Molecular Analysis of Streptococcus thermophilus Supporting Probiotic Action towards Drug Targets


Affiliations

  • GIS, GITAM University, Department of Bioinformatics, Visakhapatnam, 530045, India

Abstract

Streptococcus thermophilus is a Gram-positive facultative anaerobe with probiotic action. Nearly 2070 genes are predicted in complete genome out of which 566 genes are related to hypothetical and uncharacterized genes. The predicted gene percent obtained by characterization of complete genome of S.thermophilus is 72.67% with 25 functional categories. There is high number of transport, protein synthesis, binding and cell envelop proteins that don't have pathogen responses. The genes ptsK, glgP, malQ, lacZ, amyL, scrB, etc are related to energy metabolism (with biodegradation and synthesis of polysaccharides) are found in chromosome CNRZ 1066. The genes such as glgP, malQ, lacZ and amyl had shown direct interactions that has not found direct link with scrB gene. All the proteins are observed with more the 50 percent identities that are present in homology models.

Keywords

Probiotics, Streptococcus thermophilus, Molecular Analysis.

Subject Discipline

Pharmacy and Pharmacology

Full Text:

References

Eisenbranda G, Pool-Zobelb B, Bakerc V, Ballsd M, Blaauboere BJ, Boobisf A, Carereg A, Kevekordesh S, Lhuguenoti JC, Pieterse R, Kleinerj J. Methods of in vitro toxicology. Food and Chemical Toxicology 2002;40(2):193-236

Dave RI, Shah NP. Viability of yoghurt and probiotic bacteria in yoghurts made from commercial starter cultures. International Dairy Journal 1997;7(1):31-41

Kaladhar DSVGK, Devi TU, Rao PN. An in silico genome wide identification, characterization and modeling of Human Papilloma Virus strain 92. International Journal of Engineering Science and Technology 2010;2(9):4288-91

Kaladhar DSVGK, Rayavarapu KA. Basics in Bioinformatics: for Students and Researchers. LAP Lambert Publishers, Germanny 2012.

Roland JS, Frank HJE, Michiel K, Bas T. Genome data mining of lactic acid bacteria: the impact of bioinformatics. Current Opinion in Biotechnology 2004;15(2):105-15

Willem MV. Advances in genomics for microbial food fermentations and safety. Current Opinion in Biotechnology 2001;12(5):493-98

Bernhard GZ, Nohjin P, David TW. Genomic Targets in Saliva. Annals of the New York Academy of Sciences 2007;1098:184-91

Callanan M, Mining the Probiotic Genome: Advanced Strategies, Enhanced Benefits, Perceived Obstacles. Current Pharmaceutical Design 2005;11(1):25-36.

Gerald WT. Probiotics and Prebiotics: Scientific Aspects. Caister Academic Press, 2005.

Kelly SS, Lawrence BS, George CF. Nutritional Genomics: Implications for Companion Animals. The American Society for Nutritional Sciences J. Nutr. 2003;133:3033-40

Hans J, Elisabeth S, Stefan R. Addition of mucin to the growth medium triggers mucus- binding activity in different strains of Lactobacillus reuteri in vitro, FEMS Microbiology Letters 2001;204(1):19–22 12. Marvin M, Kayanush JA. Influence of “Mild†Sonication Conditions on the Characteristics of Streptococcus thermophilus ST-M5. Advances in Microbiology 2012;2:8-16

Fang Y, David BP. Probiotics: progress toward novel therapies for intestinal diseases. CurrOpinGastroenterol. 2010;26(2):95-101

Verma M, Wright GL, Hanash SM, Gopal-srivastava R, Srivastava S. Proteomic Approaches within the NCI Early Detection Research Network for the Discovery and Identification of Cancer Biomarkers. Annals of the New York, Academy of Sciences 2001; 945:103-115

Maija S, SoileT, Tiina MS, Willem MV. Probiotic and other functional microbes: from markets to mechanisms. Current openion in biotechnology 2005;16(2):204-11

Todd RK, Martin JK. Selection and design of probiotics. International Journal of Food Microbiology 1999;50(1– 2):45-57

Jos B, Roland JS, Marie-Camille Z, David V, Raymond DP, Annick M, Michiel K, Willem MV, Harald B, Frank D. The complete genomes of Lactobacillus plantarum and Lactobacillus johnsonii reveal extensive differences in chromosome organization and gene content. Microbiology 2004;150:3601-11


Refbacks

  • There are currently no refbacks.