Protective Effects of Dioscorea alata L. in Aniline Exposure"‘Induced Spleen Toxicity in Rats: A Biochemical Study

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Authors

  • Department of Pharmacology, Shri Neminath Jain Brahmacharyashram's, Shriman Suresh Dada Jain College of Pharmacy, Neminagar, Chandwad, Nashik, Maharashtra ,IN
  • Department of Pharmacology, Shri Neminath Jain Brahmacharyashram's, Shriman Suresh Dada Jain College of Pharmacy, Neminagar, Chandwad, Nashik, Maharashtra ,IN
  • Department of Pharmacology, Shri Neminath Jain Brahmacharyashram's, Shriman Suresh Dada Jain College of Pharmacy, Neminagar, Chandwad, Nashik, Maharashtra ,IN

Keywords:

Aniline, antioxidants, dioscorea alata, spleen toxicity
Pharmacology

Abstract

Introduction: Present study was designed to evaluate the protective effects of ethanolic extract of Dioscorea alata L. (DA) on hematological and biochemical changes in aniline"‘induced spleen toxicity in rats.Dioscorea alata L. (DA) on hematological and biochemical changes in aniline"‘induced spleen toxicity in rats. Materials and Methods: Wistar rats of either sex (200–250g) were used in the study and each group contains six rats. Splenic toxicity was induced in rats by administration of aniline hydrochloride (AH; 100 ppm) in drinking water for a period of 30 days. Treatment groups received DA (50 and 100 mg/kg/day, po) along with AH. At the end of treatment period, various serum and tissue parameters were evaluated. Result: Rats administered with AH (100 ppm) in drinking water for 30 days showed a significant alteration in general parameters (organ weight, body weight, water intake, feed consumption, and fecal matter content), hematological parameters (red blood cell ( RBC), white blood cell (WBC), and hemoglobin content), and biochemical parameters (total iron content, lipid peroxidation, reduced glutathione (GSH), and nitric oxide (NO) content) of spleen. Treatment with DA (50 and 100 mg/kg/day, po) for 30 days along with AH showed significant recovery in aniline"‘induced splenic toxicity. Conclusion: The present result showed that involvement of oxidative and nitrosative stress in aniline"‘induced splenic toxicity and DA protects the rats from the toxicity, which might be due to its antioxidant property and the presence of different phytochemicals.

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Published

2018-06-04

How to Cite

Khan, R., Upaganlawar, A. B., & Upasani, C. (2018). Protective Effects of <i>Dioscorea alata L.</i> in Aniline Exposure"‘Induced Spleen Toxicity in Rats: A Biochemical Study. Toxicology International, 21(3), 294–299. Retrieved from http://informaticsjournals.com/index.php/toxi/article/view/21414

Issue

Section

Original Research
Received 2018-06-04
Accepted 2018-06-04
Published 2018-06-04

 

References

Khan MF, Boor PJ, GU Y, Alcock NW, Ansari GA. Oxidative stress in the splenotoxicity of aniline. Fundam Appl Toxicol 1997;35:22"‘30.

Firoze Khan M, Wu X, Wang J. Up"‘regulation of transforming factor"‘ β1 in the spleen of aniline"‘treated rats. Toxicol Appl Pharmacol 2003;187:22"‘8.

Khan MF, Kannan S, Wang J. Activation of transcription factor AP"‘1 and mitogen"‘activated protein kinases in aniline"‘induced splenic toxicity. Toxicol Appl Pharmacol 2006;210:86"‘93.

Pauluhn J. Subacute inhalation toxicity of aniline in rats: Analysis of time"‘dependence and concentration"‘dependence of hematotoxic and splenic effects. Toxicol Sci 2004;81:198"‘215.

Bus JS, Popp JA. Perspectives on the mechanism of action of the splenic toxicity of aniline and structurally related compounds. Food Chem Toxicol 1987;25:619"‘26.

Miyoshi N, Nagasawa T, Mabuchi R, Yasui Y, Wakabayashi K, Tanaka T, et al. Chemoprevention of azoxymethane/dextran sodium sulfate"‘induced mouse colon carcinogenesis by freezed"‘dried yam sanyaku and its constituents diosgenin. Cancer Prev Res (Phila) 2011;4:924"‘34.

Atindehou KK, Kone M, Terreaux C, Traore D, Hostettmann K, Dosso M. Evaluation of the antimicrobial potential of medicinal plants from the Ivory Coast. Phytother Res 2002;16:497"‘502.

Liu YH, Lin YS, Liu DZ, Han CH, Chen CT, Fan M, et al. Effect of different types of yam (Dioscorea alata) products on the blood pressure of spontaneously hypertensive rats. Biosci Biotechnol Biochem 2009;73:1371"‘6.

Farombi EO, Britton G, Emerole GO. Evaluation of the antioxidant activity and partial characterization of extracts from browned yam flour diet. Food Res Int 2000;33:493"‘9.

Lee SC, Tsai CC, Chen JC, Lin CC, Hu ML, Lu S. The evaluation of reno"‘ and hepatoprotective effects of huai"‘shan"‘yao (Rhizome Dioscoreae). Am J Chin Med 2002;30:609"‘16.

Dykman KD, Tone C, Ford C, Dykman RA. The effect of nutritional supplement on the symptoms of fibromyalgia and chronic fatigue syndrome. Integr Physiol Behav Sci 1998;33:61"‘71.

Godkar PB, Godkar DP. Determination of Hemoglobin. Text Book of Medical Laboratory Technology. 2nd ed. Mumbai, India: Published by Balani Publishing House; 2008. p. 726-31.

Ramsay WN. The determination of total iron"‘binding capacity of serum. Clin Chim Acta 1957;2:221"‘6.

Slater TF, Sawyer BC. The stimulatory effect of carbon tetrachloride and other halogenoalkanes or peroxidative reaction in the rat liver functions in vitro. General features of the systems used. Biochem J 1971;123:805"‘14.

Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S"‘transferase activities in rat lung and liver. Biochem Biophys Acta 1979;582:67"‘78.

Guevara I, Iwanejko J, Dembinska"‘kiec A, Pankiewicz J, Wanat A, Anna P, et al. Determination of nitrite/nitrate in human biological material by the simple Griess reaction. Clin Chim Acta 1998;274:177"‘88.

Khan MF, Wu X, Ansari GA. Contribution of nitrosobenzene to splenic toxicity of aniline. J Toxicol Environ Health A 2000;60:263"‘73.

Lubag A, Laurena C, Mendoza E. Antioxidant of purple and white greater yam (Dioscorea alata L.) varieties from the Philippines. Philippine J Sci 2008;137:61"‘7.

Khan MF, Green SM, Ansari GA, Boor PJ. Phenylhydroxylamine: Role in aniline"‘associated splenic oxidative stress and induction of subendocardial necrosis. Toxicol Sci 1998;42:64"‘71.