Flavonoid Content of Eupatorium glandulosum and Coolebroke oppositifolia


  • JSS College of Pharmacy, Department of Pharmaceutical Chemistry, Ootacamund, Tamil Nadu, 643001, India
  • Leiden/Amsterdam Center for Drug Research, Division of Pharmacognosy, Gorleaus Laboratories, Leiden, Netherlands
  • JSS College of Pharmacy, Department of Pharmacology, Ooty, Tamil Nadu, 643001, India


Objective: Estimation of quercetin content of Eupatorium glandulosum and Coolebroke oppositifolia by HPLC. Materials and methods: The presence of quercetin (3, 3I, 4I, 5, 7 - pentahydroxyflavone) present in the leaves of Eupatorium glandulosum (Family- Asteraceae) and Coolebroke oppositifolia (Family : Labiatae) was quantitatively estimated by high performance liquid chromatography using reversed phase (RP-HPLC) with stepwise gradient elution on a RP C18 column. A two step elution with: acetonitrile- water- phosphoric acid (85%): from 16-83-1 to 32-67-1(v/v) was made. Results: The leaf extracts of E. glandulosum and C. oppositifolia contained 4.96 and 2.10 % and the respective powdered leaf contained 0.19 % (w/w) and 0.09 % (w/w) of quercetin . Conclusion: The method developed is useful to obtain quantitative values for quercetin present in these plant species, which may be useful for standardization of the same.


Eupatorium glandulosum, Coolebroke oppositifolia, Flavonoid, Quercetin, RP-HPLC.

Subject Discipline

Pharmacy and Pharmacology

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