Antioxidant and Tumor Cell Suppression Potential of Premna Serratifolia Linn Leaf
Keywords:Antioxidants, cytotoxicity, tumor, Premna serratifolia, tumor cell
AbstractHerbal and natural products have been used in folk medicine for centuries throughout the world. There has been renewed interest in screening higher plants for novel biologically active compounds, particularly those that effectively intervene in human ailments in the field of chronic diseases. The present study has been taken up to evaluate the free radical scavenging activity and tumor cell suppression potential of Premna serratifolia leaf in various in vitro model systems. The methanolic extract of P. serratifolia leaf was obtained by soxhlet extraction method. The superoxide radical scavenging activity, nitric oxide radical, hydroxyl radical, DPPH radical and ABTS radical scavenging activity and lipid peroxidation were determined. The tumor cell suppression cell potential was determined in three different cancer cell lines MCF7 (breast cancer), HepG2 (liver cancer) and A549 (lung cancer) by SRB assay. The study showed that the methanolic extract of P. serratifolia was having free radical scavenging activity against superoxide radical, nitric oxide radical, hydroxyl radical, DPPH radical, ABTS radical and inhibition of lipid peroxidation. The IC50 value showed the efficacy was dose dependent. The test extract showed cytotoxic activity against MCF7, HepG2 and A549 cells. The GI50, TGI and LC50 values were determined against each cell line and compared with standard drug Adriamycin. The present study proved the free radical scavenging activity and tumor cell suppression potential of P. serratifolia leaf in the selective in vitro model systems. The further study has to be carried out in the aspects of isolation of functional molecules of the extract.
Suffiness M, Pezzuto JM. Methods in Plant Biochemistry. Vol. 6.New York: Academic Press; 1991. p. 71.
Gulcin I, Buyukokuroglu ME, Oktay M, Kufrevioglu OI. On the in vitro antioxidant properties of melatonin. J Pineal Res 2002;33:167-71.
Halliwell B, Gutteridge JM. Free radicals in biology and medicine.Oxford: Oxford University Press; 1999.
Yildirim A, Mavi A, Oktay M, Kara AA, Algur OF, Bilaloglu V.Comparison of antioxidant and antimicrobial activities of tilia (Tilia argenta Desf Ex DC), sage (Salvia triloba L.) and black tea (Camellia sinensis) extracts. J Agric Food Chem 2000;48:5030-4.
Lopaczyski W, Zeisel SH. Antioxidants, programmed cell death and cancer. Nutr Res 2001;21:295-307.
Sgambato A, Ardito R, Faraglia B, Boninsegna A, Wolf FI, Cittadini A. Resveratrol, a natural phenolic compound, inhibits cell proliferation and prevents oxidative DNA damage. Mutat Res 2001;496:171-80.
Warrier PK, Nambiar VP, Ramankutty C. Indian Medicinal Plants.A compendium of 500 species. Vol. 4. Andhra Pradesh, India: Orient Longman Publications; 1995. p. 348-52.
Sreejayan N, Rao MN. Free radical scavenging activity by curcuminouids. Arzneimittelforschung 1996;46:169-71.
Sreejayan M, Rao MN. Nitric oxide scavenging activity by curcuminoids. J Pharm Pharmacol 1997;47:105.
Shirwaikar A, Somashekar AP. Antiinflammatory activity and free radical scavenging studies of Aristoluchia bractoelata Lam.Indian J Pharm Sci 2003;65:68.
Green LC, Wagner DA, Glogowoski J, Skipper PL, Wishnok JS, Tannenbavm SR. Analysis of nitrate and 15N in biological fluids.Anal Biochem 1982;126:131-8.
Marcocci L, Maguire JJ, Droy-Lefaix MT, Packer L. The nitric oxide scavenging property of Ginko biloba extract EGB 761. Biochim Biophys Res Commun 1994;201:748--55.
Kunchandy E, Rado MN. Oxygen radical scavenging activity of curcuminoid. Int J Pharmacogn 1990;58:237.
Sanchez-Moreno C. Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci Tech Int 2002;8:122.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351.
Prasanth Kumar V, Shasidhara S, Kumar MM, Sridhara BY.Effect of Luffa echinata on lipid peroxidation and free radical scavenging activity. J Pharm Pharmacol 2000;52:891.
Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat Protoc 2006;1:1112-6.