogenic fungus Lecanicillium Lecanii (Zimmermann) Zare and Gams

Jump To References Section

Authors

  • P. R. Nithya
     Department of Agricultural Entomology, College of Agriculture, Kerala Agricultural University, Vellayani, Trivandrum, Kerala ,IN
  • O. P. Reji Rani
     Department of Agricultural Entomology, College of Agriculture, Kerala Agricultural University, Vellayani, Trivandrum, Kerala ,IN

DOI:

https://doi.org/10.18311/jbc/2017/15556

Keywords:

Groundnut oil, Carrier, entomopathogenic fungus, Lecanicillium lecanii, sunflower oil

Abstract

Microbial pest control strategy is always challenged for its incompetent formulation technologies which hinder the action of potential microorganisms. Conidial viability is a key factor in formulating a microbial agent. Carrier material plays a pivotal role in maintaining the viability and virulence of an entomopathogenic fungus (EPF).  Evaluation studies using sunflower oil, groundnutoil and talc as the basic carrier materials and their enrichment with chitin and chitosan, was conducted at Collegeof Agricuture, Vellayani, Kerala. The ideal proportion of carrier material:technical ingredient and viability were assessed based on number of colony forming units (cfu) over a period of storage of 3 months. Chitin enriched ground nut oil (GNO + Chitin) and chitin enriched sunflower (SFO + chitin) were observed as the best carriers for formulating the fungus.  Ideal proportion of carrier: technical ingredient in oil formulations was found to be 65:35 per cent.  The number of cfu observed with chitin enriched GNO and SFO by the end of three months of storage was 2.27 x 106 ml-1 and 2.20 x 106 ml-1 under room temperature respectively. Enrichment of oil formulations (GNO and SFO) with chitin could thus sustain viability as well as spore count of L. lecanii till the end of experimental period (three months). 

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biography

P. R. Nithya, Department of Agricultural Entomology, College of Agriculture, Kerala Agricultural University, Vellayani, Trivandrum, Kerala

Agricultural Entomology, PG scholar

Downloads

Published

2017-09-12

How to Cite

Nithya, P. R., & Reji Rani, O. P. (2017). Evaluation of carrier materials for formulating entomopathogenic fungus <I>Lecanicillium Lecanii</I> (Zimmermann) Zare and Gams. Journal of Biological Control, 31(1), 50–55. https://doi.org/10.18311/jbc/2017/15556

Issue

Volume 31, Issue 1, March 2017

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC

 

References

Abdel-Kader MM, El-Mougy NS, Aly MDE, Lashin SM. 2012. Long activity of stored formulated bio-agents against some soil-borne plant pathogenic fungi causing root rot of some vegetables. J Appl Sci Res. 8(4): 1882–1892.

Alves RT, Bateman RP, Gunn J, Prior C, Leather SR. 2002. Effects of Different Formulations on Viability and Medium-Term Storage of Metarhizium anisopliae Conidia. Neotrop Entomol. 31(1): 91–99.

Banu JG, Gopalakrishnan N. 2012. Development of formulations of a native entomopathogenic fungus, Lecanicillium lecanii and testing virulence against mealybug, Paracoccus marginatus infesting cotton. Indian J Plant Prot. 40(3): 182–186.

Banu JG. 2013. Effect of different storage conditions on spore viability of Lecanicillium lecanii formulations and infectivity to mealybug, Paracoccus marginatus. Int J Plant Prot. 6(2): 334–337.

Batta YA. 2003. Production and testing of novel formulations of the entomopathogenic fungus Metarhizium anisopliae (Metschinkoff) Sorokin (Deuteromycotina: Hyphomycetes). Crop Prot. 22: 415–422.

Batta YA, Rahman M, Powis K, Baker G, Schmidt O. 2011. Formulation and application of the entomopathogenic fungus: Zoophthora radicans (Brefeld) Batko (Zygomycetes: Entomophthorales). J Appl Microbiol. 110: 831–839.

Cuthbertson AGS, Walters KFA, Northing P. 2005. The susceptibility of immature stages of Bemisia tabaci (G.) to the entomopathogenic fungus Lecanicillum muscarium (V. lecanii (Zimm.) on tomato and verbena foliage. Mycopathologia 159: 23–29.

Derakhshan A, Rabindra RJ, Ramanujam B. 2008. Effect of storage of formulations on viability of Verticillium lecanii(Zimmermann) Viegas and its virulence to Brevicorne brassicae(L). J Biol Sci. 8: 498–501.

Diaz BM, Oggerin M, Lastra CCL, Rubio V, Fereres A. 2009. Characterization and virulence of Lecanicillium lecanii against different aphid species. Biocontrol 54(6): 825–835.

Gerding-Gonzalez M, France A, Sepulveda ME, Campos J. 2007. Use of chitin to improve a Beauveria bassiana alginate-pellet formulation. Biocontrol Sci Technol. 17(1): 105–110.

Hall RA, Papierok B. 1982. Fungi as biocontrol agents of arthropods of agricultural and medical importance. Parasitology 84: 205–240.

Hegde SV. 2002. Liquid biofertilizers in Indian agriculture. Biofertilizer News Lett. 12: 17–22.

Kim JJ, Goettel MS, Gillespie DR. 2007. Potential of Lecanicillium species for dual control of aphids and the cucumber powdery mildew fungus, Sphaerotheca fuliginea. Biol Control 40: 327–332.

Knudsen GR, Johnson JB, Eschen DJ. 1990. Alginate pellet formulation of a Beauveria bassiana (Fungi: Hyphomycetes) isolate pathogenic to cereal aphids. J Econ Entomol. 83: 2225–2228.

Lomer CH, Lomer CJ. 2001. Collection of Insect Pathogens. Lubilosa Tech Bull. 2: 24p.

Nirmala R, Ramanujam B, Rabindra RJ, Rao NS. 2006. Effect of entomofungal pathogens on mortality of three aphid species. J Biol Control 20: 89–94.

Park H, Kim K. 2010. Selection of Lecanicillium strains with high virulence against developmental stages of Bemisia tabaci. Mycobiology 38: 210–214.

Pavlyushin VA, Tyuterev SL, Novikova II, Popova EV, Bykova GA, Boikova IV, Khatskevich LK. 2005. New preparations for combined protection of plants against diseases of various etiology. Russ Agric Sci. 12: 7–12.

Pindi PK, Satyanarayana SDV. 2012. Liquid Microbial Consortium: A Potential Tool for Sustainable Soil Health. J Biofertil Biopestici. [e-journal] 3(4). Available from: http://www.omicsonline.org/liquid-microbial-consortium-a-potential-tool-for-sustainable-soil-health.pdf. ISSN 2155-6202.

Prior C, Jollands P, Patourel GL. 1988. Pathogenicity test of Beauveria bassiana (Balsamo) against oil palm bagworm (Metisaplana Wlk). Elaeis 5(2): 92–101.

Roberts WK, Selitrennikoff CP. 1988. Plant and bacterial chitinases antifungal activity. J Gen Microbiol. 134: 169–176.

Sriram S, Palanna KB, Ramanujam B. 2010. Effect of chitin on the shelf- life of Trichoderma harzianum in talc formulation. Indian J Agric Sci. 80(10): 80–82.

Ujjan AA, Shahzad S. 2012. Use of entomopathogenic fungi for the control of mustard aphid (Lipaphis erysimi) on canola (Brassica napus L). Pak J Bot. 44(6): 2081–2086.

Verhaar MA, Hijwegen T, Zadoks JC. 1999. Improvement of the efficacy of Verticillium lecanii used in biocontrol of Sphaerotheca fuliginea by addition of oil formulations. BioControl 44: 73–87.

Vimaladevi PS, Prasad YG, Chowdary A. 2002. Effect of drying and formulation of conidia on virulence of the entomofungal pathogen, Nomuraea rileyi (Farlow) Samson. J Biol Control 16(1): 43–48.

Wenzel IM, Filho BA. 2011. Effect of pesticides on the pathogenicity of conidia of Lecanicillium lecanii on Tetranychus urticae. Arq Inst Biol. 78(2): 261–266.