Effect of Trichoderma harzianum Isolated from Different Agroclimatic Zones of Karnataka on Growth and Biochemical Parameters of Nigella sativa L. a Medicinal Plant


  • University of Agricultural Sciences, Department of Biotechnology, Bangalore, Karnataka, 560 065, India


Trichoderma species are the fungi that are present in nearly all soils and other diverse habitats. Among Trichoderma genera Trichoderma harzianum is both plant growth promoter as well as antagonist against many pathogenic fungi. An effort was made to isolate ten isolates of T. harzianum from soil samples collected from different agroclimatic zones of Karnataka. A total of ten Trichoderma isolates representing each agroclimatic zone was isolated using potato dextrose agar medium and purified. The colony morphology and microscopic observation confirmed that all isolates belongs to the T. harzianum. A pot culture experiment was carried out to study the effect of the ten strains of T. harzianum on the medicinal plant Nigella sativa L. under glasshouse conditions. Growth parameters and biochemical studies were recorded in T. harzianum treated N. sativa along with untreated plants. The results indicated that there was a significant increase in plant height (24.07 cm), number of branches (19.33), fresh and dry weight of the plants (1.296 and 0.219 g/plant, respectively) inoculated with strain SDT isolated from zone 6 as compared to uninoculated control plants (13.03 cm, 8.00, 0.307 and 0.055 g/plant, respectively). However among the ten strains inoculated, T. harzianum isolates from the zone 6 (SDT) and zone 5 (EDT) recorded the highest biochemical parameters and least were recorded in uninoculated control plants.


Trichoderma harzianum, Agroclimatic Zones, Nigella sativa.

Subject Discipline

Agriculture Sciences

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