Identification of Antifungal Proteins from Fungal and Bacterial Antagonists against Colletotrichum falcatum Causing Sugarcane Red Rot
Effective fungal and bacterial antagonists having hyperparasitism and antibiosis mode of antagonistic activity against Colletotrichum falcatum were selected based on the suppression of pathogen growth and production of cell wall lytic enzyme chitinase. The effective strains were utilized to identify the sources of antifungal proteins suppressing enzymes and toxin, the pathogenicity determinants of Colletotrichum falcatum during their interection The effective biocontrol agents identified against C.falcatum inhibited the production of extracellular enzymes/proteins of the pathogen and also detoxified/inactivated the toxin/enzymes, when the antagonists were grown along with pathogen or its metabolites in liquid cultures as well as at the point of entry, where it failed to elicit characteristic symptoms on the host. Final outcome of the study resulted in identification of high molecular proteins specifically produced by the antagonists to inactivate the pathogenic metabolites in SDS-PAGE.
Sugarcane, Colletotrichum falcatum, Secondary Metabolites, Antifungal Proteins.
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