Antifungal activity of Bacillus subtilis subsp. spizizenii (MM19) for the management of Alternaria leaf blight of marigold

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Authors

  • R. Priyanka
     Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu ,IN
  • S. Nakkeeran
     Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu ,IN
  • I. Arumuka Pravin
     Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu ,IN
  • A. S. Krishna Moorthy
     Department of Plant Pathology, Tamil Nadu Agricultural University, Coimbatore – 641003, Tamil Nadu ,IN
  • U. Sivakumar
     Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641003, Tamil Nadu ,IN

DOI:

https://doi.org/10.18311/jbc/2018/21134

Keywords:

Alternaria, Bacillus subtilis subsp. spizizenii, GCMS, PCR

Abstract

Biological control with bioagents is a cost effective alternate method for the management of crop diseases. The antagonistic bacterial strains were explored for the management of leaf blight of marigold which is caused by Alternaria alternata. The present study clearly proved that the mycelial growth of A. alternata was inhibited up to 83% by Bacillus subtilis subsp. spizizenii (MM19) in vitro. GC/MS analysis of partially purified extracts of B. subtilis subsp. spizizenii (MM19) revealed the presence of antifungal Phthalic acid esters which might be responsible for the inhibition of the pathogen. Foliar application of B. subtilis subsp. spizizenii (MM19) under field conditions suppressed leaf blight by 77%. This study highlighted the potential of B. subtilis subsp. spizizenii (MM19) for the management of Alternaria leaf blight.

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Published

2018-08-28

How to Cite

Priyanka, R., Nakkeeran, S., Arumuka Pravin, I., Krishna Moorthy, A. S., & Sivakumar, U. (2018). Antifungal activity of <i>Bacillus subtilis</i> subsp. <i>spizizenii</i> (MM19) for the management of <i>Alternaria</i> leaf blight of marigold. Journal of Biological Control, 32(2), 95–102. https://doi.org/10.18311/jbc/2018/21134

Issue

Volume 32, Issue 2, June 2018

Section

Research Articles
Crossref
Scopus
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Europe PMC
Received 2018-05-11
Accepted 2018-07-31
Published 2018-08-28

 

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