Cytomorphologic Analysis of Body Fluids

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Authors

  • Snehal Dnyandeorao Bhade
     PG Resident, Department of Pathology, Dr. Vasantrao Pawar Medical College and Research Centre, Nashik − 422003, Maharashtra ,IN
  • Archana M. Ukey
     Associate Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College and Research Centre, Nashik − 422003, Maharashtra ,IN
  • Sheela Chikhalikar
     Assistant Professor, Department of Pathology, Dr. Vasantrao Pawar Medical College and Research Centre, Nashik - 422003, Maharashtra ,IN

DOI:

https://doi.org/10.18311/mvpjms/2018/v5i2/18266

Keywords:

Adenosine De-Aminase (ADA), Body Fluids, Cytomorphology, Neoplasia
Pathology

Abstract

Background: Many etiologies result in development of effusions in the serous cavities. Of these pleural, peritoneal, pericardial fluids and cerebrospinal fluid are the most common. Cytologic study is done to diagnose disease ranging from benign (infective and inflammation) to malignant effusion1. Setting and Study Design: Pleural, peritoneal, and cerebrospinal fluid were included and others were excluded. In this study the significance of fluid cytology in the diagnosis of various neoplastic and non neoplastic lesions in the cytology section was carried out. Materials and Methods: All samples received in the Department of Pathology - pleural fluid, ascitic fluid and CSF were evaluated. Gross Examination of fluids was done for total Volume, Colour and Clarity. Biochemical test correlation of fluid Adenosine De-Aminase (ADA) levels was done wherever necessary. Diagnosis was done by consultants who were posted in cytopathology section. Results: In this prospective study from August 2015-2017, total 146 cases were studied of which 65 cases were of ascitic fluid, 41 of pleural fluid and 40 cases of CSF. Of these 146 cases, benign cases were 134 (91.78%), malignant effusion 9(6.16%) and suspicious of malignancy were 3 (2.05%). Among 146 study cases, 136 (93%) cases were having similar cytomorphological pattern (singly scattered), other 10 cases showed various patterns such as 3-dimensional clusters, sheets, etc. Conclusion: Body fluid examination with correlation of various parameters like thorough clinical history and examination, different serum marker levels, primary malignancy if present and previous cytological diagnosis are very useful for the final diagnosis.

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Published

2019-04-17

Issue

Volume 5, Issue 2, July - December 2018

Section

Original Research Article

 

References

Rasik Nathabhai Hathila, Reena Balvantbhai Dudhat, Peeyush Kumar Saini, Sonalben Laxmanbhai Italiya, KumarBhargav Ramesh chandra Kaptan, Mitesh kumar Bhailalbhai Shah. Diagnostic importance of serous fluid examination for detection of various pathological conditions - A study of 355 cases, International Journal of Medical Science and Public Health. 2013; 2(4): 975−79. DOI: 10.5455/ijmsph.2013.090720134. https://www.ijmsph.com/?mno=40616.

Naylor B. Pleural, Peritoneal, and Pericardial Effusions. Comprehensive Cytopathology; 2008. p. 515−77. DOI: 10.1016/B978-141604208-2.10019-3.

Edmund S. Cibas. Pleural, Pericardial and Peritoneal Fluids, Cytology. 2009; 129−537.

Koss LG, Melamed MR (Eds). Koss' Diagnostic cytology and its histopathologic bases.5th Ed., Philadelphia: JB Lippincott Company; 2006.

Ehya H. Effusion cytology, Clinics in Laboratory Medicine. 1991; 11(2):443−67. https://doi.org/10.1016/S02722712(18)30563-8.

Junaid TA. Cytologic diagnosis of ascitic fluid in Ibadan, Nigeria, Journal of the National Medical Association. 1980; 72(7):669−72. PMid: 7392084, PMCid: PMC2552510.

Kashyap RS, Kainthla RP, Mudaliar AV, Purohit HJ, Taori GM, Daginawala HF. Cerebrospinal fluid adenosine deaminase activity: A complimentary tool in the early diagnosis of tuberculous meningitis, Cerebrospinal Fluid Research. 2006; 3:5. https://doi.org/10.1186/1743-8454-3-5. PMid: 16571142, PMCid: PMC1448186.

Gupta BK, Bharat Vinay, Bandyopadhyay Debapriya. Sensitivity, specificity, negative and positive predictive values of Adenosine deaminase in patients of Tubercular and Non-Tubercular Serosal Effusion in India, Journal of Clinical Medicine Research. 2010 Jun; 2(3):121–26. PMid: 21629524, PMCid: PMC3104643.

Satya Vati Rana, Raj Kumar Singhal, Kartar Singh, Lata Kumar. Adenosine deaminase levels in Cerebrospinal fluid as a diagnostic test for Tuberculous Meningitis in children, Indian Journal of Clinical Biochemistry. 2004; 19(2):5–9. https://doi.org/10.1007/BF02894249. PMid: 23105448, PMCid: PMC3454202.

Rajesh Baheti, Purnima Laddha, RS Gehlot. CSF Adenosine Deaminase (ADA) activity in various types of meningitis, Journal Indian Academy of Clinical Medicine. 2001 Oct-Dec; 2(4):285–87.

Ribera E, Martinez-Vazquez JM, Ocana I, Segura RM, Pascual C. Activity of adenosine deaminase in cerebrospinal fluid for the diagnosis and follow-up of tuberculous meningitis in adults, The Journal of Infectious Diseases. 1987; 155(4):603–07. https://doi.org/10.1093/infdis/155.4.603. PMid: 3102627.

Valdés L, San José ME, Pose A, et al. Diagnosing tuberculous pleural effusion using clinical data and pleural fluid analysis: a study of patients less than 40 years-old in an area with a high incidence of tuberculosis, Respiratory Medicine. 2010; 104(8):1211−17. https://doi.org/10.1016/j.rmed.2010.02.025. PMid: 20347287.

Jiménez Castro D, Dí­az Nuevo G, Pérez-Rodrí­guez E, et al. Diagnostic value of adenosine deaminase in nontuberculous lymphocytic pleural effusions, European Respiratory Journal. 2003; 21:220−24. https://doi.org/10.1183/0903193 6.03.00051603. PMid: 12608433.

Sakuraba M, Masuda K, Hebisawa A, et al. Pleural effusion Adenosine De-Aminase (ADA) level and occult tuberculous pleurisy, Annals of Thoracic and Cardiovascular Surgery. 2009; 15:294−96. PMid: 19901882.

Pradhan SB, Pradhan B, Dali S. Cytology of body fluids from different sites: An approach for early diagnosis of malignancy, Journal of Nepal Medical Association. 2006; 45:353−56. PMid: 17676071.