Effect of repeated dermal application of α-cypermethrin on lipid peroxidation and antioxidant system in rats


Affiliations

  • Sher-e-Kashmir University of Agricultural Sciences and Technology – Jammu, Division of Pharmacology and Toxicology,, Jammu, 181102, India
  • Sher-e-Kashmir University of Agricultural Sciences and Technology – Jammu, Division of Pharmacology and Toxicology, Jammu, 181102
  • Sher-e-Kashmir University of Agricultural Sciences and Technology – Jammu, Division of Pharmacology and Toxicology, Jammu, 181102, India

Abstract

α-Cypermethrin, a pyrethroid insecticide has a wide use for crop protection and ectoparasitic control in domestic animals. Excessive dermal exposure due to environmental contamination or inadvertent use may result in hazardous effects in man and animals. In present study the various biomarkers for the oxidative stress have been studied in Wister rats. SOD and catalase activities in erythrocytes were significantly decreased after 30 day of dermal exposure although these were initially over expressed. There was significant decrease (p<0.05) in blood GSH level and GPx activities in all the three groups. Enzymatic activity of GST was not significantly affected on any day of the exposure. Significant increase in lipid peroxidation of erythrocyte membrane from 10 days onwards exposure was also observed. The evaluated enzymatic and non-enzymatic components of the cell suggest that dermal exposure of ?-cypermethrin produces oxidative stress in rats.

Keywords

α-cypermethrin, antioxidant system, dermal application, lipid peroxidation

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References

Aebi, H (1983): Catalase, In: Methods Enzymol (Eds. Bergmeyer. H.U.) Vol.3, pp, 276-286); Academic Press, New York.

Andreyev, AY, Kushnareva, YE and Starkov, AA (2005): Mitochondrial metabolism of reactive oxygen species. Biochem. (Mosc), 70: 200-214.

Belma, G, Aylin, G and Filiz, H (2001): Cypermethrin-induced oxidative stress in rat brain and liver is prevented by Vitamin E or allopurinol. Toxicol. Letters, 118(3): 139-146.

Beutler, E, (1975): Red cell metabolism. In: A manual of biochemical methods, New York: Grune Strottan, 67-69.

Braughler, JM and Hall, ED (1989): Central nervous system trauma and stroke. I. Biochemical considerations for free radical formation and lipid peroxidation. Free Rad. Biol. Med., 6: 289-301.

Butterfield, DA, Darke, J, Pocernich, C and Castegna, A (2001): Evidence of oxidative damage in Alzheimer’s disease brain: Central role of Amyloid beta-peptide. Trends Molecular Med., 7: 548-554.

Chance, B, Sies, H and Boveris, A (1979): Hydroperoxide metabolism in mammalian organs. Physiolog. Rev., 59: 527-605.

Chow, CK and Tappel, AL (1972): An enzymatic protective mechanism against lipid peroxidation damage to lungs of ozone-exposed rats. Lipids, pp. 7518-7524.

Cross, CE (1987): The spectrum of diseases, in: Cross CE, moderator Oxygen radicals and human disease. Ann. Intern. Med., 107:526-45.

Daniel, KL and Moser, VC (1993): Utility of a neurobehavioral screening battery for differentiating the effects of two pyrethroids, permethrin and cypermethrin. Neurotoxicol. Teratol., 15: 71-83.

Edes, I, Piros, G, Toth, GK, Csanady, M and Kovacs, K (1986): Effects of long term alcohol ingestion on glutathione metabolizing enzymes and taurine contents in the myocardium of rats. Cardiovascular Res., 20: 918-21.

Elliot, M and Janes, NF (1978): Synthetic pyrethroid a new class of insecticides. Chem. Soc. Rev., 7: 473-505.

Fridovich, I (1995): Superoxide radical and superoxide dismutases. Annu. Rev. Biochem., 64: 97-112.

Gowenlock. AH (1996): Varley’s practical clinical biochemistry, 6th edition, New Delhi: CBS publishers 664-665.

Habig, WH, Pabst, MJ and Jakoby, WB (1974): Glutathione S-transferase. The first enzymatic step in mercapturic acid formation. J. Biol. Chem., 249: 7130-7139.

Hafeman DG, Sunde RA and Hoekstra WG (1974) Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J. Nutr., 104: 580-587.

Halliwell, B and Gutteridge, JMC (1985): Oxygen radicals and the nervous system. Trends Neuroscience, 8: 22-26.

Halliwell, B and Gutteridge, JMC (1989): Free radicals in biology and medicine, 2nd edition, Clarendon press, Oxford. pp 188-276.

Hayes, JD and Pulford, DJ (1995): The glutathione S-transferase super gene family. Regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit. Rev. Biochem. Mol. Biol., 30:445-600.

Kushnareva, Y, Murphy, AN and Andreyev, A (2002): Complex mediated reactive oxygen species generation: modulation by cytochrome c and NAD(P)+ oxidationreduction state. Biochem. J., 368: 545-553.

Luty, S, Latuszynsika, J, Halliop, J, Tochman, A, Przylepa, OE and Korczak, E (1998) Toxicity of dermally applied alpha-cypermethrin in rats. Ann. Agric. Environ. Med. 5: 109-115.

Marklund, S and Marklund, M (1974): Involvement of superoxide anion radical in autoxidation of pyrogallol and a convenient assay of superoxide dismutase. European J. Biochem., 47: 469-474.

Maxwell, SR (1995). Prospects for the use of antioxidant therapies. 449 Drugs, 49: 345-361.

Meister, A and Anderson, ME (1983): Glutathione. Ann. Rev. Biochem., 545(52): 711-760.

Mohammad, A (2002): Oxidative stress and experimental carcinogenesis. Indian J. Exp. Biol., 40: 656-667.

Ohkawa, H, Ohishi, N and Yagi, K (1979): Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 95: 351-358.

Punareewattana, K, Smith, BJ, Blaylock, BL, Longstreth, J, Snodgrass, HL, Gogal, RM, Prater, RM and Holladay, SD (2001): Topical permethrin exposure inhibits antibody production and macrophage function in C57Bl/ 6N mice. Food and Chem. Toxicol., 39: 133-139.

Radi, R, Turrens, JF, Chang, LY, Bush, KM, Crapo, JD and Freeman, BA (1991): Detection of catalase in rat heart mitochondria. J. Biol. Chem., 266: 22028–22034.

Sen, T, Sen, N, Tripathy, G, Chatterjee, U and Chakraborty, S (2006): Lipid peroxidation associated cardiolipin loss and membrane depolarization in rat brain mitochondria. Neurochem. Int., 49: 20-27.

Singh, SN, Vats, P, Kumria, MM, Ranganthan, S, Shyam, R, Arora, MP, Jain, CL and Sridharan, K (2001): Effect of high altitude (7620m) exposure on glutathione and related metabolism in rats. European J. Physiol., 84: 133-137.

Trojanowski, JQ (2003): Rotenone neurotoxicity: a new window on environmental causes of Parkinson’s disease and related brain amyloidoses. Exp. Neurol., 179: 6-8.

Tsan, MF, Clark, RN, Goyert, SM and White, JE (2001): Induction of TNF-? and MnSOD by endotoxin: role of membrane CD14 and Toll-like receptor-4. American J.Cell Physiol., 280: C1422-1430.


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