Characterization and Evaluation of Native Pseudomonas Spp. of South Gujarat against Leaf Blast in Finger Millet
Seven Pseudomonas isolates were isolated from different locations of south Gujarat. Based on the morphological, biochemical and physiological characterization of these isolates, four bacterial isolates were identified as Pseudomonas aeruginosa (PaWP, PaWS, PaRS and PaNS) and three as P. fluorescens (PfWN, PfRB and PfNC). All the isolates were able to grow in 2 to 10% NaCl but, failed to grow at 12% concentration and recorded growth from 10 to 42°C temperature. All the isolates grew in 6 to 8 pH range but failed to grow at 4, 5 and 10 pH. Bio-efficacy of the seven native strains of Pseudomonas spp. Was compared along with local commercial available biopesticides i.e. P. fluorescens (Aumgene, Pramukh and Pantnagar) and Hinosan. Three times spraying of P. aeruginosa Rambhas Strain at 0.6% (2×109 cfu/ml) at 15 days interval, starting at 21 days of transplanting was found significantly effective in the management of the leaf blast of finger millet (Magnaporthe grisea (Cke) Sacc.) by lowering disease intensity and increasing grain yield as well as fodder.
Pseudomonas spp., Isolates, Bioefficacy, Finger Millet Blast.
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