Bioaccumulation of lead (Pb) and remediation by calcium chelation therapy in the gills and mantle of freshwater bivalve, Lamellidens marginalis.


  • Department of Zoology, Department of Environment Science Shivaji University, Vidyanagar, Kolhapu, Maharashtra, 416004, India


The paper aims at bioaccumulation of lead (Pb) and removal of accumulated lead by calcium chelation therapy This has been studied in the gill and mantle of the freshwater bivalve, Lamellidens marginalis. The bivalves were exposed to l/W ofLC^ dose of lead acetate, different doses i.e. as 28, 56 and 84 ppm calcium acetate of and combinations of lead acetate and calcium acetate for 8 days and 16 days period. It was observed that, there was more accumulation ofPb in gills as compared to mantle. In 8 days Pb treated group accumulation of Pb in gill was 3.300 mg/ml and after 16 days exposure it was 4.129 mg/ml. It was observed that this accumulation was decreased at 56 and 84 ppm calcium treated groups for 8 days and 16 days. Similarly, in mantle Pb accumulation was 2.381 and 2.938 mg/ml on 8 days and 16 days respectively. This accumulation was decreased at 56 and 84 ppm calcium treated groups which are 1.870 and 1.581 mg/ml for 8 days and 1.20 and 1.225 for 16 days mg/ml. The study also suggests that the accumulation ofPb was directly proportional to the exposure time. With increase in calcium concentration, the concentration of Pb was decreased


Lamellidens marginalis, Lead acetate, calcium acetate. Chelation therapy

Full Text:


APHA, (2004) Standard Method for the Examination of Water and Wastewater. Amer. Public Health Association, Washington, 17th Edition.

BineyA.T.Amuzu, CalamariD., KabaN., Mbomel. L., NaeveH. and Saad M.A.H.,(1994) Review of heavy metals in the African aquatic environment, Ecotox. Environ. Saf., 28, 67-73.

Bryan, W. and Vysal, H.(1964) Heavy metals in burrowing bivalve, Scrobicularia plana from Tamar estury in relation to environmental levels. J. Mar. Biol. Assoc, 58, 89-108.

Chaudhuri, A. and Hazra, S., (2001) Trace metal accumulation in four different species of heterodont bivalve molluscs. Indian J. Environ. Protect, 25, 241-245.

Eliott, N.G., Swain, R. and Ritz D.A., (1987) Metal interaction during accumulation by the mussel Mytilus edulis planulatus. Mar Biol., 93, 395-400.

Engel, W. and Fowler, B. A. (1983) The intracellular portioning of trace metals in metals in marine shellfish. Sci. Total Environ., 28,129-140.

Flora, S J.S. and Tondon, S. K. (1991) Effect of combined exposure to lead and ethanol on some biochemical indices in the rat. Biochem. Pharmacol., 36, 537-541.

Hamilton, S.J. and Hoffman, D.J. (2003) Trace element and nutrition interactions in fish and wildlife. In: Handbook of ecotoxicology (2"" ed), (Eds.), Lewis Publishers, Boca Raton, pp 1197-1236.

Kamble, N.A. (2008) Comparative studies on induced biotoxicity of three Heavy metal Copper, Lead and Cadmium in fresh water gastropods, Ph.D. Thesis. Shivaji University, Kolhapur.

Mahaffey, K.R., Haseman, J.D. and Goyer, R.A. (1972) Dose response to lead ingested in rats fed low dietary calcium. J, Lab. Clin. Med., 83, 92-101.

Mitra, A. and Chouthary, A. (1994) Heavy metal concentration in Oyster Curassostrea cucullata. J. Ecobiol.. 6, 157159.

Narayanan, R., and Ajmalkhan, S. (1988) Cadmium induced cancer in the gills of the crab, Scylla serrate. Curr. Sci., 59, 1334-1336.

Odzak, K.T. and Cossa, R (1994) Heavy metal concentration in bivalves and gastropods, Australia Mar Freshwater Res., 49, 533-540

Phillips, J.H. (1976) The common mussels Mytilus edulis an indication of pollution by zinc, cadmium, Irad and copper. Effect of environmental variables on uptake of metals. Mar Biol., 38, 59-69.

Ponangi, S., Shams, R.A. and Joshi, S.G., (2000) Trace pollution in drinking water. J. Indian Assoc. Env. Manage., 27, 1624.

Reddy S.R. and Kumar V., (2006) Effect of chromium on protein metabolism of freshwater mussel, Lamellidens marginalls. J. Environ. Biol., 27, 401-403.

Sharma, P.D. (2001) Lead is used in storage batteries, food, beverage, ointment and medicinal concoction for flavouring and sweeting. Eco. Environ., 56, 132-138.

Sorrell, J. F. (1977) Interaction of lead, calcium, vitamin-D and nutrition in lead-burdened children. Arch. Environ. Health., 32, 160-164.

Suzuki, T, Sunaga, H., Hatakeyama, S., Sumi, Y. and Suzuki, T. (1979) Differential binding of cadmium and copper to the same protein in a heavy metal tolerant species of mayfly (Baetis thermicus) larvae. Comp. Biochem. Physiol., 94, 99-103.

Tayor.A. C, Blighand, P. and Duggan, M. (1962) The absorption of Ca, stromiun, barium and radium from the gastrointestinal tract of rat. Biochem. J., 83, 25-29.

Viarengo, A., Mancinelli, G., Martino, G., Pertica, Canesi, L. and Mazzucotelli, G. (1988a) Integrated stress indices in heavy metal contamination: their application in a field study Mar Ecol. Prog. Ser, 4, 65-70.

Viarengo, A., Mancinelli, G., Orunesu, M. and Faranda, F (1988b) Effects of sublethal copper concentrations, temperature, salinity and oxygen levels on calcium content and on cellular distribution of copper in the gills of Myhlus galloprovinc~al~Lsa m.: a multifactorial experiment. Mar Environ. Res., 24, 227-231.


  • There are currently no refbacks.