Antioxidant Properties of the Extracts of Talinum triangulare and its Effect on Antioxidant enzymes in Tissue Homogenate of Swiss Albino Rat

Jump To References Section

Authors

  • ,NG
  • Department of Biochemistry, Ekiti State University, Ado"‘Ekiti ,NG

Keywords:

Antioxidant, free radicals, homogenate, in vitro, Talinum triangulare
Biochemistry

Abstract

Objectives: This study was designed to put into consideration both the in vitro and in vivo investigations on Talinum triangulare (Tt), an herbaceous perennial plant that is a native of tropical America and one of the most important vegetables in Nigeria. Methods: Total phenolic contents in (mg GAE/100 g), flavonoid contents, the ferric reducing antioxidant properties (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl free radical scavenging ability (OH-) and iron chelating ability were carried out in vivo using standard described methods while GSH, GPx, catalase and SOD were determined in vivo using standard described methods. Results: In the three different solvents extraction of T. triangulare that were studied in vitro, it was noted that ethyl acetate and ethanolic fractions of T. triangulare showed potent antioxidant activity against DPPH and iron chelating property with high phenolic content except Hydroxyl free radical scavenging ability that showed highest value in the aqueous extract, while the Reduced GSH indicated the highest in the parameter determined in vivo. Conclusion: The antioxidant properties showed in this solvent extractable component probably could have been the basis for the enhanced activities of antioxidant enzymes at very lower dose in the examined tissue homogenates. Therefore, T. triangulare can thereby serve as a means of Preventing some of major degenerative diseases challenging Humans.

Downloads

Download data is not yet available.

Published

2018-06-04

How to Cite

Afolabi, O. B., & Oloyede, O. I. (2018). Antioxidant Properties of the Extracts of <i>Talinum triangulare</i> and its Effect on Antioxidant enzymes in Tissue Homogenate of Swiss Albino Rat. Toxicology International, 21(3), 307–313. Retrieved from http://informaticsjournals.com/index.php/toxi/article/view/21416

Issue

Section

Original Research
Received 2018-06-04
Accepted 2018-06-04
Published 2018-06-04

 

References

Olajire AA, Azeez L. Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables. Afr J Food Sci Tech 2011;2:22"‘9.

Oboh G, Akinyemi AJ, Ademiluyi AO. Inhibition of α"‘amylase and α– glucosidase activities by ethanolic extract of Telfairiaoccidentalis (fluted pumpkin) leaf. Asian Pac J Trop Biomed 2012;2:733"‘8.

Afolabi OB, Oyeyemi AO, Obafemi TB, Awe JO. Phytochemical Screening of the Bark of Vernonia Amygdalina. J Nat Sci Res 2014;7:49"‘50.

Oloyede OI, Aluko OM. Determination of antioxidant potential of Momordica Foetida leaf extract on tissue homogenate. Sci J Med Clin Trial 2012;4:225.

Bahorun T, Soobrattee MA, Lux, Mon"‘Ramma V, Aruoma OI. Free radicals and antioxidants in cardiovascular health and diseases. Int J Medupdates 2006;1:25"‘41

Alia M, Horcajo C, Bravo L, Goya L. Effect of grape antioxidant dietary fiber on the total antioxidant capacity and the activity of liver antioxidant enzymes in rats. Nutr Res 2003;23:1251"‘67.

Oboh G, Rocha GBT. Antioxidant in foods: A new challenge for food processors: In: Panglossi HV, editor. Leading edge antioxidants research. Nova Science Publishers Inc: New York; 2007. pp. 35–64.

Valko M, Leibfritz D, Moncola J, Cronin MD. Free radicals and antioxidants in normal physiological functions and human disease. Rev Int J Biochem Cell Biol 2007;39:44"‘84.

Droge W. Free radicals in the physiological control of cell function. Physiol Rev 2002;82:47"‘95.

Pham"‘Huy LA, He H, Pham"‘Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4:89"‘96.

Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol 2001;54:176"‘86.

Minotti G, Aust SD. The requirement for iron (III) in the initiation of lipid peroxidation by iron (II) and hydrogen peroxide. J Biol Chem 1987;262:1098"‘104.

Gyamfi MA, Yonamine M, Aaniya Y. Free radical scavenging action of medicinal herbsfrom Ghana. Thonningia sanguine on experimentally induced liver injuries. Gen Pharmacol 1999;32:661"‘7.

Pulido R, Bravo L, Sauro"‘Calixo F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem 2000;48:3396"‘402.

Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Japanese J Nutr 1986;44:307"‘15.

Singleton VL, Orthofer R, Lamuela"‘Raventós RM. Analysis of total phenols and other oxi"‘ dation substrates and antioxidants by means of Follin"‘Ciocalteu reagent. Met Enzymol 1999;299:152"‘78.

Bao JY, Cai M, Sun G, Wang G, Corke H. Anthocyanins, flavonoid and free radical scavenging activity of myrialrubia extracts and their colour properties and stability. J Agric Food Chem 2005;53:2327"‘32.

Jollow DJ, Mitchell JR, Zampaglione N, Gillete JR. Bromobenzene"‘induced liver necrosis, Protective role of glutathione and evidence for 3,4"‘bromobenzene oxide as the hepatotoxic metabolite. Pharmacol 1974;11:151"‘69.

Rotruck JT, Pope AL, Ganther HE, Swanson DG, Hafeman DG, Schneider C. Chemistry and biology of vitamin E. Mol Nutr Food Res 2005;49:7"‘30.

Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972; 247:3170"‘5.

Beers RF Jr, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 1952; 195:133"‘40.

Zar JH. Biostatistical analysis. 2nd ed. Englewood Cliffs: Prentice"‘Hall; 1984. p. 718.

Amic D, Davidovic"‘Amic D, Beslo D, Trinajstic N. Structure"‘radical scavenging activity relationship of flavonoids. Croat Chem Acta 2003; 76:55"‘61.

Halliwell B, Gutteridge JM. Free radicals in biology and medicine. 2nd ed. Oxford: Clarendon press; 1989. p. 188"‘276.

Meister A, Anderson ME. Glutathione. Ann Rev Biochem 1983; 52:711"‘60.

Vani ML, Reddy KP. Effects of fluoride accumulation on some enzymes of brain and gastrocnemius muscle of mice. Fluorid 2000; 33:17"‘26.