Amelioration of Trichosanthes lobata in Paracetamol-Induced Hepatic Damage in Rats: A Biochemical and Histopathological Evaluation


Affiliations

  • Department of Pharmacognosy, KLE University’s College of Pharmacy, Belagavi, Karnataka, 590010, India

Abstract

The aim of the present work is to investigate the possible therapeutic potential of Trichosanthes lobata in paracetamol induced hepatic damage in rats. The hepatotoxicity was induced in rats by administration of paracetamol at higher dose (2 gm/kg) reflected in term of increase lipid peroxidation, impairment in antioxidants [Superoxide Dismutase (SOD), Catalase (CAT) and Reduced Glutathione (GSH)] along with elevation of serum marker enzymes. The possible ameliorative effect of Trichosanthes lobata Leaf extracts mainly Aqueous (TLAQ) and Alcoholic (TLAL) were administered at various doses for 7days and found to restored the depleted antioxidants (SOD, GSH, CAT) attenuated lipid peroxidation (MDA formation) and prevented elevated marker enzymes significantly. Further, histopathological alterations were also improved with TLAQ and TLAL in dose dependent manner. Thus, the hepatoprotective activity of TLAQ and TLAL may be correlated with amelioration of oxidative stress by improving endogenous antioxidant defence ability of hepatocytes and histopathological alterations in rats. The ameliorative effect of Trichosanthes lobata in paracetamol-induced hepatic damage in rats was studied.

Keywords

Flavonoids, Hepatic Damage, Paracetamol, Trichosanthes lobata

Full Text:

References

Subramoniam A, Pushpangadan P. Development of phytomedicine for liver diseases. Indian J Pharmacol.1999; 31:166–75.

Wadekar RR, Supale RS, Tewari KM, Patil KS. Screening of roots of Baliospermum montanum for hepatoprotective activity against paracetamol induced liver damage in albino rats. International J Green Pharm. 2008; 2(4):220–3.https://doi.org/10.4103/0973-8258.44737

Bhandarkar M, Khan A. Antihepatotoxic effect of Nymphacea stellata Willd against Carbon tetrachloride hepatic damage in rats. J Ethnopharm. 2004; 91:61–4.https://doi.org/10.1016/j.jep.2003.11.020 PMid:15036469

Maheshwari C, Maryammal R, Venkatanarayan R.Hepatoprotective activity of Orthosiphon stamineus on liver damage caused by paracetamol in rats. Jor J of Biol Sci. 2008; 1(3):105–8.

Vaidyaratnam PS. Indian Medicinal Plants- A compendium of 500 species. Madras: Orient Longman Ltd; 1994.

Anonymous. The Wealth of India-Raw materials SP-W. 10th Publication and Information’s Directorate CSIR; New Delhi. 1976.

Harborne JB. Phytochemical methods: A guide to modern techniques of plant analysis. London: Chapman and Hall; 1998. PMid:28308423

Mukherjee PK. Quality Control of Herbal Drugs Business Horizon. New Delhi; 2002.

OECD423. Guidelines for the testing of chemicals. Revised draft guidelines acute oral toxicity acute toxic class methods; 2000.

Dash DK, Yelignar VC, Nayak SS, Ghosh T. Evaluation of hepatoprotective and antioxidant activity of Ichnocarpus frutescens (Linn) on paracetamol-induced hepatotoxicity in rats. Tro J Pharm Res. 2007; 6(3):755–65. https://doi.org/10.4314/tjpr.v6i3.14656

Reitme S, Frankel S. Colorimetric method for determination of glutamic oxaloacetate and glutamic pyruvic transaminase. American J Clin Patho. 1957; 28:56–63. https://doi.org/10.1093/ajcp/28.1.56

Amdor E. Serum Lactic dehydrogenase activity: An analytical assessment of current assay. Clin Chem. 1963; 9:391.

Malloy E, Evelyn K. The determination of bilirubin with the photoelectric colorimeter. J Biol Chem. 1978; 199:481–85.

Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem.1951; 193:265–75. PMid:14907713

Slater TF, Sawyer BC. The stimulatory effect on Carbon tetrachloride on peroxidative reaction on rat liver fractions invitro. Biochem J. 1971; 123:815–21. https://doi.org/10.1042/bj1230815

Ohkawa Η, Ohishi Ν and Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Ana Biochem. 1979; 95:351–8. https://doi.org/10.1016/00032697(79)90738-3

Mishra HP, Fridovich I. Role of superoxide anion in autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972; 247:3170–5.

Aebi H. Verlag Methods of enzymatic analysis. Verlag: Chemic Academic Press Inc; 1974.

Ellmen GL. Tissue sulfhydryl group. Arch Biochem and Biophy. 1959; 82:70–7. https://doi.org/10.1016/00039861(59)90090-6

Jollow DJ, Mitchell JR, Potter WZ, Davis DC.Acetaminophen induced hepatic necrosis II. Role of covalent binding in vivo. J Pharm and Exp Ther. 1973; 187:195–02. PMid:4746327

Haldar PK, Biswas M, Bhattacharya S, Karan TK, Ghosh AK. Hepatoprotective activity of Dregea volubilis fruit against paracetamol-induced liver damage in rats. Indian J Pharm Edu and Res. 2012; 46(1):17–21.

Sini S, Latha PG, Sasikumar JM, Rajashekaran S, Shyamal, S, Shine, VJ. Hepatoprotective studies on Hedyotis corymbosa. J Ethnopharmcol. 2006; 106:245–49. https:// doi.org/10.1016/j.jep.2006.01.002 PMid:16495024

Thabrew MI, Joice PDTM, Rajatissa WA. Comparative study of Pavetta indica and Osbeckia ictendra in the treatment of liver function. Plan Ind. 1987; 53:239–41.

Chang CH, Lin CC, Htoori M, Namba T. Effects of rosemary extracts and major constituents on lipid oxidation and soyabean lipoxygenase activity. J Am Oil Chem Soc. 1994; 69:999–02.

Naik SR, Thakare VN, Patil SR. Protective effect of curcumin on experimentally induced inflammation, hepatotoxicity and cardiotoxicity in rats: Evidence of its antioxidant activity. Exp Toxi Path. 2011; 63:419–31. https://doi.org/10.1016/j.etp.2010.03.001 PMid:20363603

Naure B, Zahia B, Ahmed M, et al. Antioxidant and Protective effect of Cynara cardunculus against paracetamol induced liver mitochondria oxidative stress.Int J Phyt. 2014: 601–7.

Shafy MM, Allah GM, Mohamadin AM, et al. Quercitin protection against acetaminophen induced hepatorenal toxicity by reducing reactive oxygen and nitrogen species.Pathophy. 2015; 22(1):49–55. https://doi.org/10.1016/j.pathophys.2014.12.002 PMid:25547049


Refbacks

  • There are currently no refbacks.