Cross infection of Pieris brassicae granulosis virus on other siblings of Pieris species


Affiliations

  • Manipur University, CAS in Life Sciences, Imphal, Manipur, 795003, India
  • Manipur University, CAS in Life Sciences, Manipur University, Imphal, Manipur, 795003, India
  • ICAR-National Bureau of Agricultural Insect Resources, Division of Germplasm Collection & Characterization, Bengaluru, Karnataka, 560024, India

Abstract

Pieris brassicae granulosis virus (PibrGV) has been found to infect yet another two siblings namely P. canidia Linn. & Pontia daplidice (=Pieris daplidice) (Roeber) in addition to P. brassicae (Linn.). The susceptibility of P. canidia and P. daplidice has been established clearly in this work by inoculating them with PibrGV @ 5x104 occlusion bodies (OBs) per ml by the conventional leaf disc method. All the inoculated larvae exhibited typical symptoms of viral infection after 5-7 days of post infection. The above three species of the genus Pieris occur on cabbage and cauliflower plants at different periods in the valley region of Imphal and therefore, it is possible to control all the three species of Pieris using the same virus. LC50 value of PibrGV on P. brassicae, P. canidia and P. daplidice was found to be 7.9x104; 10x106; and 6.3x106 OBs/ml and the LT50 was 134.89; 138.03; 174.50 hours, respectively.


Keywords

Cross infection, granulosis virus, Pieris canidia, Pieris brassicae, Pontia daplidice

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References

Arun D, Singh, KI, Gupta, MK, Sobitadevi, P. 2017. Biorational management of major lepidopterous pests and their influence on yield of cabbage crop under Manipur Valley. J Entomol Zool Stud. 5: 1546-1551.

Ashwini Ananda AK, Bijaya Devi, Singh A, Barik S, Bairwa MK. 2017. Cabbage (Brassica oleracea l. var. capitata) cv. Rareball introduction with knol khol and broad bean intercropping: yield efficiency under foot hills of Imphal-west. Pharma Innovation J. 6: 339-341.

Bijaya P. 1998. Bioecology of Myzus persicae (Sulzer) (Homoptera: Aphididae) on certain cole crops of Manipur, Ph D thesis, Manipur University, India, 189 pp.

DAC. 2017. Govt. of India Horticulture Statistics at a glance. 197.

Charles C, Tatchell M, Williams CF. 1981. The comparative susceptibilities of Pieris brassicae and Pieris rapae to a granulosis virus from Pieris brassicae. J Invertebr Pathol. 38: 273 – 280. https://doi.org/10.1016/00222011(81)90133-6

Crook NE. 1981. A comparison of the granulosis virus from Pieris brassicae and Pieris rapae. Virology 115: 173181. https://doi.org/10.1016/0042-6822(81)90099-4 Devjani P. 1999. Bio-ecology and control of insect pests of cauliflower in Manipur. Ph.D thesis, Manipur University, India, 189 pp.

Entwistle PF. 1998. World survey of virus control of insect pest- People of Republic China, In: pp. 258-268, Hunter FR, Fujita, Entwistle PF, Evans H Crook NE (Eds.). Insect Virus and pest management. John Willy and Sons, New York, USA.

Finney D. 1977. Probit Analysis. Cambridge University Press, London, UK, 333 PP.

NIFTEM IFTEM & MOFPI (2012-13): National Horticulture Production Database-2012-13, MOA & Ministry of Food Processing Industries, GOI.

Rabindra RJ, Rajasekaran B, Jayaraj S. 1997. Combined action of NPV and neem bitter against Spodoptera litura (Fab.) larvae. J Biol Control 11: 5-9.

Sangeeta W. 2011. Studies on the virus infecting the cabbage butterfly, Pieris brassicae (Linn.) (Lepidoptera). Ph. D thesis, Manipur University, India, 187 pp.

Sangeeta W, Varatharajan R. 2011. Granulosis virus of the cabbage pest, Pieris brassicae – a potential biopesticide for the control of Pieris spp. (Pieridae: Lepidoptera). In: Pp 12. Ambrose DP (Ed.). Insect Pest Management, A Current Scenario. St. Xavier College, Palayamkottai, Tirunelveli, Tamil Nadu, India.

Sangeeta W, Ingobi M, Dhanapati K, Pandey RR, Varatharajan R. 2009. Pathogenecity of Pieris brassicae granulosis virus infecting the caterpillars of P. brassicae (Lepidoptera: Pieridae). In: Pp. 56-61. Ignacimuthu S and David BV (Eds.). Ecofriendly Insect Pest Management. Elite Publishing House Pvt. Ltd., New Delhi, India.

Smith KM. 1976. Virus – Insect relationships. Longman Group Limited, 291 pp.

Shah BH, Zethner O, Gul H, Chaudhry MI. 1979. Control experiments using Agrotis segetum granulosis virus against Agrotis ipsilon (Lepidoptera: Noctuidae) on tobacco seedlings in northern Pakistan. Entomophaga 24: 393- 401. https://doi.org/10.1007/BF02374178

Varatharajan R. 2008. Augmentation and evaluation of PbGV viral pesticide for the control of the cabbage butterfly, Pieris brassicae (L.). DBT Project Final Report, New Delhi, India, 45 pp.

Zethner O, Khan BM, Ismail M, Chaudhry MI, Bolet B, Khan S, Khan HU, Gul H, Qgaard L, Zaman M, Gul Nawaz. 1987. Agrotis segetum granulosis virus as a control agent against field populations of Agrotis ipsilon and A. segetum (Lepidoptera: Noctuidae) on tobacco, okra, potato and sugar beet in Northern Pakistan.

Entomophaga 32: 449-455. https://doi.org/10.1007/BF02373513


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