Single dose neurotoxicity screening studies of insecticide combination (Cypermethrin and Profenofos) in Wistar rats

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Authors

  • E. Rajesh
     Division of Toxicology, Department of Zoology, M.S. University of Baroda, Vadodara, Gujarat ,IN
  • B. Pilo
     Division of Toxicology, Department of Zoology, M.S. University of Baroda, Vadodara, Gujarat ,IN

Keywords:

Cypermethrin, Profenofos, FOB, Neurotoxicity, Neurobehaviour, Motor activity

Abstract

The present investigation has been taken up to determine the toxic effects of combination insecticide (Profenofos 40% + Cypermethrin 4% EC) on nervous system of rats. A single oral dose of test substance was administered to five rats per sex at a concentration of 25, 75 and 225mg/kg body weight to experimental animals. The animals were observed under carefully standardized conditions with sufficient frequency to ensure the detection and quantification of behavioral and/ or neurologic abnormalities. In addition, serum cholinesterase was estimated after 24 hours of dosing. Motor activity of individual animal was measured using an automated device. Perfused (in-situ) nervous tissues were examined microscopically. The test article at the dose level of 75 and 225 mg/kg induced treatment related and dose dependent neurobehavioural alterations in rats of either sex. However, observed alterations were reversible, further no structural/ histopathological alterations were apparent. Single dose of test article at 25 mg/kg did not reveal any toxic signs or neurobehavioral alterations hence, it is considered to be the No Observed Adverse Effect Level (NOAEL).

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Published

2018-04-06

How to Cite

Rajesh, E., & Pilo, B. (2018). Single dose neurotoxicity screening studies of insecticide combination (Cypermethrin and Profenofos) in Wistar rats. Toxicology International, 16(2), 97–102. Retrieved from http://informaticsjournals.com/index.php/toxi/article/view/20863

Issue

Volume 16, Issue 2, May-August 2009

Section

Original Research
Received 2018-04-06
Accepted 2018-04-06
Published 2018-04-06

 

References

Ballantyne, B., Marrs, T.C. and Turner, P. (1995). "Fundamentals of Toxicology”. In: General and Applied Toxicology. (Eds.) Ballantyne, B., Marrs, T.C. and Turner, P., Macmillan Press Ltd., the Bath Press, Avon. pp. 3-38.

Bloomquist, J.R. (1993). Neuroreceptor mechanisms in pyrethroid mode of action and resistance. In: Reviews in pesticide toxicology. Roe RM, Kuhr RJ, eds Raleigh, N.C. Toxicology Communications Inc., pp.184-230.

Brar, R.S., Sandhu, H.S. and Singh, A. (2000). "Histopathological Techniques”. In: Veterinary Clinical Diagnosis by Laboratory Methods, 1st Edition, Kalyani Publisher, 32: 186-196.

Casida, J.E. (1963). Pyrethrum flowers and pyrethroid insecticides. Environ. Hlth. Perspect., 34:189-202.

Carey, N. Pope (1999). "Organophosphorous Pesticides: Do they all have the same mechanism of toxicity?”. Journal of Toxicology and Environmental Health Part B: Critical Reviews. Publisher: Taylor & Francis. 2:161-181.

DuBois, K.P. (1971). "The Toxicity of Organophosphorous compounds to mammals”. Bull. World Health Organ., 44: 233-240.

DeBleecker, J., Van Den Neucker, K. and Colardyn, F.(1993). Intermediate Syndrome in Organophosphorus

Poisoning: A Prospective Study. Crit. Care Med., 21: 1706-1711.

Edwards, P.M. and Parker, V.H. (1997). A Simple, Sensitive and Objective Methods for Early Assessment of Acrylamide Neuropathy in Rats. Toxicol. and Appl. Pharmacol., 40: 589-591.

Moser, V.C. (1996). "Screening Approaches to Neurotoxicity: A functional observational battery”. J. American Toxicol., 8: 85-93.

Moser, V.C., McCormick, J.P., Creason, J.P. and MacPhail, R.C. (1988). "Comparison of Chlordimeform and Carbaryl Using a Functional Observational Battery”. Environ. and Appl. Toxicol., 11: 189-206.

Mattsson, J.L., Wilmer, J.W., Shankar, M.R., Berdasco, N.M., Crissman, J.W., Maurissen, J.P. and Bond, D.M. (1986). "Single dose and 13 week Repeated Dose Neurotoxicity Screening Studies of Chlorpyrifos Insecticide”. Fd. Chem. Toxicol., 34 : 393-405.

McDaniel, K.L. and Moser, V.C. (2004). "Differential profiles of cholinesterase inhibition and neurobehavioral effects in rats exposed to fenamiphos or profenofos”. Neurotoxicol. Teratol., 26: 407-415.

Motulsky, H.J. (1999). "Analyzing Data with GraphPad Prism”. GraphPad Software Inc., San Diego CA.

Nolan, M.P. and Roberson, E.L. (1979). Veterinary Pharmacology and Therapeutics. Oxford and IBH Publication, Co., New Delhi, pp.1107-1111.

O'Brien, R.D. (1967). "Toxic Phosphorus Esters: Chemistry, Insecticides, Action and Metabolism”. Academic Press, Inc., New York.

Righi, D.A. and Palmermo-Neto, J. (2003). "Behavioural effects of type II pyrethroid cyhalothrin in rats”. Toxicol. Appl. Pharmacol., 191: 76-167.

Stevens, J.T. and Breckenridge, C.B. (2001). ”Crop Protection Chemicals”. In: Principles and Methods of Toxicology. 4th Ed. Edited by: A. Wallace Hayes. Taylor & Francis, Philadelphia, pp. 565-648.

Taylor, P. (1985). "Anticholinesterase agents”. In: The Pharmacological Basis of Therapeutics. Gilman AG and Goodman LS (eds), New York: Macmillan Publishing Co.Inc., pp. 28-110.

Weiss, B. (1988). "Behavior as an early indicator of pesticide toxicity”. Toxicol. Ind. Health, 3: 351-360.

Wing, K.D., Glickman, A.H., and Casida, J.E. (1983). "Oxidative Bioactivation of S-alkyl Phosphorothiolate Pesticides: Stereospecificity of Profenofos Insecticide Activation”. Science, 219: 63-65.

WHO (1986). Environmental Health Criteria 63. Organophosphorus Insecticides: A General Introduction.

World Health Organization, Geneva.