Bio Efficacy and Cry Profile of Native Bacillus thuringiensis Isolates against Cabbage Leaf Webber, Crocidolomia binotalis and Spodoptera litura Fabricius

A. Prabhakar; P. S. Hugar; P. U. Krishnaraj; A. S. Vastrad

doi:10.18311/jbc/2014/14950

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Authors

A. Prabhakar
Department of Agricultural Entomology, College of agriculture, University of Agricultural Sciences, Dharwad – 580005, Karnataka ,IN
P. S. Hugar
Department of Agricultural Entomology, College of agriculture, University of Agricultural Sciences, Dharwad – 580005, Karnataka ,IN
P. U. Krishnaraj
Department of Agricultural Entomology, College of agriculture, University of Agricultural Sciences, Dharwad – 580005, Karnataka ,IN
A. S. Vastrad
Department of Agricultural Entomology, College of agriculture, University of Agricultural Sciences, Dharwad – 580005, Karnataka ,IN

DOI:

https://doi.org/10.18311/jbc/2014/14950

Keywords:

Bacillus thuringiensis, Crocidolomia binotalis, Spodoptera litura, Cry genes.

Abstract

An investigation was carried out to assess the bio-efficacy and cry profile of hundred native Bacillus thuringiensis isolates against Cabbage Leaf Webber viz., Crocidolomia binotalis and Spodoptera litura at the Department of Entomology, University of Agricultural Sciences, Dharwad. Among the hundred native isolates used, isolates DBT-763, DBT-787, DBT-754 and DBT-2370 reported 86.67 per cent mortality against Crocidolomia binotalis and DBT-772 showed 73.33 per cent mortality against Spodoptera litura. Lepidopteron specific cry genes viz., cry1, cry2, cry8, and cry9 genes amplified in native isolates viz., DBT-2367, DBT-2366, DBT-2299, DBT-2299, respectively and none of the studied native isolates shown amplification for the cry20 gene.