Reversal of Cadmium-induced Oxidative Stress in Chicken by Herbal Adaptogens Withania somnifera and Ocimum sanctum
Authors
-
Department of Pharmacology and Toxicology, NTR College of Veterinary Science, Gannavaram, Krishna (District) ,IN
K. Bharavi -
Department of Pharmacology and Toxicology, College of Veterinary Science, Rajendranagar ,INA. Gopala Reddy
-
Department of Pharmacology and Toxicology, NTR College of Veterinary Science, Gannavaram, Krishna (District) ,ING. S. Rao
-
Poultry Science, College of Veterinary Science, Rajendranagar, Hyderabad - 30, Andhra Pradesh ,INA. Rajasekhara Reddy
-
Project Directorate on Poultry, College of Veterinary Science, Rajendranagar, Hyderabad - 30, Andhra Pradesh ,INS. V. Rama Rao
Keywords:
Adaptogens, cadmium toxicity, chicken, Ocimum sanctum, Withania somniferaAbstract
The present study was carried out to evaluate the herbal adaptogens Withania somnifera and Ocimum sanctum on cadmium-induced oxidative toxicity in broiler chicken. Cadmium administration at the rate of 100 ppm orally along with feed up to 28 days produced peroxidative damage, as indicated by increase in TBARS, reduction in glutathione (GSH) concentration in liver and kidney, and increase in catalase (CAT) and superoxide dismutase (SOD) of erythrocytes. Herbal adaptogens Withania somnifera roots and Ocimum sanctum leaf powder administration at the rate of 0.1% through feed reversed the antioxidant enzyme of RBC, i.e., CAT and SOD, nonenzymatic antioxidants GSH and lipid peroxidation marker TBARS of liver and kidney. Liver and kidney tissue repair and normal function was assessed by alanine aminotransaminase for liver and creatinine and blood urea nitrogen for kidney. In conclusion, oral administration of Withania somnifera root and Ocimum sanctum leaf powder prevented cadmium-induced peroxidation of tissues.Downloads
Published
How to Cite
Issue
Section
Accepted 2018-05-15
Published 2018-05-16
References
Zhai L, Liao X, Chen T, Yan X, Xie H, Wu B, et al. Regional assessment of cadmium pollution in agricultural lands and the potential health risk related to intensive mining activities. A case study in Cnenzhou city, China. J Environ Sci (China) 2008;20:696-703.
Liao XY, Chen TB, Xie H, Liu YR. Soil as contamination and its risk assessment in areas near the industrial districts of chenzhou city, Southern china. Environ Int 2005;31:791-8.
Satarug S, Baker JR, Urbenjapol S, Haswell-Elkins M, Reilly PE, Williams DJ, et al. A global perspective on cadmium pollution and toxicity in non-occupationally exposed population. Toxicol Lett 2003;137:65-83.
Akyolcu MC, Ozcelik D, Dursun S, Toplan S and Kahraman R. Accumulation of cadmium in tissue and its effect on live performance. J Phys IV France 2003;107:33.
Uyanik F, Even M, Atasever A, Tuncoku G, Kolsuz AH. Changes in some Biochemical Parameters and organs of broilers exposed to cadmium and effect of zinc on cadmium induced alterations. Israel J Vet Med 2001;56:128-34.
Heyno E, Klose C, Krieger-Liszkay A. Origin of cadmium-induced reactive oxygen species production: mitochondrial electron transfer versus plasma membrane NADPH oxidase. New Phytol 2005. In Press.
Quig D. Cysteine metabolism and metal toxicity. Altern Med Rev 1998;3:262-70.
Bagchi D, Vuchetich PJ, Bagchi M, Hassoun EA, Tran MX, Tang L, et al. Induction of oxidative stress by chronic administration of sodium dichromate (chromium vi) and cadmium chloride (cadmium II) to rats. Free Radic Biol Med 1997;22:471-8.
Kara H, Karatas F, Canatan H, Serv K. Effect of exogenous metallothionein on acute cadmium toxicity in rats. Biol Trace Elem Res 2005;104:223-32.
Stohs SJ, Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med 1994;18:321-36.
Kaczmarski M, Wojicicki J, Samochowiee L, Dutkiewicz T, Sych Z. The influence of exogenous antioxidants and physical exercise on some parameters associated with production and removal of free radicals. Die Pharmazie 1999;54:303-6.
Kulkarni SK, Dhir A. Withania Somnifera: An Indian ginseng. Prog Neuropsychopharmacol Biol Psychiatry 2008;32:1093-105.
Mishra LC, Singh BB, Dagenais S. Scientific basis for the therapeutic use of Withania somnifera (Aswagandha): A review. Altern Med Rev 2000;5:334-46.
Sankar SR, Manivasagam T, Krishnamurt A, Ramanathan M. The neuroprotective effect of Withania somnifera root extract in MPTP-intoxicated mice: An analysis of behavioral and biochemical variables. Cell Mol Biol Lett 2007;12:473-81.
Prakash P, Gupta N. Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian J Physiol Pharmacol 2005;49:125-31.
Monica B, Gupta RY. Effect of ocimum sanctum (Tulsi) leaf powder on clinicopathological and immune response in chicken. Hariyana Vet 2004;43:48-50.
Panossian AG. Adaptogens: Toxic herbs for fatigue and stress. Altern Ther Health Med 2003;9:327-31.
Calliborne AL. Assay of catalase Hand book of oxygen Radical Research; In: Greenwald RA, Editor. Baco-Raton: CRC Press; 1985.
Markulund S, Markulund G. Involvement of the superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. J Comp Physiol B 1974;47: 469-74.
Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S transferase in rat lung and liver. Biochem Biophys Acta 1979;582:67-8.
Subramanian KA, Manohar M, Mathan VI. An unidentified inhibitor of lipid peroxidation in intestinal mucosa. Biochim Biophys Acta 1988;962:51-8.
Bergmeyer HU, Horder M, Rej R. Approved recommendation (1986) Approved recommendation (1985) on IFCC methods for the measurement of catalytical concentration of enzyme, part 3. IFCC for alanine aminotransferase. J Clin Chem Clin Biochem 1986;24:481-9.
Apple F, Bandt C, Prosch A, Erlandson G, Holmstrom V, Scholen J, et al. Creatinine clearance: enzymatic vs Jaffe determinations of creatinine in plasma and urine. Clin Chem 1986;32:388-90.
Wybenga DR, Di Giorgio J, Pileggi VJ. Manual and automated methods for urea nitrogen measurement in whole serum. Clin Chem 1971;17:891-5.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
Liochev SI. The mechanism of "Fenton-like reactions and their importance for biological systems. In: Sigel A, Sigel H, Editors. A biologists' view. In Metal Ions in Biological Systems. USA: Marcel Dekker Inc; 1999. p. 1-39.
Goyer RA, Clarkson TW. Toxic effects of metals. Casarett and Doull's Toxicology. The basic Science of Poisoning. 6th ed, Vol-24. 2001. p. 811-68.
Wim W. Detmar B. Cadmium induced apaptosis in C6 glioma cells: Influence of oxidative stress. Biometals 2004;17:65-78.
Sies H. Strategies of antioxidant defense Eur J Biochem 1993;215:213-9.
Davis L, Kuttan G, Kuttan G. Effect of Withania somnifera on dibenzyl benzanthracene (DMBA) induced carcinogenesis. J Ethanopharmacol 2001;75:165-8.
Prakash J, Gupta SK, Dinda AK. Withania somnifera root extract prevents DMBA induced squamous cell carcinoma of skin in swiss albino mice. Nutr Cancer 2002;42:91-7.
