Synergistic Effect of Mercury and Chromium on the Histology and Physiology of Fish, Tilapia Mossambica (Peters, 1852) and Lates calcarifer Calcarifer (Bloch, 1790)


Affiliations

  • Annamalai University, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences,, Parangipettai
  • Annamalai University, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences,, Parangipettai, Tamil Nadu, India

Abstract

Fingerlings of estuarine fishes, Tilapia mossambica and Lates calcarifer were exposed to sub-lethal concentration of mercury and chromium (2.8 ppm) for a period of 28 days. When these fish were exposed to metals concentration, severe gills alterations were observed. But the alteration was less in fish T. mossambica when compared to that of L. calcarife. The fish L. calcarifer exposed to mercury plus chromium, showed lifting up of the epithelium, swelling, hyperplasia, hypertrophy, proliferation of chloride cells, but in mercury treatment, lamellar fusions, fused secondary lamella and necrosis were observed, whereas in T. mossambica the gills disintegration of epithelial cells, desquamated epithelium, hemorrhaged and exhibited complete damage of epithelial cells of lamellae. The Na+, K+-ATPase activity of both gills and plasma showed significant reduction throughout the experiment period in both fishes. The enzyme activity was more drastic in the case of plasma. The results are discussed in relation to the significance of the above enzyme as non-specific biomarkers against environmental stress.

Keywords

Hg and Cr, histopathology, K+-ATPase, Na+, T. mossambica, L. calcarife

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References

Nies DH. Microbial heavy metal resistance. Appl Microbiol Biotechnol 1999;51:730-50.

Wood JM. Biological cycles for toxic elements in the environment.Science 1974;183:1049-52.

Machiwa JF. Heavy metals and organic pollutants in sediments of Dares Salaam harbour prior to dredging in 1999. Tanz J Sci 2000;26:29-46.

Schreck CB, Lorz HW. Stress response of coho salmon (Oncorhynchus kisutch) elicited by cadmium and potential use of cortisol as an indicator of stress. J Fish Res Board 1978;35:1124-9.

Trudel M, Rasmussen JB. Bioenergetics and mercury dynamics in fish: a modeling perspective. Can J Fish Aquat Sci 2006;63: 1890-902.

Marr JCA, Hansenj A, Meyer JS, Cacela D, Podrabsky T, Lipton J, et al. Toxicity of cobalt and copper to rainbow trout: application of a mechanistic model for predicting survival. Aquat Toxicol 1998;43:225-38.

Verma SR, Jain M, Dalela RC. A laboratory study to assess separate and in-combination effects of zinc, chromium and nickel to the fish Mystus vittatus Acta Hydrochim. Hydrobiol 1982;10:23-9.

Nugegorda D, Rainbow PS. Effect of chelating agent (EDTA) on zinc uptake and regulation by palaemon elegans (Crustacea: Decapoda). J Mar Biol Assoc UK 1988;68:25.

Marking LL. Method of assessing additive toxicity of chemical mixtures. Aquat. Toxicol and Hazard Evaluation. In: Mocyer FM, Hamelink JL, editors. American Society for Testing and Materials Memphis Ten Tennessee ASTM Spec Tech Publ 1977;99-108

Marking LL. Aquatic toxicology. Am Soc Testing Mat 1985;1-667.

Brown VM, Jordan DN, Tiler BA. The acute toxicity to rainbow trout of fluctuating concentrations and mixtures of ammoniac, phenol and zinc. J Fish Biol 1969;1:1-9.

Sturla M, Masini MA, Prato P, Grattarola C, Uva B. Mitochondriarich cells in gills and skin of an African lungfish, Protopterus annectens. Cell Tissue Res 2001;303:351-8.

Mallatt J. Fish gill structural changes induced by toxicants and other irritants: A statistical review. Can J Fish Aquat Sci 1985;42:630-48.

APHA, AWWA and WPCF. In: ‘Standard Methods for the Examination of Water and Waste Water’.14thed. Washington, USA: American Public Health Association;1976.

Finney DJ. Statistical Method in Biological Assay. 3rd ed. London: Griffin Press; 1978

Shiosaka T, Okuda H, Fuji S. Mechanism of phosphorylation of thymidine by the culture filtrate of Clostridium perfringnes and rat liver extract. Biochem Biophys Acta 1971;246:171-83.

Pearse AGE. Histochemistry. 3rd ed. Vol. 1. London: Churchill; 1968.

Roberts RJ. The pathophysilolgy and systemic pathology of teleosts, and laboratory methods. On: fish pathology. 1st ed.Lomdom, UK: Bailliere Tindall; 1978. p. 235-46.

Humason GL. Animal tissue techniques. 4th ed. San Francisco, USA: W H, Freeman and Company; 1979. p. 3-63.

Handy and Penrice RD, Handy and WS, Penrice. The influence of high oral doses of mercuric chloride on organ toxicant concentrations and histopathology in Rainbow trout, Oncorhynchus mydiss. Comp Biochem Physiol 1993;106C pp. 717-24

Oliveira Ribeiro CA, Oliveir Ribeiro E, Pelletier WC, et al. Rouleau Comparative gill damages and bioaccumulation of inorganic mercury on tropical and nordic fish. Environ Res 1996; 83A 2000: pp.286-92

Jagoe CH, Haines TA. Alterations in gill epithelial morphology of yearling sunapee trout exposed to acute acid stress. Trans Am Fish Soc 1983;112:689-95.

Tandjung SD. The acute toxicity and histopathology of brook trout, Salvelinus fontinalis. Mitchill exposed to Aluminium in acid water, Ph.D. dissertation. New York, NY: Fordham University; 1982.p.213.

Segner H, Mathaler R, Linnenbach M. Growth, aluminium uptake and mucous cell morphometrics of early life stages of bown trout, Salmo trutta, in low pH water. Environ Biol Sci 1988;21:153-9.

Wood CM, Simons BP, Mount DR, et al. Physiological evidence of acclimation to acid/aluminum stress in adult brook trout (Salvelinus fontinalis) 2. Blood parameters by cannulation. Can J Fish Aquat Sci 1988b;45:1597-605.

Tietge JE, Johnson RD, Bergman HL. Morphometric changes in gill secondary lamellae of brook trout (Salvelinus fontinalis) after long-term exposure to acid and aluminium. Can J Fish Aquatic Sci 1988;45:1643-8.

Peuranen S, Vuroinen PJ, Vuorinen M, et al. Effects of acidity and aluminium on fish gills in laboratory experiments and in the field. In: ‘The Science of the Total Environment’. Elsevier Science Publisers, Amsterdam 1993.p.979-88.

Ingersoll CG, Sanchez DA, Meyer JS, Gulley DD, Tietge JE.Epidermal response to pH, aluminium and calcium exposure in brook trout, Salvelinus fontinalis. Can J Fish Aquat Sci 1990;47:1616-22.

Bernet D, Schmidt H, Meier W, Burkhardt-Holm P, Wahli T.Histopathology in fish: proposal for a protocol to assess aquatic pollution. J Fish Dis 1999;22:25-34.

Wood EM. Definitive diagnosis of fish mortalities. J Water Pollute Control Fed 1960;32:994-9.

Wedemeyer G. The stress of form alin treatm ents in rainbow trout (Salmo gairdneri) and coho salmon (Oncorhynchus kisutch).Res Board Can 1971;28:1899-904.

Morgan M, Tovell PWA. The Structure of the gill of the trout (Salmo gairdneri) (Richardson). Zellforch M ikrosk Anat 1973;142:147-62.

Mayer FLM, Versteeg MJ, Mekee LC, et al. Physiological and nonspecific biomarkers. In: Huggett RJ, Kimerltr RA Jr, Mehrle PM, Bergman HL, editors. Biomarkers- Niochemical, Physiological and Histological Markers of Anthropogenic Stress. Boca Raton, USA: Lewis publishers 1992.p.5-85.

Wastson TA, Baeamish FWH. Effect of zinc on branchial ATPase activity in vivo in rainbow trout, Salmo gairdneri. Comp Biochem Phsiol 1980;66B:77-82.

Kinter WB, Merkens LS, Janicki RH, Guarino AM. Studies on the mechanism of toxicity of DDT and polychlorinated biphenyls (PCBs): disruption of osmoregulation in marine fish. Environ Health Perspect 1972;1:169-73.

Davis PW, Friendhoff JM, Wedemeyer GA. Organochlorine insecticide, herbicide and polychlorinated biphenyl (PCB) inhibition of Na+, K+- ATPase in rainbow trout. Bull Environ Contam Toxicol 1972;8:69-72.

Solomonson IP, Liepkalns UA, Spector AA. Changes in (Na++ K+)-ATPase activity of Ehrlich ascites tumor cells produced by alteration of membrane fatty acid composition. Biochemistry 1976;15:892-7.

Rangaraj N, Kalant H. Interaction of catecholamines and ethanol on the kinetics of rat brain (Na+ + K+)-ATPase. Environ J Pharmacol 1981;70:157-66.

Kunhert PM. Effect of in vivo chromium exposure on Na+, K+ ATPase and Mg2+- ATPase activity in several tissues of rainbow trout, salmo gairdneri. Bull Environ Contam Toxicol 1976;15:383-96.


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