Acetaminophen-induced Hepato- and Nephrotoxicity and Amelioration by Silymarin and Terminalia chebula in Rats


  • College of Veterinary Science, Hyderabad, Department of Pharmacology and Toxicology, Andhra Pradesh, 500030, India


Experimental study was conducted to evaluate the hepato- and renoprotective effect of silymarin and Terminalia chebula against experimentally-induced acetaminophen (APAP) toxicity in rats. Oral administration of APAP @ 500 mg/kg for 1 to 3 days to all the four groups (six rats in each) resulted in significant elevation of serum triglycerides, total cholesterol, blood urea nitrogen, serum creatinine, and aspartate transaminase activity. Posttreatment with silymarin @ 25 mg/kg and T. chebula 125 mg/kg in groups 2 and 3 and their combination to group 4 from day 4 to 14 has significantly reversed the alterations of above said markers and offered better protection. The results of the study enunciated that silymarin and T. chebula exhibit good hepato- and nephro-protection against APAP toxicity.


Acetaminophen, hepatotoxicity, nephrotoxicity, silymarin, Terminalia chebula

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Wogan GN. The induction of liver cell cancer by chemical. In: Cameron HM, Linsell DH, Warwick GP, Editors. Liver cell cancer. Amsterdam: Elsevier scientific; 1976. p. 21-52.

Letteron P, Labbe G, Degott C, Berson A, Fromentry B, Decaforge M, et al. Mechanism of protective effects of silymarin against carbontetrachloride-induced lipid peroxidation and hepatotoxicity in mice. Biochem Pharmacol 1990;39:2027-34.

Kirtikar KR, Basu BD. Terminalia chebula In: Indian Medicinal Plants. 2nd ed. Allahabad: India Lolit Mohan Basu Publication; 1935. p. 1020-3.

Prasad L, Husain Khan T, Jahangir T, Sultana S. Chemomodulatory effects of Terminalia chebula against nickel chloride induced oxidative stress and tumor promotion response in male Wistar rats. J Trace Elem Med Biol 2006;20:233-9.

Zaher A, Hady H, Mahmoud M, Farrag M. The potential protective role of alpha-lipoic acid against acetaminophen-induced hepatic and renal damage. Toxicol 2008;243:261-70.

Richie JP Jr, Long CA, Chen TS. Acetaminophen-induced depletion of glutathione and cysteine in aging mouse kidney. Biochem Pharmacol 1992;44:129-35.

Kaneko JJ, Harvey JW, Michael LB. Clinical Biochemistry of Domestic Animals. 5th ed. New York: Academic Press; 1997. p. 182-9, 857-79.

Lee HS, Won NH, Kim KH, Lee H, Jun W, Lee KW. Antioxidant effects of aqueous extract of Terminalia chebula in vivo and in vitro. Biol Pharmaceutical Bull 2005;28:1639-44.

Rao NK, Nammi S. Antidiabetic and renoprotective effects Terminalia chebula Retz. Seeds in streptozotocin-induced diabetic rats. BMC Complement Altern Med 2006;6:17.

Deák G, Müzes G, Láng I, Nékám K, González-Cabello R, Gergely P, et al. Effects of two bioflavanoids on certain cellular immune reactions. Acta Physiol Hung 1990;76:113-21.


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