Biocontrol and growth promotive potential of Streptomyces spp. in black pepper (Piper nigrum L.)

Authors

  • Division of Crop Protection, ICAR Indian Institute of Spices Research, Kozhikode 6730112, Kerala
  • Division of Crop Protection, ICAR Indian Institute of Spices Research, Kozhikode 6730112, Kerala
  • Division of Crop Protection, ICAR Indian Institute of Spices Research, Kozhikode 6730112, Kerala
  • Division of Crop Protection, ICAR Indian Institute of Spices Research, Kozhikode 6730112, Kerala
  • Division of Crop Protection, ICAR Indian Institute of Spices Research, Kozhikode 6730112, Kerala

DOI:

https://doi.org/10.18311/jbc/2016/15592

Keywords:

Black pepper biocontrol, consortia, growth promotion, IAA production, PGPR, Streptomyces spp., Phytophthora capsici

Abstract

Actinomycetes isolated from the rhizosphere of black pepper and from vermicompost were tested for their antagonistic effect against Phytophthora capsici and Radopholus similis, the causal agents of foot rot and slow decline diseases of black pepper. Based on in vitro evaluations, four isolates were shortlisted (IISR Act2, IISR Act5, IISR Act6, and IISR Act9) and subjected to in vivo evaluation for Phytophthora infection by challenge inoculation and also greenhouse evaluation for growth promotion in black pepper. Rooted plants of black pepper were raised in soil amended with Actinomycetes strains individually and in combinations in portray and were transplanted into earthenware pots containing potting mixture amended with respective actinomycetes keeping un-amended plants as control. Observations were recorded on growth parameters like plant height, root weight, shoot weight and root infection by nematodes. Besides, soil was also analyzed for pH, dehydrogenase activity, EC and NPK content to know the influence of actinomycetes on soil microflora as well as on nutrient status. The results showed that consortia are more effective than individual isolates. Consortia holding IISR Act5+IISR Act9 were found highly effective in enhancing all the growth parameters followed by IISR Act2+ IISR Act9 and IISR Act2 + IISR Act5. The dehydrogenase activity was found higher in these consortia showing the higher microbial metabolic activity. Root lesions were also negligible in these treatments. Being effective in growth promotion as well as antagonistic activity, the isolates were tested for plant growth promotion and biocontrol traits. Among the isolates, IISR Act9 was found highly efficient in IAA production (119μg/ml) when compared to IISR Act2 (36.25μg/ml) and IISR Act5 (32.4μg/ml). Hence based on the growth promotive and pathogen suppressive effect, the consortia of either IISR Act5+IISR Act9 , IISR Act2+IISR Act9 or IISR Act2+IISR Act5 can be effectively used in black pepper for growth promotion and biological control of foot rot and slow decline diseases. The potential actinomycetes were identified as Streptomyces spp. as per Bergey's manual and rpoB gene sequence similarity of which IISR Act2 is identified as Streptomyces sp., IISR Act9 as Streptomyces albus and IISR Act5 as Streptomyces sp.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Aldesuquy HS, Mansour FA, Abo-Hamed SA. 1998. Effect of the culture filtrate of Streptomyces on growth and productivity of wheat plants. Folia Microbiol. 43: 465–470.

Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389–402.

Bano N, Musarrat J. 2003. Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent. Current Microbiol. 46: 324–328.

Basak BB, Biswas DR. 2009. Influence of potassium solubilizing microorganisms (Bacillus mucilaginous) and waste mica on potassium uptake dynamics by sudan grass (Sorghum vulgare Pers.) grown under tow Alfisols. Pl Soil 317: 235–255

Nanjwade BK, Chandrashekhara S, Goudanavar PS, Shamarez AM, Manvi FV. 2010. Production of antibiotics from soil-isolated Actinomycetes and evaluation of their antimicrobial activities. Tropical J Pharmaceutical Res. 9: 373–377.

Behal V. 2000. Bioactive products from Streptomyces. Adv Appl Microbiol. 47: 113–157

Brown M. 1972. Plant growth substances produced by micro-organisms of soil and rhizosphere. J Appl Bacteriol. 35: 443–451

Devasahayam S, John Zachariah T, Jayashree E, Kandiannan K, Prasath D, Santhosh J Eapen, Sasikumar B, Srinivasan V, Suseela Bhai R. 2015. Thomas L, Rajeev P(Eds). Black pepper - Extension pamphlett. ICAR-Indian Institute of Spices Research, Kozhikode, Kerala.

Casida LE. 1977. Microbial metabolic activity in soil as measured by dehydrogenase determinations. Appl Environ Microbiol. 34: 630–636.

Doumbou CL, Akimov V, Beaulieu C. 1998. Selection and characterization of microorganisms utilizing thaxtomin A, a phytotoxin produced by Streptomyces scabies. Appl Environ Microbiol. 44: 4313–4316.

Doumbou CL, Salove MK, Crawford DL, Beaulieu C. 2001. Actinomycetes, promising tools to control plant diseases and to promote plant growth. Phytoprotection 82: 85–102.

El-Tarabily KA. 2008. Rhizosphere-competent isolates of streptomycete and non-streptomycete actinomycetes capable of producing cell-wall-degrading enzymes to control Pythium aphanidermatum damping-off disease of cucumber. Can J Bot. 84: 211–222.

El-Abyad MS, El-Sayed MA, El-Shanshoury AR, El-Sabbagh SM. 1993. Towards the biological control of fungal and bacterial diseases of tomato using antagonism Streptomyces spp. Pl Soil 149: 85–195.

El-Tarabilya KA, Sivasithamparam K. 2006. Non-streptomycete actinomycetes as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters. Soil Biol Biochem. 38: 1505–1520.

Fenton AM, Stephens PM, Crowley J. O'Callaghan M, O'Gara F. 1992. Exploiting gene(s) involved in 2, 4-diacetylphloroglucinol biosynthesis in order to improve the biocontrol ability of a pseudomonad strain. Appl Environ Microbiol. 58: 3873–3878.

Glick BR. 1995. The enhancement of plant growth by free-living bacteria. Can J Microbiol. 41: 109–117.

Good Fellow M, Simpson KE 1987. Ecology of Streptomycetes. Frontiers Appl Microbiol. 2: 97–125.

Gopalakrishnan S, Kiran BK, Humayun P, Vidya MS, Deepthi K and Rupela O. 2011. Biocontrol of charcoal-rot of sorghum by actinomycetes isolated from herbal vermicompost. African J Biotechnol. 10: 18142–18152.

Gopalakrishnan S, Humayun P, Vadlamudi S, Vijayabharathi R, Bhimineni RK, Om R. 2012. Plant growth-promoting traits of Streptomyces with biocontrol potential isolated from herbal vermicompost. Biocontrol Sci Technol. 22: 1199–1210.

Hao D, Gao P, Liu P, Zhao J, Wang Y, Yang W, Lu Y, Shi T, Zhang X. 2011. AC3-33, a novel secretory protein, inhibits Elk1 transcriptional activity via ERK pathway. Mol Boil Rep. 38: 1375–1382.

Jeffrey LSH. 2008. Isolation, Characterization and identification of actinomycetes from agriculture soils at Semongok, Sarawak. Afr J Biotechnol. 7: 3697–3702.

Jolanta S, Joanna Z, Magdalena P, Aleksandra R. 2012. Biologically active secondary metabolites from Actinomycetes. Cent Eur J Biol. 7: 373–390.

Katiyar V, Goel R. 2004. Siderophore-mediated plant growth promotion at low temperature by mutant of fluorescent pseudomonad. Plant Growth Regul. 42: 239–244.

Khamna S, Yokota A, Peberdy JF, Lumyong S. 2010. Indole-3- Acetic production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils. Eurasia J BioSci. 4: 23–32.

Kutchma AJ, Roberts MA, Knaebel DB, Crawford DL. 1998. Small-scale isolation of genomic DNA from Streptomyces mycelia or spores. Bio Techniques 24: 452–456.

Merckx R, Dijkra A, Hartog AD, Veen JAV. 1987. Production of root-derived material and associated microbial growth in soil at different nutrient levels. Biol Fertility Soils 5: 126–132.

Merriman PR, Price RD, Kollmorgen JF, Piggott T, Ridge EH. 1974. Effect of seed inoculation with Bacillus subtilis and Streptomyces griseus on the growth of cereals and carrots. Aust J Agric Res. 25: 219–226.

Muller G, Raymond KN. 1984. Specificity and mechanism of ferrioxamine mediated iron transport in Streptomyces pilosus. J Bacteriol. 160: 304–312.

Muller G, Matzanke BF, Raymond KN. 1984. Iron transport in Streptomyces pilosus mediated by ferrichrome siderophores, rhodotorulic acid, and enantio-rhodotorulic acid. J Bacteriol. 160: 313–318.

Panhwar QA, Othman R, Rahman ZA, Meon S, Ismail MR. 2012. Isolation and characterization of phosphate-solubilizing bacteria from aerobic rice. Afr J Biotechnol. 11: 2711–2719.

Patten C, Glick BR. 2002. Role of Pseudomonas putida in indole acetic acid in development of the host plant root system. Appl Env Microbiol. 68: 3795–3801.

Prashith TR, Shoba KS, Onkarappa. 2010. Fascinating diversity and potent biological activities of Actinomycetes metabolites. J Pharmacy Res. 3: 250–256

Pornthip R, Hartmut L, Nuchanart T, Saisamorn L. 2011. Nematicidal activity of fervenulin isolated from a nematicidal actinomycete, Streptomyces sp. CMU-MH021, on Meloidogyne incognita. World J Microbiol Biotechnol. 27: 1373–1380.

Zeng Q, Huang H, Zhu J, Fang Z, Sun Q, Bao S. 2013. A new nematicidal compound produced by Streptomyces albogriseolus HA10002. Antonie Van Leeuwenhoek. 2103(5): 1107–1111.

Schwyn B, Neilands JB. 1987. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 160: 47–56.

Smith JL, Doran JW. 1996. Measurement and use of pH and electrical conductivity for soil quality analysis. P. 169-185. In: Doran JW, Jones AJ. (Eds.). Methods for assessing soil quality. Soil Science Society of America Spec. Publ. 49. SSSA, Madison, WI.

Khamna S, Yokota A, Peberdy JF, Lumyong S. 2009. Antifungal activity of Streptomyces spp. isolated from rhizosphere of Thai medicinal plants. Int J Integr Biol. 6: 144–149.

Suzuki S, Yamamoto K, Okuda T, Nishio M, Nakanishi N, Komatsubara S. 2000. Selective isolation and distribution of Actinomadura rugatobispora strains in soil. Actinomycetologica 14: 27–33.

Takizawa M, Colwell RR, Hill RT. 1993. Isolation and diversity of actinomycetes in the Chesapeake Bay. Appl Envl Microbiol. 59: 997–1002.

Tsavkelova EA, Klimova SYu, Cherdyntseva TA, Netrusov AI. 2006. Microbial producers of plant growth stimulators and their practical use: a review. Appl Biochem Microbiol. 42: 117–126.

Weed SB, Davey CB, Cook MG. 1969. Weathering of mica by fungi. Soil Sci Soc Am J. 33: 702–706.

Downloads

Published

2017-03-16

How to Cite

Suseela Bhai, R., Lijina, A., Prameela, T. P., Krishna, P. B., & Thampi, A. (2017). Biocontrol and growth promotive potential of <i>Streptomyces</i> spp. in black pepper (<i>Piper nigrum</i> L.). Journal of Biological Control, 30(3), 177–189. https://doi.org/10.18311/jbc/2016/15592

Issue

Section

Research Articles