An HPLC Method for Simultaneous Estimation of Psoralen, Bakuchicin and Bakuchiol in Psoralea corylifolia


  • Natural Remedies Pvt. Ltd., Department of Pharmacology, Bioassay Research Laboratory, Research and Development Centre, Bangalore, 561229, India
  • Natural Remedies Pvt. Ltd., Research and Development Centre, Bangalore, 561229, India
  • St. John's Medical College, Department of Pharmacology, Bangalore, 560034, India


Objective: To develop an HPLC method for estimation of psoralen, bakuchicin and bakuchiol in Psoralea corylifolia. Materials and methods: An gradient, reverse phase (RP) HPLC procedure using a mixture of Phosphate buffer and acetonitrile as mobile phase, C18 column as stationary phase and UV detector. Results: The developed method shows high resolution, accuracy and reproducibility. Conclusions: The method developed is accurate, precise and specific.


Psoralea corylifolia, Psoralen, Bakuchicin, Bakuchiol, HPLC.

Subject Discipline

Pharmacy and Pharmacology

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