A total of 225 male broiler chicks (Cobb strain) of day-old age were procured for the study. The chicks were randomly divided into 15 groups consisting of 15 chicks in each group. Group 1 was maintained as basal diet control and group 2 on chlorpyrifos (CPS) at 100 ppm in feed throughout 6 wk as iron toxic control without any treatment. Groups 3–15 were maintained on CPS at 100 ppm in feed for the 4 wk (28 days) of study and thereafter administered with different herbs and their combinations for remaining 2 wk. The blood samples were drawn from wing vein on 28th day and 42ndd day from the birds in each group for the estimation of lipid and protein profiles. The birds were sacrificed at the end of 6th week and liver tissues were collected for histological examination. The concentrations of total cholesterol, low density lipoprotein (LDL) cholesterol, triglycerides, total protein, albumin and globulins and the A/G ratio were increased significantly (P<0.05) in toxic groups (2–15), while high density lipoprotein (HDL) cholesterol was significantly (P<0.05) decreased at the end of 4th week. However, following supplementation of herbs and herbal combinations, the values of lipid and protein profile in groups 3–15 revived toward normal at the end of 6th week. Histopathology of liver in CPS toxic control (group 2) revealed areas of degeneration, while groups 3–15 that were treated with herbs and their combinations exhibited these changes in a milder form, indicating regenerative alterations. The study revealed that chorpyrifos-induced changes in lipid and protein profile were improved by supplementation of certain herbs.
Broilers, chlorpyrifos, lipid profile, protein profile
Goel A, Dani V, Dhawan DK. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos induced toxicity. Chem Biol Interact 2005;156:131-40.
Farrukh A, Iqbal A, Zafar M. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants Turkish J Biol 2006;30:177-83.
Kaneko JJ, Harvey JW, Michael LB. Clinical Biochemistry of Domestic Animals. 5th ed., New York: Academic Press; 1997.
Qureshi AA, Din ZZ, Abuirmeileh N, Burger WC, Ahmad Y, Elson CE. Suppression of avian hepatic lipid metabolism by solvent. extracts of garlic: Impact on serum lipids. J Nutr 1983;113:1746-55.
Iwata I. Effects of spirulina on plasma lipoprotein lipase activity in rats. J Nutr Sci Vitaminol 1990;36:165-71.
Vinuthan MK, Girish Kumar V, Narayanaswamy M, Veena T. Lipid lowering effect of aqueous leaves extract of Murraya koenigii (Curry leaf) on alloxan induced male diabetic rats. Pharmacog Mag 2007;3:112-115
Shigemastsu N, Asano R, Shimosaka M, Okazaki M. Administration with the extract of Gymnema sylvestre R. Br leaves on lipid metabolism in rats. Biol Pharmaceutical Bull 2001;24: 713-7.