A chemically sulfated derivative galactomannan from Adenanthera pavonina seeds elicits defense-related responses in cowpea and confers protection against Colletotrichum gloeosporioides

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Authors

  • Anna L. N. Varela
     Department of Biochemistry, Science Center, Federal University of Ceara, Fortaleza- CE, 60020-181 ,BR
  • Ilka M. Vasconcelos
     Department of Biochemistry, Science Center, Federal University of Ceara, Fortaleza- CE, 60020-181 ,BR
  • Rodolpho G. G. Silva
     Department of Biochemistry, Science Center, Federal University of Ceara, Fortaleza- CE, 60020-181 ,BR
  • Hudson F. N. Moura
     Department of Biochemistry, Science Center, Federal University of Ceara, Fortaleza- CE, 60020-181 ,BR
  • Thiago F. Martins
     Department of Biochemistry, Science Center, Federal University of Ceara, Fortaleza- CE, 60020-181 ,BR
  • Darcy M. F. Gondim
     University of Fortaleza (UNIFOR), Fortaleza, Ceara ,BR
  • Nagila M. P. S. Ricardo
     Department of Chemistry, Science Center, Federal University of Ceara, Fortaleza- CE, 60020-181 ,BR
  • Francisco C. O. Freire
     Brazilian Enterprise for Agricultural Research (EMBRAPA-CNPAT), Fortaleza, Ceara ,BR
  • Jose T. A. Oliveira
     Department of Biochemistry, Science Center, Federal University of Ceara, Fortaleza- CE, 60020-181 ,BR ORCID logo http://orcid.org/0000-0003-1207-1140

DOI:

https://doi.org/10.18311/jbc/2019/23391

Keywords:

Colletotrichum gloeosporioides cowpea, defense response, sulfated galactomannan, Vigna unguiculata
Colletotrichum gloeosporioides

Abstract

This work was conducted to assess whether or not a natural occurring polysaccharide from Adenanthera pavonina seeds (PLSAp) and its chemically sulfated derivative galactomannan (SPLSAp), which did not act as fungicides, induce defense proteins and protect a susceptible cowpea (Vigna unguiculata) genotype (BR3-Tracuateua) against the phytopathogenic fungus Colletotrichum gloeosporioides. Twelve-day-old plants were sprayed to run-off with PLSAp or SPLSAp, both at 100 and 200 mg L-1, dissolved in 0.01% Triton X-100, which served as control. The primary leaves were collected at 0, 6, 12, 24, and 48 h after the carbohydrate treatments and the activities of guaiacol peroxidase (POX), β-1,3-glucanase (βGLU), and chitinase (CHI) were determined to verify the ability of the studied galactomannans to induce alterations in their kinetics. In addition, the primary leaves of a plant group previously sprayed with SPLSAp and 6 h later inoculated with C. gloeosporioides were collected at 0, 6, 12, 24, and 48 h after the polysaccharide treatment, the enzyme activities measured, and the severity of the fungal disease evaluated. SPLSAp induced more prominently POX, βGLU, and CHI activity than PLSAp. Importantly, the combined treatment of SPLSAp and C. gloeosporioides was even more effective as enzyme inductor than SPLSAp alone, and the necrotic lesions of the anthracnose disease caused by the fungus were much less severe compared to control plants. The results indicate that SPLSAp induces plant defense proteins and increases the cowpea tolerance to C. gloeosporioides. These findings indicate that SPLSAp merits further investigation as a promising alternative or a supplemental environmentally friendly protector agent against anthracnose.

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Published

2019-05-24

How to Cite

Varela, A. L. N., Vasconcelos, I. M., Silva, R. G. G., Moura, H. F. N., Martins, T. F., Gondim, D. M. F., Ricardo, N. M. P. S., Freire, F. C. O., & Oliveira, J. T. A. (2019). A chemically sulfated derivative galactomannan from <i>Adenanthera pavonina</i> seeds elicits defense-related responses in cowpea and confers protection against <i>Colletotrichum gloeosporioides</i>. Journal of Biological Control, 33(1), 7–17. https://doi.org/10.18311/jbc/2019/23391

Issue

Volume 33, Issue 1, March 2019

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2019-03-08
Accepted 2019-04-16
Published 2019-05-24

 

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