Phytochemical Characterization, In-Vitro Cytotoxic and Antibacterial Activity of Cotula cinerea (Delile) Vis Essential Oil


Affiliations

  • University of Hamma Lakhdar El-Oued, 39000, Algeria
  • Desert Research Center, Department of Ecophysiology, El-Mataria, 15753, Egypt

Abstract

Cotula cinerea is a traditional Algerian medicinal plant that has many biological properties, such as analgesic, antiseptic and antibacterial activities. Essential oil of Cotula cinerea was subjected to chromatographical and spectroscopical studies to determine the chemical composition. The analysis highlighted that the oil contains trans thujone (51.86%), santalina triéne (10.6%), α-pinéne (2.02%), sabinene (6.17%), cineole <1.8> (5.34%), δ-terpinene (1.57%), camphor (2.63%), β-terpineol (1.39 %) and terpin-4-ol (1.73%) as the major constituents. The essential oil was tested for antibacterial activity. The oil exhibited substantial antibacterial activity against both the tested Gram negative and Gram positive bacteria. In search of better anticancer agent the essential oil of Cotula cinerea was also subjected for in-vitro cytotoxic activity on two cancerous human cell lines; colon (HCT116) and hepatic (HePG2) cancer cell lines. The results indicates that the essential oil of Cotula cinerea has a significant cytotoxic activity on the tested colon cancer cell lines with a 66.9% inhibition and a minimal inhibition (33.9%) on liver cancer cell line.

Keywords

Essential Oil Composition, GC/MS, HCT116, HePG2.

Subject Discipline

organic chemistry

Full Text:

References

Abdenbi A, Abdelwahed D, Bouaaza M, Touati B. Screening Phytochimique et Activite Antibacterienne De L’Huile Essentielle De Cotula Cinerea (Gartoufa) Dans La Region De Bechar. Int J Res Eng Technol. 2014; 2(2): 49–54.

Markouk M, Redwane A, Lazrek HB, Jana M. Antibacterial activity of Cotula cinerea extracts. Fitoterapia. 1999; 70: 314–6. https://doi.org/10.1016/S0367-326X(99)00042-8

Redwane A, Markouk M, Lazrek HB, Amarouch H, Jana M. Laboratory evaluation of molluscicidal activity of extracts from Cotula cinerea (L) and Quercus lusitania var. infectoria galls (Oliv). Ann Pharm Fr. 1998; 56(6): 274–6. PMid:9872015

Dendougui H, Seghir S, Jay M, Benayache F, Benayache S. Flavonoids from Cotula cinerea Del. Int J Med Arom Plants. 2012; 2(4): 589–95.

Bensizerara D, Menasria T, Melouka M, Cheriet L, Chenchouni H. Antimicrobial activity of xerophytic plant (Cotula cinerea Delile) extracts against some pathogenic bacteria and fungi. Asian Pac J Trop Biomed. 2012: 1–5.

Markouk M, Redwane A, Lazrek HB, Jana M. Antibacterial activity of Cotula cinerea extracts. Fitoterapia. 1999; 70(3): 314–6. https://doi.org/10.1016/S0367-326X(99)00042-8

Review T. Environmental Science. 2014; Jul: 302–16.

Metwally MA, El-Dahmy S, Jakupovic J, Bohlmann F, Dawidar AM, Metwally SA. Glaucolide-like sesquiterpene lactones from Cotula cinerea. Phytochemistry. 1985; 25(1): 255–7. https://doi.org/10.1016/S0031-9422(00)94543-6

Atef C, Boualem M, Cherif M, Youcef H, Azzedine C. Chemical composition and antimicrobial activity of essential oils in xerophytic plant cotula cinerea Del (Asteraceae) during two stages of development: flowering and fruiting. J Appl Pharm Sci. 2015; 5(3): 029–34. https:// doi.org/10.7324/JAPS.2015.50306

Benhammou N, Ghambaza N, Benabdelkader S, AtikBekkara F, Kadifkova Panovska T. Phytochemicals and antioxidant properties of extracts from the root and stems of Anabasis articulata. Int Food Res J. 2013; 20(5): 2057–63.

Yá-ez X, Pinzón ML, Solano F, Sánchez LR. Chemical composition of the essential oil of Psidium caudatum McVaugh. Molecules. 2002; 7(9): 712–6. https://doi.org/10.3390/70900712 PMCid:PMC6146449

Vandendool H, Kratz PD. A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J Chromatogr. 1963; 11(3): 463–71. https://doi.org/10.1016/S00219673(01)80947-X

Al Abbasy DW, Pathare N, Al-Sabahi JN, Khan SA. Chemical composition and antibacterial activity of essential oil isolated from Omani basil (Ocimum basilicum Linn.). Asian Pacific J Trop Dis. 2015; 5(8): 645–9. https://doi.org/10.1016/S2222-1808(15)60905-7

Chang LP, Sheng LS, Yang MZ, An DK. Retention index of essential oil in temperature-programmed capillary column gas chromatography. Acta Pharm Sin. 1989; 24(11): 847–52.

May J, Chan CH, King A, Williams L, French GL. Timekill studies of tea tree oils on clinical isolates. The Journal of antimicrobial chemotherapy. 2000; 45: 639–43. https://doi.org/10.1093/jac/45.5.639 PMid:10797086

Sparkman OD. Identification of essential oil components by gas chromatography/quadrupole mass spectroscopy Robert P. Adams. J Am Soc Mass Spectrom. 2005; 16(11): 1902–3. https://doi.org/10.1016/j.jasms.2005.07.008

Ferreira A, Proenea C, Serralheiro MLM, Araejo MEM. The in vitro screening for acetylcholinesterase inhibition and antioxidant activity of medicinal plants from Portugal. J Ethnopharmacol. 2006; 108(1):31–7. https://doi.org/10.1016/j.jep.2006.04.010 PMid:16737790

Aligiannis N, Kalpoutzakis E, Mitaku S, Chinou IB. Composition and antimicrobial activity of the essential oils of two Origanum species. J Agric Food Chem. 2001; 49(9): 4168–70. https://doi.org/10.1021/jf001494m PMid: 11559104

Saadana D, Mahjoub MA, Boussaada O, Chriaa J, Charaif I, Daami M, et al. Chemical composition and antimicrobial activity of volatile compounds of Tamarix boveana (Tamaricaceae). Microbiol Res. 2008; 163(4): 445–55. https://doi.org/10.1016/j.micres.2006.07.009 PMid:17223327

Hammer KA, Carson CF, Riley T V. Antimicrobial activity of essential oils and other plant extracts. J Appl Microbiol. 1999; 86(6): 985–90. https://doi.org/10.1046/j.13652672.1999.00780.x PMid:10438227

El-Sharkawy ER, Matloub AA, Atta EM (2103). Cytotoxicity of new flavonoid compound isolated from Farsetia aegyptia. Int J Pharm Sci Invent. 2013; 2(1): 22–7.

Djellouli M, Benmehdi H, Mammeri S, Moussaoui A, Ziane L, Hamidi N. Chemical constituents in the essential oil of the endemic plant Cotula cinerea (Del.) from the southwest of Algeria. Asian Pac J Trop Biomed. 2015; 5(10): 870–3. https://doi.org/10.1016/j.apjtb.2015.06.007

Kasrati A, Alaoui Jamali C, Bekkouche K, Wohlmuth H, Leach D, Abbad A. Comparative evaluation of antioxidant and insecticidal properties of essential oils from five Moroccan aromatic herbs. J Food Sci Technol. 2015; 52(4): 2312–9. https://doi.org/10.1007/s13197-014-1284-z PMid:25829614 PMCid:PMC4375224

Bensizerara D, Menasria T, Melouka M, Cheriet L, Chenchouni H. Antimicrobial Activity of Xerophytic Plant (Cotula cinerea Delile) extracts against some pathogenic bacteria and fungi. Jordan Journal of Biological Sciences. 2013; 6(4): 266–71. https://doi.org/10.12816/0001624

Boussoula E, Ghanmi M, Satrani B, Alaoui MB, Rhafouri R, Farah A, Nadine A, Abdelaziz C. Chemical quality, antibacterial and antifungal activities of Cotula cinerea essential oil from South Morocco. ESAIJ. 2016; 12(5): 209–16.

El-Bouzidi L, Abbad A, Fattarsi K, Hassani L, Leach D, Markouk M, et al. Chemical composition and anticandidal properties of the essential oil isolated from aerial parts of Cotula cinerea: A rare and threatened medicinal plant in Morocco. Nat Prod Comm. 2011; 6(10): 1491–4. PMid:22164791

Elsharkawy ER, Hyaa G, Donia A. Comparison between Thuja orientalis growing in Egypt and Saudi Arabia. British Journal of Pharmaceutical Research. 2017; 15(5): 1–9. https://doi.org/10.9734/BJPR/2017/32387

Sieniawska E, Świątek Ł, Rajtar B, Koziol E, Polz‐Dacewicz M, Skalicka‐Wózniak K. Carrot seed essential oil-source of carotol and cytotoxicity study. https://doi.org/10.1016/j.indcrop.2016.08.001

Zarai Z, Ben Chobba I, Ben Mansour R, Békir A, Gharsallah N, Kadri A. Essential oil of the leaves of Ricinus communis L.: In vitro cytotoxicity and antimicrobial properties. Lipids in health and disease. 2012; 11: 102–108. https://doi.org/10.1186/1476-511X-11-102 1, 2.


Refbacks

  • There are currently no refbacks.