TY - JOUR AU - Kaur, M. AU - Joshi, Neelam AU - Sharma, S. AU - Kalia, A. PY - 2021/12/01 Y2 - 2024/03/29 TI - Pathogenicity of Nucleopolyhedrovirus (NPV) against Spodoptera litura (Fabricius) JF - Journal of Biological Control JA - JBC VL - 35 IS - 4 SE - Research Articles DO - 10.18311/jbc/2021/29342 UR - https://informaticsjournals.com/index.php/jbc/article/view/29342 SP - 218-226 AB - <p>The management of <em>Spodoptera litura</em> by entomopathogenic Nucleopolyhedrosis Viruses (NPVs) is one of the eco-friendly methods. The present study was aimed to evaluate Nucleopolyhedrosis Virus (NPV) against <em>S. litura</em> and its potential as a bio-pesticide. The Occlusion Bodies (OBs) of <em>S. litura</em> nucleopolyhedrosis virus was isolated from infected larvae collected from cabbage field. Pathogenicity studies evaluated three NPV suspensions <em>viz.</em> SpltNPV-native, SpltNPV-commercial and SpltNPV-NIPHM against second and fourth instar larvae of <em>S. litura</em> recorded maximum mortality at 1×10<sup>9</sup> OBs/ml. The LC<sub>50</sub> values of the SpltNPV-native, SpltNPV-commercial and SpltNPV-NIPHM suspensions against second instar larvae were 0.584, 0.540, 0.625 OBs/mm<sup>2</sup>, respectively, which increased to 0.696, 0.620, 0.756 OBs/mm<sup>2</sup> against the fourth instar larvae. The LT<sub>50</sub> at 1×10<sup>9</sup> OBs/ml was found to increase from 146.33, 137.51 and 155.88 h for SpltNPV-native, SpltNPV-commercial and SpltNPV-NIPHM suspension, respectively, against the second instar larvae to 178.51, 162.07 and 187.67 h, respectively, against the fourth instar larvae. The cumulative per cent mortality, LC<sub>50</sub> and LT<sub>50</sub> suggested that the second instar larvae were more susceptible and easier to kill than the fourth instar larvae.</p> ER -