Influence of Storage Temperature, Population Density and Duration of Storage on the Survival of Three Species of Entomopathogenic Nematodes


  • Sugarcane Breeding Institute, Section of Entomology, Coimbatore, Tamilnadu, 641007, India


Among the five different temperatures tested for storage of infective juveniles (Ijs). survival of Steinernema feltiae Filipjev and S. glaseri Steiner was higher at 7.5°C, while the survival of Heterorhabditis indica (Poinar, Karunakar and David) was higher at 10°C. All the three species of nematodes showed higher percentage of survival when stored at 250 IJs per ml compared to 500 and 1000 Us per ml. A negative correlation was observed between duration of storage and per cent survival in S. feltiae (r = -0.9831**). S. glaseri (r = -0.9847**) and H. indica (r = -0.9437**). Based on the results of the study, the best combination of temperature, population density and duration of storage for the entomopathogenic nematodes was 7.5°C, 250 IJs perml and 120 days, respectively for S. feltiae, 7.5°C, 250 IJs per ml and 90 days for S. glaseri and 10°C, 250 IJs per ml and 90 days for H. indica.


Heterorhabditis indica, Population Density, Steinernema feltiae, S. glaseri, Storage.

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