Flavonoid Content of Eupatorium glandulosum and Coolebroke oppositifolia

Abstract

Objective: Estimation of quercetin content of Eupatorium glandulosum and Coolebroke oppositifolia by HPLC. Materials and methods: The presence of quercetin (3, 3^I, 4^I, 5, 7 - pentahydroxyflavone) present in the leaves of Eupatorium glandulosum (Family- Asteraceae) and Coolebroke oppositifolia (Family : Labiatae) was quantitatively estimated by high performance liquid chromatography using reversed phase (RP-HPLC) with stepwise gradient elution on a RP C18 column. A two step elution with: acetonitrile- water- phosphoric acid (85%): from 16-83-1 to 32-67-1(v/v) was made. Results: The leaf extracts of E. glandulosum and C. oppositifolia contained 4.96 and 2.10 % and the respective powdered leaf contained 0.19 % (w/w) and 0.09 % (w/w) of quercetin . Conclusion: The method developed is useful to obtain quantitative values for quercetin present in these plant species, which may be useful for standardization of the same.