Molecular Characterization of Bacillus isolates with Insecticidal Activity against Greater Wax Moth, Galleria mellonella L. (Pyralidae: Lepidoptera)
Six local Bacillus strains isolated from soils from different parts of Karnataka were characterized by their high toxicity against third instar larvae of greater wax moth (Galleria mellonella L.). The PCR amplification analysis using B. thuringiensis specific primers revealed that only four isolates are Bt isolates. The PCR amplification analysis for cry genes indicated the presence of amplified fragments characteristic of three different cry genes cry1Aa, cry2Aa and cry11Aa. Results showed the presence of cry11Aa and cry1Aa in isolate N12 with expected sizes of 816bp and 891-1089bp, respectively. Bt isolate; A-7 harbored cry1Aa, cry2Aa and cry11Aa with product size 891-1308, 235-696 and 636bp, respectively. While the isolates M-2 and A3 contained only one gene cry2Aa with a product size 696-735bp. The two isolates; N-12 and A-7 were harbored multiple cry genes. The cry2Aa genes were the most frequently found among the three local Bt isolates. The profiles of cry genes indicated that all isolates were active against lepidopteran insects, and therefore these strains could be potential candidates in the search for biocontrol agents in management of greater wax moth, G. mellonella with specific lepidopteran activity.
Galleria mellonella, Bacillus thuringiensis, PCR, cry genes, Lepidoptera.
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