2, 5-Hexanedione-Induced Oxidative Damage and DNA Fragmentation:Ameliorative Role of Rutin Ex Vivo

Abstract

The aim of this research was to evaluate the ameliorative effects of rutin on 2, 5-hexanedione-induced oxidative damage and DNA fragmentation in the viscera of Wistar rat ex vivo. Blood and tissues homogenates from normal Wistar rats weighing 150-200 g were used for the present investigation. The treatment with 2,5-hexanedione and/or rutin was for four (4), eight (8) and sixteen (16) hours incubation period at room temperature, and used non-specifically at the final concentrations of 0.0, 10.0 μM, 100.0 μM, 1.0 mM and 10.0 mM respectively. The experiments were done in four sequential stages involving the determination of lipid peroxidation and DNA fragmentation by standard procedures. 2,5-hexanedione dose/time-dependently caused a significant (P < 0.05) oxidative damage in the blood and tissues via lipid peroxidation and DNA fragmentation relative to control. Rutin administration was able to significantly (P < 0.05) suppress the 2,5-hexanedione-induced oxidative and DNA damage. However, the effective toxicity of 2,5-hexanedione and ameliorative effects of rutin were most versus least pronounced in liver (at 10 μM) and pancreas (at 1 mM) homogenates at 8hrs incubation time, respectively. 2,5-hexanedione in the viscera of Wistar rat induces oxidative and DNA damage characterised by higher level of malondialdehyde and DNA fragmentation. However, rutin administration was able to ameliorate these effects ex vivo