TY - JOUR AU - Maheesha, M. AU - Balasubramani, V. AU - Murugan, M. AU - Raveendran, M. AU - Rajadurai, G. AU - Tamilnayagan, T. AU - Kokiladevi, E. AU - Sathiah, N. PY - 2022/06/05 Y2 - 2024/03/28 TI - Characterisation of native Bacillus thuringiensis isolates toxicity to fall armyworm, Spodoptera frugiperda (J.E. Smith) JF - Journal of Biological Control JA - JBC VL - 35 IS - 3 SE - Research Articles DO - 10.18311/jbc/2021/28812 UR - http://informaticsjournals.com/index.php/jbc/article/view/28812 SP - 171-180 AB - <p>Toxicity of nine indigenous <em>Bacillus thuringiensis</em> (<em>Bt</em>) isolates collected from Tamil Nadu, India were tested against fall armyworm,<em> Spodoptera frugiperda</em>. At 30 ?g/ml concentration, two <em>Bt</em> isolates <em>viz.</em>, T350 and T532 recorded 100 per cent mortality whereas isolates T527 and T532 registered 96 per cent mortality against neonate larvae of<em> S. frugiperda</em> in leaf dip bioassay. SDS PAGE analysis of spore crystal mixture revealed the presence of <em>Cry1</em> and <em>Cry2</em> proteins with visible bands at 130 kDa and 65 kDa. PCR screening results showed the presence of<em> cry1</em> (<em>cry1A, cry1Aa, cry1Ab</em>), <em>cry2</em> (<em>cry2Aa, cry2Ab</em>) in four isolates and vip3A genes in three isolates but <em>cry9</em> gene was not present in any of the isolates tested.</p> ER -