Development of Recombinant Cry3A Expressing Bacillus cereus Isolated from the Rhizosphere of Brinjal (Solanum melongena L.) in the Management of Ash Weevil (Myllocerus subfasciatus Guerin)


Affiliations

  • Indian Institute of Horticultural Research, Division of Biotechnology, Bangalore, Karnataka, 560 089, India

Abstract

A predominant Bacillus cereus (BR145) was engineered to express Cry3A protein by electroporation from B. thuringiensis tenebrionis (Btt.) The rhizospheric survival of these Bacillus spp., was compared where survival of the former was better in the rhizosphere of brinjal than the latter. The efficacy of Cry3A protein obtained from the lysed cells of rBR145 and Btt were evaluated for toxicity against grubs and adult M. subfasciatus in pot and detached leaf bioassays, respectively. The toxicity of Cry3A protein produced from both the bacilli was on par.

Keywords

Cry3A, Myllocerus subfasciatus, Bacillus cereus, Bacillus thuringiensis Ssp. Tenebrionis and Rhizosphere.

Subject Discipline

Agriculture Sciences

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