Aldrin Induce Acute Toxicity at Enzymatic and m-RNA Expression Levels in Zebrafish


Affiliations

  • Gujarat Forensic Sciences University, Laboratory of Analytical and Molecular Toxicology (Forensic Chemistry and Toxicology Laboratory), Institute of Forensic Science, Gandhinagar, Gujarat, 382007, India

Abstract

Aldrin is a systematic toxicant, belongs to cyclodiene class of organochlorine pesticide and banned due to its toxic, bioaccumalative and persistent nature but still detected from environmental components. Present study includes the assessment of aldrin toxicity at two sublethal concentrations in zebrafish. A total 81 zebrafish were divided into three groups viz. group 1: Control, group 2 and 3: Exposed groups which were given 3 mg/mL and 6 mg/mL of aldrin, respectively for 24 hours. The markers of oxidative stress viz. antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase) were examined in terms of biochemical activities and gene expression. Acute exposure of aldrin (both concentrations) caused significant alteration in antioxidant enzymes activities and gene expression in liver, kidney and brain tissues, which were more prominent in brain in concentration dependent manner. Study provides a baseline data to understand alterations in enzymatic activity and expression leading to toxic manifestation of aldrin in aquatic animals.

Keywords

Aldrin, Gene Expression, Organochlorine, Oxidative Stress, Zebrafish.

Subject Discipline

Toxicology

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References

Jhamtani RC, Shukla S, Dahiya MS, Agarwal R. Forensic toxicology research to investigate environmental hazard. JFSCI. 2017a; 3(2): 001–4.

Jhamtani RC, Shukla S, Dahiya MS, Agarwal R. Impact ofco-exposure of aldrin and titanium dioxide nanoparticles at biochemical and molecular levels in zebrafish. EnvironToxicol Pharmacol. 2018; 58: 141–55. PMid: 29331773. https://doi.org/10.1016/j.etap.2017.12.021

Costa LG. The neurotoxicity of organochlorine and pyrethroidpesticides. Lotti M, Bleecker ML. Eds. Occupational Neurology: Handbook of Clinical Neurology. 2015; 131(3):135–48. https://doi.org/10.1016/B978-0-444-626271.00009-3

Arant FS. Toxicty of aldrin to chickens. J Econ Ent, 1952;45: 121. https://doi.org/10.1093/jee/45.1.121

Fitzhugh OG, Nelson AA, Quaife ML. Chronic oral toxicityof aldrin and dieldrin in rats and dogs. Fd Cosmet Tox. 1964;2: 551. https://doi.org/10.1016/S0015-6264(64)80354-0

Lamai SL, Warner GF, Walker CH. Effects of dieldrin on lifestages of the African catfish, Clarias gariepinus (Burchell). Ecotoxicol Environ Saf. 2001; 42: 22–9. PMid: 9931234. https://doi.org/10.1006/eesa.1998.1723

Ressang AA, Titus I, Andar RS, Soedarmo D. Aldrin, dieldrin and endrin intoxication in cats. Comm Vet. 1958;2: 71–88.

Bachowski S, Kolaja KL, Xu Y, Ketcham CA, StevensonDE, Walborg EF, Klaunig JE. Role of oxidative stress in the mechanism of dieldrin’s hepatotoxicity. Ann Clin Lab Sci. 1997; 27(3): 196–209. PMid: 9142372.

Jhamtani RC, Shukla S, Dahiya MS, Agarwal R. Evaluationof acute effects of lead at sub-lethal concentrations inzebrafish. Res J Environ Toxicol. 2017b; 11: 97–103. https://doi.org/10.3923/rjet.2017.97.103

Agarwal R, Raisuddin S, Tewari S, Goel SK, Raizada RB,Behari JR. Evaluation of comparative effect of pre‐and post-treatmentof selenium on mercury‐induced oxidative stress, histological alterations and metallothione in mRNA expressionin rats. J Biochem Mol Toxicol. 2010; 24(2): 123–35. PMid: 20143455.

Shukla S, Jhamtani RC, Dahiya MS, Agarwal R. Oxidative injury caused by individual and combined exposureof neonicotinoid, organophosphate and herbicide inzebrafish. Toxicol Rep. 2017a; 4: 240–4. PMid: 28959645PMCid: PMC5615116. https://doi.org/10.1016/j.toxrep.2017.05.002

Slotkin AT, Seidler FJ. Oxidative and excitory mechanisms of developmental neurotoxicity: Transcriptional profiles forchlorpyrifos, diazinon, dieldrin and divalent nickel in PC12cells. Environ Health Perspect. 2009; 117(4): 587–96. PMid:19440498 PMCid: PMC2679603. https://doi.org/10.1289/ehp.0800251

Jhamtani RC, Shukla S, Dahiya MS, Agarwal R. Assessment of environmental hazards caused by industrial effluent discharge using zebrafish as an aquatic biomarker. Curr TopicsToxicol. 2017c; 13: 105–12.

Brammell BF, Wigginton AJ. Differential gene expression in zebrafish (Danio rerio) following exposure to gaseous diffusion plant effluent and effluent receiving stream water. Ame J Environ Stud. 2010; 6(3): 286–94.

Organisation for economic co-operation and development. OECD guidelines for the testing of chemicals, Section 2. Effects on biotic systems; 2004.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Ind J Biochem Biophys. 1984; 21(2): 130–2. PMid: 6490072.

Sinha AK. Colorimetric assay of Catalase. Anal Biochem. 1972; 47: 389–94. https://doi.org/10.1016/00032697(72)90132-7

Flohe L, Gunzler WA. Assays of glutathione peroxidase. Meth. Enzymol. 1984; 105(1): 114–21. https://doi.org/10.1016/S0076-6879(84)05015-1

Lowry HW, Rosebrough NJ, Farr AL, Randall RJ, Protein measurement with folin phenol reagent. J Biol Chem. 1951;193(1): 265–75. PMid: 14907713.

Pfaffl MW. A new mathematical model for relative quantificationin real-time RT–PCR. Nucl Acids Res. 2001; 29(9):e45. PMid: 11328886 PMCid: PMC55695. https://doi.org/10.1093/nar/29.9.e45

Shukla S, Jhamtani RC, Dahiya MS, Agarwal R. Anovel method to achieve high yield of total RNA from zebrafish for expression studies. Int J Bio. 2017b;6(05): 5383–5.

Banerjee BD, Seth V, Ahmed RS. Pesticide-induced oxidativestress: Perspectives and trends. Rev Environ Health. 2001; 16: 1–40. PMid: 11354540. https://doi.org/10.1515/REVEH.2001.16.1.1

Shao B, Zhu L, Dong M, Wang J, Wang J, Xie H, ZhangQ, Du Z. and Zhu S. DNA damage and oxidative stress induced by endosulfan exposure in zebrafish (Danio rerio). Ecotox. 2012; 21: 1533–40. PMid: 22535316. https://doi.org/10.1007/s10646-012-0907-2

Sakuragui MM, Paulino MG, Pereira CDS, Carvalho CS,Sadauskas-Henrique, Fernandes MN. Integrated use of antioxidant enzymes and oxidative damage in two fish species to assess pollution in man-made hydroelectric reservoirs. Environ Poll. 2013; 178: 41–e51. PMid: 23542355. https://doi.org/10.1016/j.envpol.2013.02.032

Kitazawa M, Anantharam V, Kanthasamy AG. Dieldrin induced oxidative stress and neurochemical changes contribute to apoptopic cell death in dopaminergic cells. Free Radic Biol Med. 2002; 31(11): 1473–85. https://doi.org/10.1016/S0891-5849(01)00726-2

Kim BM, Lee JW, Seo JS, Shin KH, Rhee JS, Lee JS. Modulat edexpression and enzymatic activity of the monogonon trotifer Brachionus koreanus Cu/Zn- and Mn-superoxidedismutase (SOD) in response to environmental biocides. Chemosphere. 2015; 120: 470–8. PMid: .25260044 https://doi.org/10.1016/j.chemosphere.2014.08.042

Jamaluddin S, Poddar MK. Role of brain regional GABA:Aldrin-induced stimulation of locomotor activity in rat. Neurochem Res. 2001; 26(4): 439–51. PMid: 11495356. https://doi.org/10.1023/A:1010915518292

Bano Y. Effects of aldrin on serum and liver constituents of freshwater catfish Clarius batracus L. Proc Indian Acad Sci (Anim Sci.). 1982; 91(1): 27–32.

Choi SI, Kim KS, Kim BY, Ahn SY, Cho HJ, Lee HK,Cho HS, and Kim EK. Decreased catalase expression andincreased susceptibility to oxidative stress in primary cultured corneal fibroblasts from patients with granular corneal dystrophy type II. Ame J Pathol. 2009; 175(1):248–61. PMid: 19497990 PMCid: PMC2708811 https://doi.org/10.2353/ajpath.2009.081001

Hansen BH, Rømma S, Garmo ØA, Olsvik PA, AndersenRA. Antioxidative stress proteins and their gene expression in brown trout (Salmo trutta) from three rivers with different heavy metal levels. Compar Biochem Physiol C ToxicolPharmacol. 2006; 143(3): 263–74. PMid: 16616685. https://doi.org/10.1016/j.cbpc.2006.02.010

Karacca M, Varışlı L, Korkmaz K, Özaydın O, Percin F,Orhan H. Organochlorine pesticides and antioxidant enzymes are inversely correlated with liver enzyme gene expression in Cyprinus carpio. Toxicol Lett. 2014; 230(2):198–207. PMid: 24583044. https://doi.org/10.1016/j.toxlet.2014.02.013

Ahmad I, Hamid T, Fatima M, Chand HS, Jain SK, AtharM, Raisuddin S. Induction of hepatic antioxidants in freshwater catfish (Channa punctatus Bloch) is a biomarker of paper mill effluent exposure. Biochim Biophy Acta (BBA)Gen Sub. 2000; 1523(1): 37–48. https://doi.org/10.1016/S0304-4165(00)00098-2

Bainy AC, Arisi AC, Azzalis LA, Simizu K, Barros SB,Videla LA, Junqueira VB. Differential effects of short‐term lindane administration on parameters related to oxidativestress in rat liver and erythrocytes. J Biochem Mol Toxicol.1993; 8(4): 187–94. https://doi.org/10.1002/jbt.2570080404

Jin Y, Zhang X, Shu L, Chen L, Sun L, Qian H, Liu W, FuZ. Oxidative stress response and gene expression withatrazine exposure in adult female zebrafish (Danio rerio). Chemosphere. 2010; 78(7): 846–52. PMid: 20036412.https://doi.org/10.1016/j.chemosphere.2009.11.044

Liu M, Liang Y, Chigurupati S, Lathia JD, Pletnikov M, SunZ, Crow M, Ross CA, Mattson MP, Rabb H. Acute kidneyi njury leads to inflammation and functional changes in the brain. Journal of the Ame Soc Nephrol. 2008; 19(7):1360–70. PMid: 18385426 PMCid: PMC2440297. https://doi.org/10.1681/ASN.2007080901

Ikeda T, Murata Y, Quilligan EJ, Parer JT, MurayamaT, Koono M. Histologic and biochemical study ofthe brain, heart, kidney and liver in asphyxia caused by occlusion of the umbilical cord in near-term fetal lambs. Ame J Obstet Gynecol. 2000; 182(2): 449–57. https://doi.org/10.1016/S0002-9378(00)70238-9


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