Bio-efficacy of different biological control agents for the management of chilli fruit rot/anthracnose disease
Chilli (Capsicum annum L.) an important economic crop worldwide is severely infected by fruit rot disease which may cause yield losses of up to 100%. Although different chemical fungicides are being recommended and used for the management of the disease, biocontrol-based strategy attracts considerable attention and offers great potential of novel biocontrol agents. Further, biological control methods for chilli fruit rot/anthracnose disease have not received much attention. Therefore, an investigation was carried out to assess the efficacy of different biological control agents against chilli fruit rot/anthracnose disease. Antagonistic yeast isolates Pichia guillermondii (Y-12), Hanseniaspora uvarum (Y-73) and Trichoderma asperellum (Th-3), Pseudomonas fluorescens (Pf-1) were tested through seed treatment, seedling dip and foliar spray at concentration of 2 × 108 cfu/g. The pathogens viz., Colletotrichum capsici, Alternaria alternata and Periconia byssoides were found associated with fruit rot during the study. Lowest mean disease intensity (MDI), highest disease control (DC) over untreated control was recorded in the treatment <i>P. guillermondii</i> (Y-12) (5.39% MDI, 64.72% DC) and this was found at par with the treatment <i>P. fluorescens</i> (Pf-1) (5.92% MDI, 65.52% DC). However, <i>T. asperellum</i> (Th-3) showed satisfactory results. The present study highlights the efficacy of antagonistic yeast species against chilli fruit rot pathogens and these findings serve as base for further exploration and exploitation of yeast species for eco-friendly management of crop diseases.
Anonymous. 2018. Chilli Outlook, February, Agricultural Market Intelligence Centre, PJTSAU. pp. 1-5.
Ashwini N, Srividya S. 2014. Potentiality of Bacillus subtilis as biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum gloeosporioides OGC1. 3Biotech 4: 127-136.
Basak AB, Fakir GA, Mridha MAU. 1994. Studies on the prevalence of six major fruit rot diseases of chilli at different stages of fruit development in Chittagong district. Chittagong Uni Stud Sci. 18: 125-128.
Boonratkwang C, Chamswarng C, Intanoo W, Juntharasri V. 2007. Effect of secondary metabolites from Trichoderma harzianum strain pm9 on growth inhibition of Colletotrichum gloeosporioides and chilli anthracnose control, pp. 232-336. Proceeding of the 8th National Plant Protection Conference, Phisanulok:Naresuan University, Chang Wat Phitsanulok 65000, Thailand.
Chakraborty BN, Chakraborty U, Saha A, Dey PL, Sunar K. 2010. Molecular characterization of Trichoderma viride and Trichoderma harzianum isolated from soils of North Bengal based on rDNA markers and analysis of their PCR-RAPD profiles. Global J Biotech Biochem. 5(1): 55-61.
Chanchaichaovivat A, Ruenwongsa P, Panijpan B. 2007. Screening and identification of yeast strains from fruit and vegetables: potential for biological control of postharvest chilli anthracnose (Colletotrichum capsici). Biol Control 42: 326-335. https://doi.org/10.1016/j.biocontrol.2007.05.016
Ferdousi Begum M, Rahman MA, Firoz Alam M. 2010. Biological control of Alternaria fruit rot of chilli by Trichoderma species under field condition. Mycobiology 38: 113-117. https://doi.org/10.4489/MYCO.2010.38.2.113 PMid:23956637 PMCid: PMC3741560
Guo-yin T, Zgi-Ling Y, Zhi-lin Y, Shou-an S. 2013. Morphological, molecular and pathologenic characterization of Alternaria longipes, the fungal pathogen causing leaf spot on Atractylodes macrocephala. African J Microbiol Res. 7: 2589-2595. https://doi.org/10.5897/AJMR12.2091
Hebert PDN, Cywinska A, Ball SL, DeWaard JR. 2003. Biological identifications through DNA barcodes. Proc Royal Soc London Series B-Biol Sci. 270: 313–321. https://doi.org/10.1098/rspb.2002.2218 PMid:12614582 PMCid:PMC1691236
Intanoo W, Chamswarng C. 2007. Effect of antagonistic bacterial formulations for control of anthracnose on chilli Fruits, pp. 309-322. Proceeding of the 8th National Plant Protection Conference, Phisanulok:Naresuan University, Chang Wat Phitsanulok 65000, Thailand.
Jaihan P, Sangdee K, Sangdee A. 2016. Selection of entomopathogenic fungus for biological control of chili anthracnose disease caused by Colletotrichum spp. European J Plant Pathol. 146: 551–564.
Jeyalakshmi C, Seetharaman K. 1998. Biological controls of fruit rot and die-back of chilli with plant products and antagonistic microorganisms. Plant Dis Res. 13: 46-48.
Lenné JM, Parbery DG. 1976. Phyllosphere antagonists and appressoria formation in Colletotrichum gloeosporioides. Tranc Brit Mycol Soc. 66: 334–336. https://doi.org/10.1016/S0007-1536(76)80065-4
Maymon M, Barbul DMO, Zveibil A, Elad Y, Freeman S. 2004. Identification of Trichoderma biocontrol isolates to clades according to ap-PCR and ITS sequence analyses. Phytoparasi. 32: 370-375. https://doi.
Nagrale DT, Gaikwad AP, Sharma L. 2013. Morphological and cultural characterization of Alternaria alternata (Fr.) Keissler blight of gerbera (Gerbera jamesonii H. Bolus ex J.D. Hook). J Appl Nat Sci. 5: 171-178.
Pakdeevaraporn P, Wasee S, Taylor PWJ, Mongkolporn O. 2005. Inheritance of resistance to anthracnose caused by Colletotrichum capsici in capsicum. Pl Breed. 124: 206–208. https://doi.org/10.1111/j.14390523.2004.01065.x
Poonpolgul S, Kumphai S. 2007. Chilli pepper anthracnose in Thailand: Country report. pp.23. In: Oh, DG and K. T. Kim, KT (Eds.). Abstracts of the First International Symposium on Chilli Anthracnose. National
Horticultural Research Institute: Rural Development of Administration, Republic of Korea.
Ramachandran N, Rathnamma K. 2006. Colletotrichum acutatum - a new addition to the species of chilli anthracnose pathogen in India. Paper Presented at the Annual Meeting and Symposium of Indian Phytopathological Society, Central Plantation Crops Research Institute, Kasaragod, Kerala, India.
Rosa Magri MM, Tauk-Tornisielo SM, Ceccato Antonini SR. 2011. Bioprospection of yeasts as biocontrol agents against phytopathogenic molds. Braz Arch Biol Technol. 54: 1-5.https://doi.org/10.1590/S151689132011000100001
Saxena A, Raghuwanshi R, Gupta VK, Singh HB. 2016. Chilli anthracnose: the epidemiology and management. Front Microbiol. 7: 1527. https://doi.org/10.3389/ fmicb.2016.01527 PMid:27746765 PMCid:PMC5044472
Simmons EG. 2007. Alternaria. An Identification Manual. CBS Biodiversity Series No. 6. CBS Fungal Biodiversity Centre, Utrecht, the Netherlands, 775 pp.
Singh HB, Singh BN, Singh SP, Sarma BK. 2012. Exploring different avenues of Trichoderma as a potent biofungicidal and plant growth promoting candidate – an over view. Rev Plant Pathol. 5: 315–426.
Subhani MN. 2015. Isolation and efficacy of fungicides and homeo-fungicides against anthracnose of chilies caused by Colletotrichum capsici. Pakistan J Nutri. 14: 325-329. https://doi.org/10.3923/pjn.2015.325.329
Torres-Calzada C, Tapia-Tussell R, Quijano-Ramayo A, Martin-Mex R, Perez-Brito D. 2011. A species-specific polymerase chain reaction assay for rapid and sensitive detection of Colletotrichum capsici. Mol Biotechnol. 49: 48-55. https://doi.org/10.1007/s12033-011-9377-7 PMid:21253896
Tutte J. 1969. Plant pathological methods fungi and bacteria. Burgess Publishing Company, USA, 229 pp.
Vasanthakumari MM, Shivanna MB. 2013. Biological control of anthracnose of chilli with rhizosphere and rhizoplane fungal isolates from grasses. Arch Phyto Pathol. 46: 1641–1666.
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.