In Vitro Anti-cancer, Anti-hypertensive and Antihyperglycaemic effect of Hypoxis colchicifolia

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Authors

  • Suggessan Moodley
     Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, PO Box 1334, Durban 4000 ,ZA
  • Depika Dwarka
     Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, PO Box 1334, Durban 4000 ,ZA
  • Himansu Baijnath
     University of KwaZulu-Natal, School of Biological Sciences, Durban, KwaZulu-Natal ,ZA
  • John Jason Mellem
     Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, PO Box 1334, Durban 4000 ,ZA

DOI:

https://doi.org/10.18311/jnr/2021/25315

Keywords:

Anti-diabetic, Antihypertensive, Anticancer, Hypoxis colchicifolia

Abstract

Economic challenges associated with non-communicable diseases and the sociocultural outlook of many patients especially in Africa has increased the dependence on traditional herbal medicines for these diseases. Hypoxis colchicifolia is a traditional medicinal plant used in Southern Africa against an array of ailments. This study evaluated the in vitro antidiabetic (α-amyalse and α-glucosidase), antihypertensive (angiotensin-converting enzyme) and anticancer potential of H. colchicifolia corm as well as leaf (acetone, methanol and aqueous) extracts. Results showed that extracts have a moderate anti-diabetic and anti-hypertensive potential, with great anti-cancer potential. The acetone extract of both fresh and dried corms produced significant α-amylase and α-glucosidase inhibition with ACE inhibited predominantly by the dried corms methanolic extract (IC50 368.2 μg/mL). Methanolic extract of dried leaves showed the least cytotoxicity against the noncancerous cell line HEK-293 while exhibiting the highest inhibition of MCF-7 cells (IC50 3.24 μg/mL). All extracts exhibited a greater inhibitory potential in A549 cells than the positive control camptothecin (IC50 304.2μg/mL). This study reveals that H. colchicifolia has therapeutic potential as an anti-diabetic and anticancer agent; however, further in vivo studies need to be conducted.

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Published

2021-07-06

How to Cite

Moodley, S., Dwarka, D., Baijnath, H., & Jason Mellem, J. (2021). In Vitro Anti-cancer, Anti-hypertensive and Antihyperglycaemic effect of <i>Hypoxis colchicifolia</i>. Journal of Natural Remedies, 21(3), 209–217. https://doi.org/10.18311/jnr/2021/25315

Issue

Volume 21, Issue 3, July 2021

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC
Received 2020-05-11
Accepted 2020-12-16
Published 2021-07-06

 

References

Forouzanfar MH, Afshin A, Alexander LT, Anderson HR, Bhutta ZA, Biryukov S, et al., 2016. Global, regional, and national comparative risk assessment of 79 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990-2015: A systematic analysis for the Global Burden of Disease Study. The Lancet. 2015; 388:1659-724. https://doi.org/10.1016/S0140-6736(16)31679-8

Abegunde DO, Mathers CD, Adam T, Ortegon M, Strong K. The burden and costs of chronic diseases in low-income and middle-income countries. The Lancet. 2007; 370:1929-38. https://doi.org/10.1016/S0140-6736(07)61696-1

Kiringe JW. A survey of traditional health remedies used by the Maasai of Southern Kaijiado District, Kenya. Ethnobotany Research and Applications. 2006; 4:61-74. https://doi.org/10.17348/era.4.0.61-74

Shapiro K, Gong WC. Natural products used for diabetes. J Am Pharm Assoc. 2002; 42:217-26. https://doi.org/10.1331/108658002763508515. PMid:119266 65

Kamtekar S, Keer V, Patil V. Estimation of phenolic content, flavonoid content, antioxidant and alpha amylase inhibitory activity of marketed polyherbal formulation. J Appl Pharm Sci. 2014; 4:61.

Odhav B, Kandasamy T, Khumalo N, Baijnath H. Screening of African traditional vegetables for their alpha-amylase inhibitory effect. J Med Plant Res. 2010; 4:1502-7.

Braga FC, Serra CP, Júnior NSV, Oliveira AB, Cí´rtes SF, Lombardi JA. Angiotensin-converting enzyme inhibition by Brazilian plants. Fitoterapia. 2007; 78:353-8. https://doi.org/10.1016/j.fitote.2007.02.007. PMid:17513067

Ranilla LG, Apostolidis E, Genovese MI, Lajolo FM, Shetty K. Evaluation of indigenous grains from the Peruvian Andean region for antidiabetes and antihypertension potential using in vitro methods. J. Med. Food. 2009; 12:704-13. https://doi.org/10.1089/jmf.2008.0122. PMid:19735168

Cragg GM, Newman DJ. Plants as a source of anticancer agents. J Ethnopharmacol. 2005; 100:72-9. https://doi.org/10.1016/j.jep.2005.05.011. PMid:1600 9521

Patel SG. A review on medicinal plants for cancer therapy. International Journal of MediPharm. 2016; 2:105-12.

Assaf AM, Haddadin RN, Aldouri NA, Alabbassi R, Mashallah S, Mohammad M, et al., Anti-cancer, anti-inflammatory and anti-microbial activities of plant extracts used against hematological tumors in traditional medicine of Jordan. J Ethnopharmacol. 2013; 145:7280-36. https://doi.org/10.1016/j.jep.2012.11.039. PMid:23246454

Bisi-Johnson MA, Obi CL, Samuel BB, Eloff JN, Okoh AI. Antibacterial activity of crude extracts of some South African medicinal plants against multidrug resistant etiological agents of diarrhoea. BMC Complement Altern Med. 2017; 17:321. https://doi. org/10.1186/s12906-017-1802-4. PMid:28629407. PM Cid:PMC5474864

Moodley S, Dwarka D, Baijnath H,Mellem J. Antioxidant and phenolic constituents of Hypoxis colchicifolia Fascicle VI. Food Technol.2020. In Press.

Ranilla LG, Kwon Y-I, Apostolidis E, Shetty K. Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin America. Bioresour Technol. 2010; 101:4676-89. https://doi.org/10.1016/j.biortech.2010.01.093. PMid:20185303

Li G-H, Liu H, Shi Y-H, Le G-W. Direct spectrophotometric measurement of angiotensin I-converting enzyme inhibitory activity for screening bioactive peptides. J Pharm Biomed Anal J Pharmaceut Biomed. 2005; 37:219-24. https://doi.org/10.1016/j.jpba.2004.11.004. PMid:15708660

Chen J, Wang Y, Ye R, Wu Y, Xia W. Comparison of analytical methods to assay inhibitors of angiotensin I-converting enzyme. Food Chem. 2012; 141:3329- 34. https://doi.org/10.1016/j.foodchem.2013.06.048. PMid:23993489

Dwarka D, Thaver V, Naidu M, Koorbanally NA, Baijnath H. In vitro chemo-preventative activity of Strelitzia nicolai aril extract containing bilirubin. Afr JTradit Complement Altern Med.2017; 14:147- 56. https://doi.org/10.21010/ajtcam.v14i3.16. PMid: 28480426. PMCid:PMC5412220

Akinrinde A, Koekemoer T, van de Venter M, Bradley G. In vitro investigation of potential anti-diabetic activity of the corm extract of Hypoxis argentea Harv. ex Baker. Acta Pharm.2018; 68:389-407. https://doi.org/10.2478/acph-2018-0023. PMid:31259706

Alimi AA, Ashafa AOT. An in vitro evaluation of the antioxidant and antidiabetic potential of Sutherlandia montana E. Phillips & R.A. Dyer leaf extracts. Asian Pac J Trop Biomed.2017; 7:765-72. https://doi.org/10.1016/j.apjtb.2017.08.004

Oguntibeju OO, Meyer S, Aboua YG, Goboza M. Hypoxis hemerocallidea significantly reduced hyperglycaemia and hyperglycaemic-induced oxidative stress in the liver and kidney tissues of streptozotocin-induced diabetic male wistar rats. Evid-Based Compl Alt. 2016. https://doi.org/10.1155/2016/8934362. PMid:274032 00. PMCid:PMC4925985

Nair SS, Kavrekar V, Mishra A. In vitro studies on alpha amylase and alpha glucosidase inhibitory activities of selected plant extracts. Eur J Exp Biol. 2013; 3:128-32.

Sama K, Murugesan K, Sivaraj R. In vitro alpha amylase and alpha glucosidase inhibition activity of crude ethanol extract of Cissus arnottiana. Asian J Plant Sci.2012; 2:550-3.

Duncan AC, Jäger AK, van Staden J. Screening of Zulu medicinal plants for angiotensin converting enzyme (ACE) inhibitors. J Ethnopharmacol. 1999; 68:63-70. https://doi.org/10.1016/S0378-8741(99)00097-5

Arhin I, Dwarka D, Bissessur A, Naicker D, Mackraj I. Biochemical, phytochemical profile and Angiotensin-1 converting enzyme inhibitory activity of the hydro-methanolic extracts of Tulbaghia acutiloba Harv. J Nat Remedies.2019; 19:221-35. https://doi.org/10.18311/jnr/2019/23485

Madikizela B, Mcgaw LJ. Scientific rationale for traditional use of plants to treat tuberculosis in the eastern region of the OR Tambo district, South Africa. J Ethnopharmacol. 2018; 224:250-60. https://doi.org/10.1016/j.jep.2018.06.002. PMid:29870786

Chavan SS, Damale MG, Shamkuwar PB, Pawar DP. Traditional medicinal plants for anticancer activity. Int J Curr Pharm Res.2013;5:50-4.

Steenkamp V,Gouws M. Cytotoxicity of six South African medicinal plant extracts used in the treatment of cancer. S Afr J Bot. 2006; 72:630-3. https://doi.org/10.1016/j.sajb.2006.02.004

Boukes GJ,van de Venter M. Cytotoxicity and mechanism (s) of action of Hypoxis spp. (African potato) against HeLa, HT-29 and MCF-7 cancer cell lines. J Med Plant Res. 2011; 5:2766-74.

Madikizela B,Mcgaw L. In vitro cytotoxicity, antioxidant and anti-inflammatory activities of Pittosporum viridiflorum Sims and Hypoxis colchicifolia Baker used traditionally against cancer in Eastern Cape, South Africa. S Afr J Bot. 2019;126:250- 5. https://doi.org/10.1016/j.sajb.2019.06.009