Evaluation of Biocontrol Potential of Metarhizium anisopliae Strains Against Larvae and Adults of Aedes eegypti (l.)


  • National Chemical Laboratory, Division of Biochemical Sciences, Pune, MH, 400 008, India
  • National Chemical Laboratory, Laboratory of Entomology, Pune, MH, 400 008, India


In the present study, we exposed larvae and adult females of Aedes aegypti (L.) to fungal suspensions of three virulent strains of Metarhizium anisopliae (Metsch.), viz., M34412, M34311 and M81123. The strains resulted in 7 to 37% survival as a result of fungal infection over the 8 d test period in case of adults and 2 to 90% survival for larvae. Mean survival period varied between 3 and 4 d for treated adults, whilst control survival exceeded 28 d. Values of LC50 varied from 5.92×103 conidia/ml for M34412, 3.49×104 conidia/ml for M34311 and 5.12×105 conidia/ml for M81123. Based on virulence and stability, the most promising strain, M. anisopliae M34412 was used for lethal exposure time determination. An exposure time of only 4 h was necessary to cause 50% mortality. Supplementation with M. verrucaria chitinase enzyme increased the larval mortality at lower conidial concentrations.


Entomopathogen, Metarhizium anisopliae, Aedes aegypti, Biocontrol, Diptera, Culicidae.

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