Release and toxicity of dental resin composite

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Authors

  • ,IN
  • ,IN
  • ,IN

Keywords:

Biocompatibility, dental resin composite, release phenomenon, toxicity

Abstract

Dental resin composite that are tooth"‘colored materials have been considered as possible substitutes to mercury"‘containing silver amalgam filling. Despite the fact that dental resin composites have improved their physico"‘chemical properties, the concern for its intrinsic toxicity remains high. Some components of restorative composite resins are released in the oral environment initially during polymerization reaction and later due to degradation of the material. In vitro and in vivo studies have clearly identified that these components of restorative composite resins are toxic. But there is a large gap between the results published by research laboratories and clinical reports. The objective of this manuscript was to review the literature on release phenomenon as well as in vitro and in vivo toxicity of dental resin composite. Interpretation made from the recent data was also outlined.

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Published

2018-08-14

How to Cite

Gupta, S. K., Saxena, P., Pant, V. A., & Pant, A. B. (2018). Release and toxicity of dental resin composite. Toxicology International, 19(3), 225–234. Retrieved from http://informaticsjournals.com/index.php/toxi/article/view/21768

 

References

Sunnegårdh"‘Grönberg K, van Dijken JW, Funegård U, Lindberg A, Nilsson M. Selection of dental materials and longevity of replaced restorations in Public Dental Health clinics in northern Sweden.J Dent 2009;37:673"‘8.

Reichl FX, Seiss M, Kleinsasser N, Kehe K, Kunzelmann KH, Thomas P, et al. Distribution and excretion of BisGMA in guinea pigs. J Dent Res2008;87:378"‘80.

Goldberg M. In vitro and in vivo studies on the toxicity of dental resin components: A review. Clin Oral Investig 2008;12:1"‘8.

Lu Y, Liu T, Li H, Pi G. Histological evaluation of direct pulp capping with a self"‘etching adhesive and calcium hydroxide on human pulp tissue. Int Endod J2008;41:643"‘50.

Stanislawski L, Lefeuvre M, Bourd K, Soheili"‘Majd E, Goldberg M, Périanin A. TEGDMA"‘induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species. J Biomed Mater Res A 2003;66:476"‘82.

Rogalewicz R, Voelkel A, Kownacki I. Application of HS"‘SPME in the determination of potentially toxic organic compounds emitted from resin"‘based dental materials. J Environ Monit 2006;8:377"‘83.

Marquardt W, Seiss M, Hickel R, Reichl FX. Volatile methacrylates in dental practices. J Adhes Dent 2009;11:101"‘7.

Van Landuyt KL, Nawrot T, Geebelen B, De Munck J, Snauwaert J, Yoshihara K, et al. How much do resin"‘based dental materials release? A meta"‘analytical approach. Dent Mater 2011;27:723"‘47.

León BL, Del Bel Cury AA, Rodrigues Garcia RC. Loss of residual monomer from resilient lining materials processed by different methods. Rev Odonto Ciíªnc 2008;23:215"‘9.

Braden M, Causton BE, Clarke RL. Diffusion in water in composite filling materials. J Dent Res 1976;55:730"‘2.

Sideridou I, Achilias DS, Spyroudi C, Karabela M. Water sorption characteristics of light"‘cured dental resins and composites based on Bis"‘EMA/PCDMA. Biomaterials 2004;25:367"‘76.

Ferracane JL. Hygroscopic and hydrolytic effects in dental polymer networks. Dent Mater 2006;22:211"‘22.

Lenz R. Biodegradable polymers. In:Peppas NA, Langer RS, editors. Advances in polymer science (No 107: biopolymers).Berlin: Springer; 1993. p. 1"‘40.

Drummond JL. Degradation, fatigue and failure of resin dental composite materials. J Dent Res 2008;87:710"‘9.

Bettencourt AF, Neves CB, de Almeida MS, Pinheiro LM, Oliveira SA, Lopes LP, et al. Biodegradation of acrylic based resins: A review. Dent Mater 2010;26:e171"‘80.

Jepson NJ, McGill JT, McCabe JF. Influence of dietary simulating solvents on the viscoelasticity of temporary soft lining materials.J Prosthet Dent 2000;83:25"‘31.

Willershausen B, Callaway A, Ernst CP, Stender E. The influence of oral bacteria on the surfaces of resin"‘based dental restorative materials: An in vitro study. Int Dent J 1999;49:231"‘9.

Fano V, Shatel M, Tanzi ML. Release phenomena and toxicity in polymer"‘based dental restorative materials. Acta Biomed 2007;78:190"‘7.

Schmalz G. The biocompatibility of non"‘amalgam dental filling materials. Eur J Oral Sci 1998;106:696"‘706.

Stanislawski L, Daniau X, Lauti A, Goldberg M. Factors responsible for pulp cell cytotoxicity induced by resin"‘modified glass ionomer cements. J Biomed Mater Res 1999;48:277"‘88.

Geurtsen W. Substances released from dental resin composites and glass ionomer cements. Eur J Oral Sci 1998;106:687"‘95.

Geurtsen W. Biocompatibility of resin"‘modified filling materials.Crit Rev Oral Biol Med 2000;11:333"‘55.

Jaffer F, Finer Y, Santerre JP. Interactions between resin monomers and commercial composite resins with human saliva derived esterases. Biomaterials 2002;23:1707"‘19.

Finer Y, Jaffer F, Santerre JP. Mutual influence of cholesterol esterase and pseudocholinesterase on the biodegradation of dental composites. Biomaterials 2004;25:1787"‘93.

Finer Y, Santerre JP. The influence of resin chemistry on a dental composite's biodegradation. J Biomed Mater Res A 2004;69:233"‘46.

Stanislawski L, Soheili"‘Majd E, Perianin A, Goldberg M. Dental restorative biomaterials induce glutathione depletion in cultured human gingival fibroblast: Protective effect of N"‘acetyl cysteine.J Biomed Mater Res 2000;51:469"‘74.

Pearson GJ, Longman CM. Water sorption and solubility of resin"‘based materials following inadequate polymerization by a visible"‘light curing system. J Oral Rehabil 1989;16:57"‘61.

Hansel C, Leyhausen G, Mai UE, Geurtsen W. Effects of various resin composite (co)monomers and extracts on two caries"‘associatedmicro"‘organisms in vitro. J Dent Res 1998;77:60"‘7.

Khalichi P, Cvitkovitch DG, Santerre JP. Effect of composite resin biodegradation products on oral streptococcal growth.Biomaterials 2004;25:5467"‘72.

Bergenholtz G. Evidence for bacterial causation of adverse pulpal responses in resin"‘based dental restorations. Crit Rev Oral Biol Med2000;11:467"‘80.

Bergenholtz G, Cox CF, Loesche WJ, Syed SA. Bacterial leakage around dental restorations: Its effect on the dental pulp. J Oral Pathol 1982;11:439"‘50.

Murray PE, Windsor LJ, Hafez AA, Stevenson RG, Cox CF.Comparison of pulp responses to resin composites. Oper Dent 2003;28:242"‘50.

Murray PE, Hafez AA, Smith AJ, Cox CF. Identification of hierarchical factors to guide clinical decision making for successful long"‘term pulp capping. Quintessence Int 2003;34:61"‘70.

Hashimoto M, Ito S, Tay FR, Svizero NR, Sano H, Kaga M, et al.Fluid movement across the resin"‘dentin interface during and after bonding. J Dent Res 2004;83:843"‘8.

Chen CC, Chen RC, Huang ST. Enzymatic responses of human deciduous pulpal fibroblasts to dental restorative materials.J Biomed Mater Res 2002;60:452"‘7.

Thonemann B, Schmalz G, Hiller KA, Schweikl H. Responses of L929 mouse fibroblasts, primary and immortalized bovine dental papilla"‘derived cell lines to dental resin components.Dent Mater 2002;18:318"‘23.

Issa Y, Watts DC, Brunton PA, Waters CM, Duxbury AJ. Resin composite monomers alter MTT and LDH activity of human gingival fibroblasts in vitro. Dent Mater 2004;20:12"‘20.

Geurtsen W, Leyhausen G. Chemical"‘Biological interactions of the resin monomer triethyleneglycol"‘dimethacrylate (TEGDMA).J Dent Res2001;80:2046"‘50.

Al"‘Hiyasat AS, Darmani H, Milhem MM. Cytotoxicity evaluation of dental resin composites and their flowable derivatives. Clin Oral Inves2005;9:21"‘5.

Atsumi T, Murata J, Kamiyanagi I, Fujisawa S, Ueha T. Cytotoxicity of photosensitizerscamphorquinone and 9"‘fluorenone with visible light irradiation on a human submandibular"‘duct cell line in vitro. Arch Oral Biol 1998;43:73"‘81.

Martins CA, Leyhausen G, Geurtsen W, Volk J. Intracellular glutathione: A main factor in TEGDMA"‘induced cytotoxicity?Dent Mater 2012;28:442"‘8.

Reichl FX, Esters M, Simon S, Seiss M, Kehe K, Kleinsasser N, et al.Cell death effects of resin"‘based dental material compounds and mercurials in human gingival fibroblasts. Arch Toxicol2006;80:370"‘7.

Moharamzadeh K, Van Noort R, Brook IM, Scutt AM. Cytotoxicity of resin monomers on human gingival fibroblasts and Ha Ca T keratinocytes. Dent Mater 2007;23:40"‘4.

Chang MC, Lin LD, Chan CP, Chang HH, Chen LI, Lin HJ, et al.The effect of BisGMA on cyclooxygenase"‘2 expression, PGE2 production and cytotoxicity via reactive oxygen species"‘ and MEK/ERK"‘dependent and "‘independent pathways. Biomaterials 2009;30:4070"‘7.

Aranha AM, Giro EM, Hebling J, Lessa FC, Costa CA. Effects of light"‘curing time on the cytotoxicity of a restorative composite resin on odontoblast"‘like cells. J Appl Oral Sci 2010; 18:461"‘6.

Chang MC, Lin LD, Chuang FH, Chan CP, Wang TM, Lee JJ, et al.Carboxylesterase expression in human dental pulp cells: Role in regulation of BisGMA"‘induced prostanoid production and cytotoxicity. Acta Biomater 2012;8:1380"‘7.

Wisniewska"‘Jarosinska M, Poplawski T, Chojnacki CJ, Pawlowska E, Krupa R, Szczepanska J, et al. Independent and combined cytotoxicity and genotoxicity of triethylene glycol dimethacrylate and urethane dimethacrylate. Mol Biol Rep 2011;38:4603"‘11.

Schweikl H, Spagnuolo G, Schmalz G. Genetic and cellular toxicology of dental resin monomers. J Dent Res 2006;85:870"‘7.

Schweikl H, Schmalz G, Rackebrandt K. The mutagenic activity of unpolymerized resin monomers in Salmonella typhimurium and V79 cells. Mutat Res 1998;415:119"‘30.

Schweikl H, Schmalz G, Spruss T. The induction of micronuclei in vitro by unpolymerized resin monomers. J Dent Res 2001;80:1615"‘20.

Kleinsasser NH, Wallner BC, Harréus UA, Kleinjung T, Folwaczny M, Hickel R, et al. Genotoxicity and cytotoxicity of dental materials in human lymphocytes as assessed by the single cell microgel electrophoresis (comet) assay. J Dent 2004;32:229"‘34.

Kleinsasser NH, Schmid K, Sassen AW, Harréus UA, Staudenmaier R, Folwaczny M, et al. Cytotoxic and genotoxic effects of resin monomers in human salivary gland tissue and lymphocytes as assessed by the single cell microgel electrophoresis (Comet) assay. Biomaterials 2006;27:1762"‘70.

Durner J, Dć™biak M, Bürkle A, Hickel R, Reichl FX. Induction of DNA strand breaks by dental composite components compared to X"‘ray exposure in human gingival fibroblasts. Arch Toxicol 2011;85:143"‘8.

Spagnuolo G, D'Anto V, Cosentino C, Schmalz G, Schweikl H, Rengo S. Effect of Nacetyl"‘ L"‘cysteine on ROS production and cell death caused by HEMA in human primary gingival fibroblasts.Biomaterials 2006;27:1803"‘9.

Volk J, Engelmann J, Leyhausen G, Geurtsen W. Effects of three resin monomers on the cellular glutathione concentration of cultured human gingival fibroblasts. Dent Mater 2006;22:499"‘505.

Becher R, Kopperud HM, Al RH, Samuelsen JT, Morisbak E, Dahlman HJ, et al. Pattern of cell death after in vitro exposure to GDMA, TEGDMA, HEMA and two compomer extracts. Dent Mater 2006;22:630"‘40.

Samuelsen JT, Dahl JE, Karlsson S, Morisbak E, Becher R.Apoptosis induced by the monomers HEMA and TEGDMA involves formation of ROS and differential activation of the MAP"‘kinases p38, JNK and ERK. Dent Mater 2007;23:34"‘9.

Volk J, Leyhausen G, Dogan S, Geurtsen W. Additive effects of TEGDMA and hydrogenperoxide on the cellular glutathione content of human gingival fibroblasts. Dent Mater 2007;23:921"‘6.

Falconi M, Teti G, Zago M, Pelotti S, Breschi L, Mazzotti G. Effects of HEMA on type I collagen protein in human gingival fibroblasts.Cell Biol Toxicol 2007;23:313"‘22.

Schweikl H, Hiller KA, Eckhardt A, Bolay C, Spagnuolo G, Stempfl T, et al. Differential gene expression involved in oxidative stress response caused by triethylene glycol dimethacrylate.Biomaterials 2008;29:1377"‘87.

Spagnuolo G, D'AntòV, Valletta R, Strisciuglio C, Schmalz G, Schweikl H, et al. Effect of 2"‘hydroxyethyl methacrylate on human pulp cell survival pathways ERK and AKT. J Endod2008;34:684"‘8.

Emmler J, Seiss M, Kreppel H, Reichl FX, Hickel R, Kehe K.Cytotoxicity of the dental composite component TEGDMA and selected metabolic by"‘products in human pulmonary cells. Dent Mater 2008;24:1670"‘5.

Imazato S, Horikawa D, Nishida M, Ebisu S. Effects of monomers eluted from dental resin restoratives on osteoblast"‘like cells.J Biomed Mater Res B Appl Biomater2009;88:378"‘86.

Eckhardt A, Gerstmayr N, Hiller KA, Bolay C, Waha C, Spagnuolo G, et al. TEGDMA"‘induced oxidative DNA damage and activation of ATM and MAP kinases. Biomaterials 2009;30:2006"‘14.

Teti G, Mazzotti G, Zago M, Ortolani M, Breschi L, Pelotti S, et al.HEMA down"‘regulates procollagen alpha1 type I in human gingival fibroblasts. J Biomed Mater Res A 2009;90:256"‘62.

Lee DH, Kim NR, Lim BS, Lee YK, Ahn SJ, Yang HC. Effects of TEGDMA and HEMA on the expression of COX"‘2 and i NOS in cultured murine macrophage cells. Dent Mater 2009;25:240"‘6.

Pawlowska E, Poplawski T, Ksiazek D, Szczepanska J, Blasiak J.Genotoxicity and cytotoxicity of 2"‘hydroxyethyl methacrylate.Mutat Res 2010;696:122"‘9.

Urcan E, Scherthan H, Styllou M, Haertel U, Hickel R, Reichl FX.Induction of DNA double"‘strand breaks in primary gingival fibroblasts by exposure to dental resin composites. Biomaterials 2010;31:2010"‘4.

Drozdz K, Wysokinski D, Krupa R, Wozniak K. Bisphenol A"‘glycidyl methacrylate induces a broad spectrum of DNA damage in human lymphocytes. Arch Toxicol 2011; 85:1453"‘61.

Bakopoulou A, Leyhausen G, Volk J, Koidis P, Geurtsen W. Effects of resinous monomers on the odontogenic differentiation and mineralization potential of highly proliferative and clonogenic cultured apical papilla stem cells. Dent Mater 2012; 28:327"‘39.

Reichl FX, Durner J, Kunzelmann KH, Hickel R, Spahl W, Hume WR, et al. Biological clearance of TEGDMA in guinea pigs.Arch Toxicol 2001;75:22"‘7.

Reichl FX, Durner J, Hickel R, Kunzelmann KH, Jewett A, Wang MY, et al. Distribution and excretion of TEGDMA in guinea pigs and mice. J Dent Res 2001;80:1412"‘5.

Reichl FX, Durner J, Hickel R, Spahl W, Kehe K, Walther U, et al.Uptake, clearance and metabolism of TEGDMA in guinea pigs. Dent Mater 2002;18:581"‘9.

Homa Darmani AS, Alhiyasat B. The effect of Bis"‘GMA and TEG"‘DMA on female mouse fertility. Dent Mat 2006;22:353"‘8.

Bouillaguet S, Shaw L, Gonzalez L, Kerjci I. Long term cytotoxicity of resin–based dental restorative materials. J Oral Rehab 2002;29:7"‘13.

Rathbun MA, Carig RG, Hancks CT, Filisko FE. Cytotoxicity of Bis"‘GMA dental composite before and after leaching in organic solvents. J Biomed Mater Res 1991;25:443"‘57.

Castañeda ER, Silva LA, Gaton"‘Hernández P, Consolaro A, Rodriguez EG, Silva RA, et al. Filtekâ„¢ Silorane and Filtekâ„¢ Supreme XT resins: Tissue reaction after subcutaneous implantation in isogenic mice. Braz Dent J 2011;22:105"‘10.

European Union; 2010. Available from: http: //www.bisphenol"‘a"‘europe.org. Accessed Jun 07,2012.

European Union Risk Assessment Report : 4, 4' "‘ Isopropylidenediphenol (Bisphenol"‘A); 2010. Available from http: //publications.jrc.ec.europa.eu/repository/. Accessed Jun 23, 2012.

Myers DE, Hutz RJ. Current status of potential bisphenol toxicity in dentistry. Gen Dent 2011;59:262"‘5.

Podgórski M. Structure"‘property relationship in new photo"‘cured dimethacrylate"‘based dental resins. Dent Mater 2012;28:398"‘409.

Bal BT, Sönmez N, Bavbek B, Akçaboy C. Cytotoxicity of composite resins before and after expiration date. Preliminary report. N Y State Dent J 2011;77:31"‘5.

Sigusch BW, Pflaum T, Völpel A, Gretsch K, Hoy S, Watts DC, et al.Resin"‘composite cytotoxicity varies with shade and irradiance.Dent Mater 2012;28:312"‘9.

Ergun G, Egilmez F, Cekic"‘Nagas I. The cytotoxicity of resin composites cured with three light curing units at different curing distances. Med Oral Patol Oral Cir Bucal 2011;16:e252"‘9.

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