Isolation and <i>in vitro</i> studies on indigenous fungi against root-knot nematode, <i>Meloidogyne incognita</i>

Authors

  • Nithya Dharshini K. Department of Nematology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu
  • Jothi G. Department of Nematology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu
  • Swarnakumari N. Department of Nematology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu
  • L. Rajendran Department of Plant Pathology, AC&RI, TNAU, Coimbatore – 641 003, Tamil Nadu

DOI:

https://doi.org/10.18311/jbc/2021/28554

Keywords:

Indigenous fungi, culture filtrate, <i>in vitro</i>, <i>Meloidogyne incognita</i>, <i>Talaromyces pinophilus</i>

Abstract

The present study was carried out to evaluate the indigenous fungi from soil against the root knot nematode, Meloidogyne incognita. Eleven isolates were isolated and screened for their efficacy against nematode under in vitro condition for egg hatching and juvenile mortality. The results revealed that the isolate F6 showed the highest egg hatching inhibition (88.5%) followed by F11 and F7 compared to control after incubation for 72 h. The highest juvenile mortality of 73% was recorded in isolate F6 followed by F11 and F9 after 72 h. Isolate F6 was morphologically and molecularly identified as Talaromyces pinophilus and F9 and F11 were identified as Aspergillus corrugates and Aspergillus nidulans. Among the fungi, T. pinophilus was found to be highly effective against root knot nematode.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Abdel-Rahim IR and Abo-Elyousr KA. 2018. Talaromyces pinophilus strain AUN-1 as a novel mycoparasite of Botrytis cinerea, the pathogen of onion scape and umbel blights. Microbiol. Res. 212:1–9. https://doi.org/10.1016/j.micres.2018.04.004. PMid:29853163

Bhatti DS and Jain RK. 1977. Estimation of loss in okra, tomato and brinjal yield due to Meloidogyne incognita. Indian J. Nematol. 7(1):37–41.

Eisenback JD, Hrischmann H, Sasser JN and Triantaphyllou AC. 1981. A guide to the four most common species of root-knot nematodes (Meloidogyne spp.) with a pictorial key. J. Nematol. (12):300–313.

Galvez A, Del Amor FM, Ros C and Lopez-Marin J. 2019. New traits to identify physiological responses induced by different rootstocks after root-knot nematode inoculation (Meloidogyne incognita) in sweet pepper. Crop Prot. 119:126–133. https://doi.org/10.1016/j.cropro.2019.01.026

Hamza MA, Lakhtar H, Tazi H, Moukhli A, Fossati-Gaschignard O, Miche L, et al. 2017. Diversity of nematophagous fungi in Moroccan olive nurseries: Highlighting preypredator interactions and efficient strains against root-knot nematodes. Biol. Control. 114:14–23. https://doi.org/10.1016/j.biocontrol.2017.07.011

Hussain MA, Mukhtar T and Kayani MZ. 2011. Assessment of the damage caused by Meloidogyne incognita on okra (Abelmoschus esculentus). J Anim Plant Sci. 21(4):857– 861.

Kayani MZ, Mukhtar T, Hussain MA and Ul-Haque MI.2013. Infestation assessment of root-knot nematodes (Meloidogyne spp.) associated with cucumber in the Pothowar region of Pakistan. Crop Prot. 47:49–54. https://doi.org/10.1016/j.cropro.2013.01.005

Lucas SV, Viera AA, Stchigel AM and Royo FJS. 2009. Screening culture filtrates of fungi for activity against Tylenchulus semipenetrans. Span. J. Agric. Res. 7(4):96– 904. https://doi.org/10.5424/sjar/2009074-1103

Mahfouz MMA and Askary TH. 2018. Fungal and bacterial nematicides in integrated nematode management strategies. Egypt J Biol Pest Control. 28(1):1–24. https:// doi.org/10.1186/s41938-018-0080-x

Mukhtar T, Hussain MA, Kayani MZ and Aslam MN. 2014. Evaluation of resistance to root-knot nematode (Meloidogyne incognita) in okra cultivars. Crop Prot. 56:25–30. https://doi.org/10.1016/j.cropro.2013.10.019

Noling J and Becker J. 1994. The challenge of research and extension to define and implement alternatives to methyl bromide. J. Nematol. 26(4S):573.

Pandey RK and Nayak DK. 2018. Screening and evaluation of ridge gourd varieties/cultivars against induced population of root-knot nematode, Meloidogyne incognita. J. Entomol. Zool. Stud. 6(5): 1954–1959

Sasser JN. 1979. ‘Economic Importance of Meloidogyne spp. in Tropical Countries’, in Root-Knot Nematodes (Meloidogyne spp.), Systematics, Biology and Control. eds. F Lamberti and CE Taylor. London: Academic Press. Inc.; p. 359–374.

Sikandar A, Zhang M, Wang Y, Zhu X, Liu X, Fan H and Duan Y. 2020. In vitro evaluation of Penicillium chrysogenum Snef1216 against Meloidogyne incognita (root-knot nematode). Sci. Rep. 10(1):1–9. https://doi.org/10.1038/s41598-020-65262-z. PMid:32433576. PMCid:PMC7239893

Singh S and Mathur N. 2010. In vitro studies of antagonistic fungi against the root-knot nematode, Meloidogyne incognita. Biocontrol Sci Technol. 20(3):275–282. https://doi.org/10.1080/09583150903484318

Stirling GR. 1991. Biological control of plant-parasitic nematodes: progress, problems and prospects. CAB International, Wallingford.

Stirling GR. 2014. Biological products for nematode management. Biological control of plant-parasitic nematodes: Soil ecosystem management in sustainable agriculture. (Ed. 2):342–389. https://doi.org/10.1079/9781780644158.0342

White TJ, Bruns T, Lee SJ and Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A guide to methods and applications. 18(1):315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1

Zaki MJ and Maqbool MA. 1991. Combined efficacy of Pasteuria penetrans and other biocontrol agents on the control of root-knot nematode on okra. Pak. J. Nematol. 9(1):49–52.

Downloads

Published

2022-06-05

How to Cite

Dharshini K., N., G., J., N., S., & Rajendran, L. (2022). Isolation and <i>in vitro</i> studies on indigenous fungi against root-knot nematode, <i>Meloidogyne incognita</i>. Journal of Biological Control, 35(3), 153–160. https://doi.org/10.18311/jbc/2021/28554

Issue

Section

Research Articles