@article{Wadyalkar_Wasule_Bhoyte_Wadaskar_2003, title={Mass Multiplication and formulation of <I>Metarhizium anisopliae</I> (Metsch.) Sorokin}, volume={17}, url={https://informaticsjournals.com/index.php/jbc/article/view/3971}, DOI={10.18311/jbc/2003/3971}, abstractNote={&lt;em&gt;Metarhizium anisopliae&lt;/em&gt; is a potent microbial insecticide against &lt;em&gt;Helicoverpa armigera&lt;/em&gt; (Hübner), a potential threat to successful cultivation of many economically important crops, Pathogenicity study conducted with 10&lt;sup&gt;8&lt;/sup&gt; spores/ml concentration of fungal suspention revealed larval mortality of 100.00,90.00,76.67, and 56.67 per cent against I&lt;sup&gt;st&lt;/sup&gt;, II&lt;sup&gt;nd&lt;/sup&gt;, III&lt;sup&gt;rd&lt;/sup&gt; and IV&lt;sup&gt;th&lt;/sup&gt; instar larvae of &lt;em&gt;H. armigera&lt;/em&gt;, respectively. Five media were compared at four temperature levels. Emersons’s Yeast Phosphate Soluble Starch (YPSS) medium recorded highest conidial count of 22.00 x 10&lt;sup&gt;7&lt;/sup&gt; spores/ml at 27° C followed. by 25&lt;sup&gt;0&lt;/sup&gt; C, Sabouraud’s Dextrose Broth + Yeast (SDB+Y) and Potato Dextrose Broth (PDB) being on par with YPSS at 27&lt;sup&gt;0&lt;/sup&gt; C. Poor cultural growth was recorded in Barners medium at all temperature levels. Amongst the five media used for the mass multiplication studies, Potato Dextrose Broth was found to be hetter in terms of spore production, viability of conidia and its production cost. Bioassay data analyzed by log dose Probit method revealed LC&lt;sub&gt;50&lt;/sub&gt; values of 6.0x10&lt;sup&gt;4&lt;/sup&gt;, 1.2xlO&lt;sup&gt;6&lt;/sup&gt;, 4.8x10&lt;sup&gt;6&lt;/sup&gt; and 5.0x10&lt;sup&gt;7&lt;/sup&gt; spores/ml for I&lt;sup&gt;st&lt;/sup&gt;, II&lt;sup&gt;nd&lt;/sup&gt;, III&lt;sup&gt;rd&lt;/sup&gt; and IV&lt;sup&gt;th&lt;/sup&gt; instar &lt;em&gt;H. armigera&lt;/em&gt; larvae, respectively, with very shallow slopes.}, number={2}, journal={Journal of Biological Control}, author={Wadyalkar, S. R. and Wasule, D. L. and Bhoyte, S. J. and Wadaskar, R. M.}, year={2003}, month={Dec.}, pages={141–146} }