Characterization of Nuclear Polyhedrosis Virus of Mythimna (Pseudaletia) Separata
Electron microscopic study of the purified nuclear polyhedrosis virus of Mythimna (Pseudaletia) separata revealed that the polyhedral inclusion bodies (PIB) contained the multiply-embedded nucleocapsid particles (MNPV) which were found enveloped. Biochemically, the proteins and nucleic acid of the virus were analyzed, respectively, for their composition or properties. The envelope and matrix proteins were studied by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The properties of the DNA of MsMNPV and HaMNPV were studied after EcoR-I treatment by agarose slab gel electrophoresis. The electrophoretic separation profile of the DNA fragments revealed a unique array of bands with respect to the molecular size.
Mythimna separata NPV, Electron Microscopy, Biochemical Characterization.
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