Storage Stability and Performance of Aqueous and Dry Formulations of Helicoverpa armigera Nuclear Polyhedrosis Virus
Authors
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Oilseeds Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola - 444 104, Maharashtra ,IN
P. N. Mane -
Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola - 444 104, Maharashtra ,INM. P. Moharil
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Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola - 444 104, Maharashtra ,INN. S. Satpute
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Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola - 444 104, Maharashtra ,INS. M. Thakare
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Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola - 444 104, Maharashtra ,ING. K. Giri
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Department of Biotechnology, Sant Gadgebaba University, Amravati, Maharashtra ,INSwapnil Gaikwad
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Department of Biotechnology, Sant Gadgebaba University, Amravati, Maharashtra ,INA. K. Gade
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Department of Biotechnology, Sant Gadgebaba University, Amravati, Maharashtra ,INM. K. Rai
DOI:
https://doi.org/10.18311/jbc/2016/6457Keywords:
Shelf Life, Storage Stability, HaNPV Formulation, Silver Nano Particles.Abstract
Experiment on storage stability and performance of aqueous and dry formulation of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) was conducted during 2011-2013 in Bio control laboratory, Department of Entomology and Insect Biotechnology laboratory, Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola with an objective to develop potent HaNPV formulation for improving shelf life and performance. Aqueous and dry form of HaNPV formulations with antimicrobials, UV protectant and phagostimulant were prepared and studied their shelf life and performance during storage. Synthesized and characterized the Silver nanoparticles. Prepared fresh Ha NPV required for preparing different formulation. The contaminants associated, storage stability and larvicidal activity of formulated HaNPV was studied. Data revealed that, HaNPV formulations having Silver nanoparticles @ 8 μl/ml of HaNPV and 80 μl/ml of HaNPV checked the bacterial contamination up to 13 months of the storage period, did not affect the viability of POBs and insecticidal properties of HaNPV formulation. Aqueous form of HaNPV + Streptomycin @ 0.18 g/lit of HaNPV + Tinopal 1% + Sucrose 1% , dry form of HaNPV + Streptomycin @ 0.18 g/lit of HaNPV + Tinopal 1% + Sucrose 1% and HaNPV + Streptomycin @ 0.18 g/lit of HaNPV + sucrose remain stabled up to 12 months. However, aqueous form of HaNPV alone reduces the insecticidal properties from the 9th month of storage period. While, dry form of HaNPV alone reduces the insecticidal properties from the 12th month of storage period. From the result, it was noticed that dry form of HaNPV formulation having antimicrobial found more stable than aqueous form.
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